LOCUS       V028137                11188 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028137
VERSION     V028137
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..5884
                     /label="PARP1(NM_001618)"
                     /note="PARP1(NM_001618)"
                     /gene="PARP1"
     CDS             5891..5956
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5976..6475
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6496..7095
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7109..7697
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7904..8537
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8666..8682)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8690..8706
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8714..8744)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8759..8780
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9068..9656)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9827..10687)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10688..10792)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10840..10974
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatggcgga gtcttcggat aagctctatc gagtcgagta
     2881 cgccaagagc gggcgcgcct cttgcaagaa atgcagcgag agcatcccca aggactcgct
     2941 ccggatggcc atcatggtgc agtcgcccat gtttgatgga aaagtcccac actggtacca
     3001 cttctcctgc ttctggaagg tgggccactc catccggcac cctgacgttg aggtggatgg
     3061 gttctctgag cttcggtggg atgaccagca gaaagtcaag aagacagcgg aagctggagg
     3121 agtgacaggc aaaggccagg atggaattgg tagcaaggca gagaagactc tgggtgactt
     3181 tgcagcagag tatgccaagt ccaacagaag tacgtgcaag gggtgtatgg agaagataga
     3241 aaagggccag gtgcgcctgt ccaagaagat ggtggacccg gagaagccac agctaggcat
     3301 gattgaccgc tggtaccatc caggctgctt tgtcaagaac agggaggagc tgggtttccg
     3361 gcccgagtac agtgcgagtc agctcaaggg cttcagcctc cttgctacag aggataaaga
     3421 agccctgaag aagcagctcc caggagtcaa gagtgaagga aagagaaaag gcgatgaggt
     3481 ggatggagtg gatgaagtgg cgaagaagaa atctaaaaaa gaaaaagaca aggatagtaa
     3541 gcttgaaaaa gccctaaagg ctcagaacga cctgatctgg aacatcaagg acgagctaaa
     3601 gaaagtgtgt tcaactaatg acctgaagga gctactcatc ttcaacaagc agcaagtgcc
     3661 ttctggggag tcggcgatct tggaccgagt agctgatggc atggtgttcg gtgccctcct
     3721 tccctgcgag gaatgctcgg gtcagctggt cttcaagagc gatgcctatt actgcactgg
     3781 ggacgtcact gcctggacca agtgtatggt caagacacag acacccaacc ggaaggagtg
     3841 ggtaacccca aaggaattcc gagaaatctc ttacctcaag aaattgaagg ttaaaaaaca
     3901 ggaccgtata ttccccccag aaaccagcgc ctccgtggcg gccacgcctc cgccctccac
     3961 agcctcggct cctgctgctg tgaactcctc tgcttcagca gataagccat tatccaacat
     4021 gaagatcctg actctcggga agctgtcccg gaacaaggat gaagtgaagg ccatgattga
     4081 gaaactcggg gggaagttga cggggacggc caacaaggct tccctgtgca tcagcaccaa
     4141 aaaggaggtg gaaaagatga ataagaagat ggaggaagta aaggaagcca acatccgagt
     4201 tgtgtctgag gacttcctcc aggacgtctc cgcctccacc aagagccttc aggagttgtt
     4261 cttagcgcac atcttgtccc cttggggggc agaggtgaag gcagagcctg ttgaagttgt
     4321 ggccccaaga gggaagtcag gggctgcgct ctccaaaaaa agcaagggcc aggtcaagga
     4381 ggaaggtatc aacaaatctg aaaagagaat gaaattaact cttaaaggag gagcagctgt
     4441 ggatcctgat tctggactgg aacactctgc gcatgtcctg gagaaaggtg ggaaggtctt
     4501 cagtgccacc cttggcctgg tggacatcgt taaaggaacc aactcctact acaagctgca
     4561 gcttctggag gacgacaagg aaaacaggta ttggatattc aggtcctggg gccgtgtggg
     4621 tacggtgatc ggtagcaaca aactggaaca gatgccgtcc aaggaggatg ccattgagca
     4681 cttcatgaaa ttatatgaag aaaaaaccgg gaacgcttgg cactccaaaa atttcacgaa
     4741 gtatcccaaa aagttctacc ccctggagat tgactatggc caggatgaag aggcagtgaa
     4801 gaagctgaca gtaaatcctg gcaccaagtc caagctcccc aagccagttc aggacctcat
     4861 caagatgatc tttgatgtgg aaagtatgaa gaaagccatg gtggagtatg agatcgacct
     4921 tcagaagatg cccttgggga agctgagcaa aaggcagatc caggccgcat actccatcct
     4981 cagtgaggtc cagcaggcgg tgtctcaggg cagcagcgac tctcagatcc tggatctctc
     5041 aaatcgcttt tacaccctga tcccccacga ctttgggatg aagaagcctc cgctcctgaa
     5101 caatgcagac agtgtgcagg ccaaggtgga aatgcttgac aacctgctgg acatcgaggt
     5161 ggcctacagt ctgctcaggg gagggtctga tgatagcagc aaggatccca tcgatgtcaa
     5221 ctatgagaag ctcaaaactg acattaaggt ggttgacaga gattctgaag aagccgagat
     5281 catcaggaag tatgttaaga acactcatgc aaccacacac aatgcgtatg acttggaagt
     5341 catcgatatc tttaagatag agcgtgaagg cgaatgccag cgttacaagc cctttaagca
     5401 gcttcataac cgaagattgc tgtggcacgg gtccaggacc accaactttg ctgggatcct
     5461 gtcccagggt cttcggatag ccccgcctga agcgcccgtg acaggctaca tgtttggtaa
     5521 agggatctat ttcgctgaca tggtctccaa gagtgccaac tactgccata cgtctcaggg
     5581 agacccaata ggcttaatcc tgttgggaga agttgccctt ggaaacatgt atgaactgaa
     5641 gcacgcttca catatcagca agttacccaa gggcaagcac agtgtcaaag gtttgggcaa
     5701 aactacccct gatccttcag ctaacattag tctggatggt gtagacgttc ctcttgggac
     5761 cgggatttca tctggtgtga atgacacctc tctactatat aacgagtaca ttgtctatga
     5821 tattgctcag gtaaatctga agtatctgct gaaactgaaa ttcaatttta agacctccct
     5881 gtggggatcc gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     5941 ggatgacgat gacaagtagt ctagataatt ctaccgggta ggggaggcgc ttttcccaag
     6001 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     6061 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     6121 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     6181 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     6241 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     6301 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     6361 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     6421 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     6481 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6541 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6601 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6661 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6721 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6781 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6841 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6901 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6961 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     7021 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     7081 aagcccggtg cctgaccgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag
     7141 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7201 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7261 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7321 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     7381 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     7441 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7501 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7561 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     7621 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7681 ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca
     7741 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     7801 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     7861 gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac
     7921 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     7981 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
     8041 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
     8101 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
     8161 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
     8221 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
     8281 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
     8341 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8401 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8461 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8521 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     8581 aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttta ccgtcgacct
     8641 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8701 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8761 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8821 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8881 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8941 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9001 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9061 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9121 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9181 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9241 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9301 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9361 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9421 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9481 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     9541 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9601 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9661 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9721 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9781 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9841 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9901 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9961 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10021 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10081 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10141 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10201 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10261 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10321 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10381 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10441 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10501 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10561 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10621 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10681 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10741 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10801 cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat
    10861 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    10921 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    10981 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    11041 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    11101 agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt
    11161 gttaaatatg tactacaaac ttagtagt
//
