LOCUS V023371 10801 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V023371 VERSION V023371 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..5476 /label="PRKD2(NM_016457)" /note="PRKD2(NM_016457)" /gene="PRKD2" CDS 5483..5509 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 5513..5539 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 5543..5569 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 5589..6088 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 6109..6708 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6722..7310 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7517..8150 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(8279..8295) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8303..8319 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8327..8357) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8372..8393 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8681..9269) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9440..10300) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10301..10405) /gene="bla" /label="AmpR promoter" polyA_signal 10453..10587 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggccac cgccccctct tatcccgccg ggctccctgg 2881 ctctcccggg ccggggtctc ctccgccccc cggcggccta gagctgcagt cgccgccacc 2941 gctactgccc cagatcccgg ccccgggttc cggggtctcc tttcacatcc agatcgggct 3001 gacccgcgag ttcgtgctgt tgcccgccgc ctccgagctg gctcatgtga agcagctggc 3061 ctgttccatc gtggaccaga agttccctga gtgtggcttc tacggccttt acgacaagat 3121 cctgcttttc aaacatgacc ccacgtcggc caacctcctg cagctggtgc gctcgtccgg 3181 agacatccag gagggcgacc tggtggaggt ggtgctgtcg gcctcggcca ccttcgagga 3241 cttccagatc cgcccgcacg ccctcacggt gcactcctat cgggcgcctg ccttctgtga 3301 tcactgcggg gagatgctct tcggcctagt gcgccagggc ctcaagtgcg atggctgcgg 3361 gctgaactac cacaagcgct gtgccttcag catccccaac aactgtagtg gggcccgcaa 3421 acggcgcctg tcatccacgt ctctggccag tggccactcg gtgcgcctcg gcacctccga 3481 gtccctgccc tgcacggctg aagagctgag ccgtagcacc accgaactcc tgcctcgccg 3541 tcccccgtca tcctcttcct cctcttctgc ctcatcgtat acgggccgcc ccattgagct 3601 ggacaagatg ctgctctcca aggtcaaggt gccgcacacc ttcctcatcc acagctatac 3661 acggcccacc gtttgccagg cttgcaagaa actcctcaag ggcctcttcc ggcagggcct 3721 gcaatgcaaa gactgcaagt ttaactgtca caaacgctgc gccacccgcg tccctaatga 3781 ctgcctgggg gaggccctta tcaatggaga tgtgccgatg gaggaggcca ccgatttcag 3841 cgaggctgac aagagcgccc tcatggatga gtcagaggac tccggtgtca tccctggctc 3901 ccactcagag aatgcgctcc acgccagtga ggaggaggaa ggcgagggag gcaaggccca 3961 gagctccctg gggtacatcc ccctaatgag ggtggtgcaa tcggtgcgac acacgacgcg 4021 gaaatccagc accacgctgc gggagggttg ggtggttcat tacagcaaca aggacacgct 4081 gagaaagcgg cactattggc gcctggactg caagtgtatc acgctcttcc agaacaacac 4141 gaccaacaga tactataagg aaattccgct gtcagaaatc ctcacggtgg agtccgccca 4201 gaacttcagc cttgtgccgc cgggcaccaa cccacactgc tttgagatcg tcactgccaa 4261 tgccacctac ttcgtgggcg agatgcctgg cgggactccg ggtgggccaa gtgggcaggg 4321 ggctgaggcc gcccggggct gggagacagc catccgccag gccctgatgc ccgtcatcct 4381 tcaggacgca cccagcgccc caggccacgc gccccacaga caagcttctc tgagcatctc 4441 tgtgtccaac agtcagatcc aagagaatgt ggacattgcc actgtctacc agatcttccc 4501 tgacgaagtg ctgggctcag ggcagtttgg agtggtctat ggaggaaaac accggaagac 4561 aggccgggac gtggcagtta aggtcattga caaactgcgc ttccctacca agcaggagag 4621 ccagctccgg aatgaagtgg ccattctgca gagcctgcgg catcccggga tcgtgaacct 4681 ggagtgcatg ttcgagacgc ctgagaaagt gtttgtggtg atggagaagc tgcatgggga 4741 catgttggag atgatcctgt ccagtgagaa gggccggctg cctgagcgcc tcaccaagtt 4801 cctcatcacc cagatcctgg tggctttgag acaccttcac ttcaagaaca ttgtccactg 4861 tgacttgaaa ccagaaaacg tgttgctggc atcagcagac ccatttcctc aggtgaagct 4921 gtgtgacttt ggctttgctc gcatcatcgg cgagaagtcg ttccgccgct cagtggtggg 4981 cacgccggcc tacctggcac ccgaggtgct gctcaaccag ggctacaacc gctcgctgga 5041 catgtggtca gtgggcgtga tcatgtacgt cagcctcagc ggcaccttcc ctttcaacga 5101 ggatgaggac atcaatgacc agatccagaa cgccgccttc atgtacccgg ccagcccctg 5161 gagccacatc tcagctggag ccattgacct catcaacaac ctgctgcagg tgaagatgcg 5221 caaacgctac agcgtggaca aatctctcag ccacccctgg ttacaggagt accagacgtg 5281 gctggacctc cgagagctgg aggggaagat gggagagcga tacatcacgc atgagagtga 5341 cgacgcgcgc tgggagcagt ttgcagcaga gcatccgctg cctgggtctg ggctgcccac 5401 ggacagggat ctcggtgggg cctgtccacc acaggaccac gacatgcagg ggctggcgga 5461 gcgcatcagt gttctcggat cctacccata cgacgtacct gattatgcaa cttatcctta 5521 tgatgtacct gattatgcta gctatcctta tgatgtccca gactacgcat agtctagata 5581 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 5641 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 5701 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 5761 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 5821 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 5881 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 5941 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 6001 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 6061 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac 6121 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 6181 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 6241 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 6301 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 6361 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 6421 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 6481 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 6541 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 6601 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 6661 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa 6721 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 6781 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 6841 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 6901 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 6961 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 7021 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 7081 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 7141 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 7201 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 7261 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa 7321 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc 7381 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca 7441 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 7501 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat 7561 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca 7621 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat 7681 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat 7741 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt 7801 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc 7861 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag 7921 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg 7981 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 8041 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 8101 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 8161 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 8221 gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg taatcatggt 8281 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 8341 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 8401 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 8461 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 8521 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 8581 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 8641 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 8701 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 8761 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 8821 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 8881 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 8941 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 9001 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 9061 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 9121 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 9181 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 9241 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 9301 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 9361 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 9421 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 9481 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 9541 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 9601 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 9661 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 9721 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 9781 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 9841 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 9901 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 9961 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 10021 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 10081 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 10141 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 10201 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 10261 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 10321 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 10381 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg 10441 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 10501 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 10561 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc 10621 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa 10681 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact 10741 acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag 10801 t //