LOCUS       V027762                10156 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027762
VERSION     V027762
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..4831
                     /label="PLEKHN1(NM_001410697)"
                     /note="PLEKHN1(NM_001410697)"
                     /gene="PLEKHN1"
     CDS             4838..4864
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4868..4894
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4898..4924
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4944..5443
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5464..6063
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6077..6665
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6872..7505
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7634..7650)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7658..7674
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7682..7712)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7727..7748
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8036..8624)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8795..9655)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9656..9760)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9808..9942
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatggggaa cagccactgt gtccctcagg cccccaggag
     2881 gctccgggcc tccttctcca gaaagccctc gctgaaggga aacagagagg acagcgcgcg
     2941 gatgtcggcc ggcctgccgg gccccgaggc tgctcgaagc ggggacgccg ccgccaacaa
     3001 gctcttccac tacatcccgg gcacggacat cctggacctg gagaaccagc gagaaaacct
     3061 ggagcagcca ttcctgagtg tgttcaagaa ggggcggcgg agggtgcctg tgaggaacct
     3121 gggaaaagtt gtgcattacg ccaaggtcca gctgcggttc cagcacagcc aggatgtcag
     3181 cgactgctac ctggagctat tccccgccca cctgtacttc caggcccacg gctcggaagg
     3241 actcacattt caggggctgt taccgctgac ggagctgagt gtctgcccgc tcgaggggtc
     3301 ccgagagcac gccttccaga tcacaggtgt ttgggatgct tcccgggccc ccagaggcac
     3361 tcctgaccca ggacttggag aggggcctgc cctgtggctg cggagcacgt gtgtgtatgt
     3421 gtgtgccctc tctgccctgc ccgcaggccc actgcccgca cccctcctgg tgctctgccc
     3481 cagccgggcc gagctggacc gctggcttta ccacctggag aagcagacgg ccctcctcgg
     3541 ggggccgcgg cgctgccact cggcaccccc acaggggtcc tgcggagacg aactcccctg
     3601 gactttgcag cgccgtctaa cccggctgcg gacggcgtca gggcacgaac ccggcggcag
     3661 tgctgtctgt gcctcgaggg tcaagctgca gcacctgccc gcacaggagc agtgggaccg
     3721 gctcttggtc ctgtacccaa cgtccttggc cattttctcc gaggagctgg acgggctttg
     3781 cttcaagggg gagctcccac tccgtgccgt ccacatcaac ctggaggaga aggagaagca
     3841 gatccgctcc ttcctgattg aaggccccct catcaacacc atccgcgtgg tgtgcgccag
     3901 ctacgaggac tacggtcact ggctgctgtg ccttcgcgct gtcacccaca gggagggggc
     3961 cccgccgctg cctggtgccg agagcttccc agggtcgcag gttatgggca gtggccgagg
     4021 ctcactctcc tcaggcggac agaccagctg ggactcgggg tgcttggcgc ccccctccac
     4081 ccgcaccagc cactccctgc ctgagtcctc agtgccatcc accgtgggct gctcctccca
     4141 gcacacaccg gaccaggcca actctgaccg tgccagcatt ggccgacgga ggaccgagct
     4201 gagacgcagt ggcagcagcc ggtcacccgg gagcaaggcc cgggcagagg gccgcggccc
     4261 tgtcacccca ctgcacctgg acctgaccca gctgcacagg ctgagcctgg agagcagccc
     4321 agatgcccct gaccacactt cggaaacatc acactcgccc ctctatgccg acccctacac
     4381 accacccgcc acctcccacc gcagggtcac agatgtccgg ggcctggagg agttcctcag
     4441 tgccatgcag agtgcacgtg gacccacgcc ctcgagccca ctcccctcgg tgcctgtgtc
     4501 tgtgcctgcc tctgaccctc gctcctgctc ctccggcccc gctggcccct acttgctctc
     4561 caagaaggga gccctgcagt ccagagccgc tcagagacac cggggctcag ccaaggatgg
     4621 ggggccgcag cccccagacg cccctcagct tgtctcctct gccagggaag gttcgcccga
     4681 accctggctg cctctgacag atggtcggtc ccccaggagg agccgggacc ccggctacga
     4741 ccacctctgg gacgagactt tgtcttcctc ccaccagaag tgcccccagc ttggagggcc
     4801 tgaggccagt ggggggcttg tgcagtggat cggatcctac ccatacgacg tacctgatta
     4861 tgcaacttat ccttatgatg tacctgatta tgctagctat ccttatgatg tcccagacta
     4921 cgcatagtct agataattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     4981 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     5041 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     5101 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     5161 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     5221 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     5281 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     5341 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     5401 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
     5461 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     5521 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     5581 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     5641 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     5701 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     5761 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     5821 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     5881 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     5941 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     6001 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     6061 tgaccgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6121 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6181 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6241 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6301 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6361 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     6421 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     6481 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     6541 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     6601 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     6661 cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc
     6721 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     6781 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     6841 cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga
     6901 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
     6961 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
     7021 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
     7081 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
     7141 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
     7201 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
     7261 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
     7321 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7381 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7441 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7501 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     7561 atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct agctagagct
     7621 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7681 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7741 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7801 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7861 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7921 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7981 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8041 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8101 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8161 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8221 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8281 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8341 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8401 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8461 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8521 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8581 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8641 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8701 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8761 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8821 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8881 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8941 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9001 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9061 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9121 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9181 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9241 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9301 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9361 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9421 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9481 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9541 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9601 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9661 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9721 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9781 cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa
     9841 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9901 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
     9961 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
    10021 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
    10081 tgttaaatat gtactacaaa cttagtagtt tttaaagaaa ttgtatttgt taaatatgta
    10141 ctacaaactt agtagt
//
