LOCUS V026968 9199 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026968 VERSION V026968 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..3895 /label="PTGR2(NM_152444)" /note="PTGR2(NM_152444)" /gene="PTGR2" CDS 3902..3967 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 3987..4486 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 4507..5106 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5120..5708 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5915..6548 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6677..6693) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6701..6717 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6725..6755) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6770..6791 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7079..7667) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7838..8698) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8699..8803) /gene="bla" /label="AmpR promoter" polyA_signal 8851..8985 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgattgt tcaaagagtg gtattgaatt ctcgacctgg 2881 aaaaaatggt aatccagtgg cagagaattt ccgaatggaa gaagtctatt taccagataa 2941 tattaatgaa ggacaagtac aagttagaac tctttatctt tctgtggatc cttacatgcg 3001 ttgtagaatg aatgaagaca ctggcactga ttatataaca ccttggcagc tatctcaagt 3061 cgttgatgga ggaggtattg gaattataga agaaagcaaa cacacaaatt tgactaaagg 3121 cgattttgtg acttctttct attggccctg gcaaaccaag gttattctgg atggaaatag 3181 ccttgaaaag gtagacccac aacttgtgga tggacacctt tcatattttc ttggagctat 3241 aggtatgcct ggtttgactt ccttgattgg gatacaggaa aaaggtcata taactgctgg 3301 atctaataag acaatggttg tcagtggggc cgcaggtgcc tgtggatctg tggctgggca 3361 gattggccat ttcttaggtt gttccagagt ggtgggaatt tgtggaacac atgagaaatg 3421 catcctcttg acctcagaac tgggctttga tgctgcaatt aattataaaa aagacaatgt 3481 ggcagaacag ctccgtgaat catgcccagc tggagtggat gtttattttg acaatgttgg 3541 tggtaacatc agtgatacag tgataagtca gatgaatgag aacagccaca tcatcctgtg 3601 tggtcaaatt tctcagtaca acaaagatgt gccttatcct cccccgctat cccctgctat 3661 agaggcaatc cagaaagaaa gaaacatcac aagggaaaga tttctggtat taaattataa 3721 agacaaattt gagcctggca ttctacagct gagtcagtgg tttaaagaag gaaagctaaa 3781 gattaaagag acggtaataa atgggttgga aaacatggga gctgcattcc agtccatgat 3841 gacaggaggt aacattggaa agcagatagt ttgcatttca gaagaaatct ctttgggatc 3901 cgactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga 3961 tgacaagtag tctagataat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg 4021 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg 4081 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc 4141 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg 4201 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc 4261 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc 4321 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag 4381 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg 4441 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag 4501 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 4561 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 4621 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 4681 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 4741 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 4801 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 4861 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 4921 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 4981 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 5041 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 5101 gcctgaccgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg 5161 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 5221 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 5281 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 5341 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 5401 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 5461 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 5521 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 5581 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 5641 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 5701 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt 5761 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag 5821 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta 5881 gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga 5941 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag 6001 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta 6061 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta 6121 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt 6181 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac 6241 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc 6301 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc 6361 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 6421 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 6481 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 6541 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 6601 aatatcagag agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga 6661 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 6721 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 6781 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 6841 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 6901 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 6961 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 7021 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 7081 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 7141 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 7201 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 7261 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 7321 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 7381 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 7441 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 7501 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 7561 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 7621 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 7681 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 7741 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 7801 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 7861 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 7921 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 7981 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 8041 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 8101 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 8161 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 8221 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 8281 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 8341 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 8401 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 8461 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 8521 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 8581 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 8641 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 8701 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 8761 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 8821 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac 8881 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 8941 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa 9001 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg 9061 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt 9121 atttgttaaa tatgtactac aaacttagta gtttttaaag aaattgtatt tgttaaatat 9181 gtactacaaa cttagtagt //