LOCUS       V030336                11026 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030336
VERSION     V030336
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..5722
                     /label="OPA1(NM_015560)"
                     /note="OPA1(NM_015560)"
                     /gene="OPA1"
     CDS             5729..5794
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5814..6313
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6334..6933
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6947..7535
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7742..8375
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8504..8520)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8528..8544
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8552..8582)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8597..8618
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8906..9494)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9665..10525)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10526..10630)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10678..10812
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgtggcg actacgtcgg gccgctgtgg cctgtgaggt
     2881 ctgccagtct ttagtgaaac acagctctgg aataaaagga agtttaccac tacaaaaact
     2941 acatctggtt tcacgaagca tttatcattc acatcatcct accttaaagc ttcaacgacc
     3001 ccaattaagg acatcctttc agcagttctc ttctctgaca aaccttcctt tacgtaaact
     3061 gaaattctct ccaattaaat atggctacca gcctcgcagg aatttttggc cagcaagatt
     3121 agctacgaga ctcttaaaac ttcgctatct catactagga tcggctgttg ggggtggcta
     3181 cacagccaaa aagacttttg atcagtggaa agatatgata ccggacctta gtgaatataa
     3241 atggattgtg cctgacattg tgtgggaaat tgatgagtat atcgattttg agaaaattag
     3301 aaaagccctt cctagttcag aagaccttgt aaagttagca ccagactttg acaagattgt
     3361 tgaaagcctt agcttattga aggacttttt tacctcaggt tctccggaag aaacggcgtt
     3421 tagagcaaca gatcgtggat ctgaaagtga caagcatttt agaaaggtgt cagacaaaga
     3481 gaaaattgac caacttcagg aagaacttct gcacactcag ttgaagtatc agagaatctt
     3541 ggaacgatta gaaaaggaga acaaagaatt gagaaaatta gtattgcaga aagatgacaa
     3601 aggcattcat catagaaagc ttaagaaatc tttgattgac atgtattctg aagttcttga
     3661 tgttctctct gattatgatg ccagttataa tacgcaagat catctgccac gggttgttgt
     3721 ggttggagat cagagtgctg gaaagactag tgtgttggaa atgattgccc aagctcgaat
     3781 attcccaaga ggatctgggg agatgatgac acgttctcca gttaaggtga ctctgagtga
     3841 aggtcctcac catgtggccc tatttaaaga tagttctcgg gagtttgatc ttaccaaaga
     3901 agaagatctt gcagcattaa gacatgaaat agaacttcga atgaggaaaa atgtgaaaga
     3961 aggctgtacc gttagccctg agaccatatc cttaaatgta aaaggccctg gactacagag
     4021 gatggtgctt gttgacttac caggtgtgat taatactgtg acatcaggca tggctcctga
     4081 cacaaaggaa actattttca gtatcagcaa agcttacatg cagaatccta atgccatcat
     4141 actgtgtatt caagatggat ctgtggatgc tgaacgcagt attgttacag acttggtcag
     4201 tcaaatggac cctcatggaa ggagaaccat attcgttttg accaaagtag acctggcaga
     4261 gaaaaatgta gccagtccaa gcaggattca gcagataatt gaaggaaagc tcttcccaat
     4321 gaaagcttta ggttattttg ctgttgtaac aggaaaaggg aacagctctg aaagcattga
     4381 agctataaga gaatatgaag aagagttttt tcagaattca aagctcctaa agacaagcat
     4441 gctaaaggca caccaagtga ctacaagaaa tttaagcctt gcagtatcag actgcttttg
     4501 gaaaatggta cgagagtctg ttgaacaaca ggctgatagt ttcaaagcaa cacgttttaa
     4561 ccttgaaact gaatggaaga ataactatcc tcgcctgcgg gaacttgacc ggaatgaact
     4621 atttgaaaaa gctaaaaatg aaatccttga tgaagttatc agtctgagcc aggttacacc
     4681 aaaacattgg gaggaaatcc ttcaacaatc tttgtgggaa agagtatcaa ctcatgtgat
     4741 tgaaaacatc taccttccag ctgcgcagac catgaattca ggaactttta acaccacagt
     4801 ggatatcaag cttaaacagt ggactgataa acaacttcct aataaagcag tagaggttgc
     4861 ttgggagacc ctacaagaag aattttcccg ctttatgaca gaaccgaaag ggaaagagca
     4921 tgatgacata tttgataaac ttaaagaggc tgttaaggaa gaaagtatta aacgacacaa
     4981 gtggaatgac tttgcggagg acagcttgag ggttattcaa cacaatgctt tggaagaccg
     5041 atccatatct gataaacagc aatgggatgc agctatttat tttatggaag aggctctgca
     5101 ggctcgtctc aaggatactg aaaatgcaat tgaaaacatg gtgggtccag actggaaaaa
     5161 gaggtggtta tactggaaga atcggaccca agaacagtgt gttcacaatg aaaccaagaa
     5221 tgaattggag aagatgttga aatgtaatga ggagcaccca gcttatcttg caagtgatga
     5281 aataaccaca gtccggaaga accttgaatc ccgaggagta gaagtagatc caagcttgat
     5341 taaggatact tggcatcaag tttatagaag acatttttta aaaacagctc taaaccattg
     5401 taacctttgt cgaagaggtt tttattacta ccaaaggcat tttgtagatt ctgagttgga
     5461 atgcaatgat gtggtcttgt tttggcgtat acagcgcatg cttgctatca ccgcaaatac
     5521 tttaaggcaa caacttacaa atactgaagt taggcgatta gagaaaaatg ttaaagaggt
     5581 attggaagat tttgctgaag atggtgagaa gaagattaaa ttgcttactg gtaaacgcgt
     5641 tcaactggcg gaagacctca agaaagttag agaaattcaa gaaaaacttg atgctttcat
     5701 tgaagctctt catcaggaga aaggatccga ctacaaagac catgacggtg attataaaga
     5761 tcatgacatc gactacaagg atgacgatga caagtagtct agataattct accgggtagg
     5821 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
     5881 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     5941 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     6001 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     6061 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     6121 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     6181 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     6241 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     6301 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc
     6361 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6421 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6481 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     6541 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     6601 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6661 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6721 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6781 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     6841 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     6901 acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga
     6961 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7021 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7081 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7141 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7201 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7261 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7321 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     7381 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7441 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7501 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac
     7561 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     7621 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     7681 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga
     7741 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     7801 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     7861 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     7921 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     7981 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     8041 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
     8101 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
     8161 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
     8221 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8281 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8341 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     8401 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta
     8461 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     8521 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8581 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8641 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8701 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8761 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8821 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8881 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8941 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9001 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9061 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9121 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9181 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9241 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9301 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9361 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9421 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9481 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9541 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9601 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9661 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9721 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9781 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9841 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9901 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9961 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10021 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10081 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10141 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10201 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10261 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10321 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10381 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10441 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10501 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10561 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10621 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac
    10681 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    10741 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    10801 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    10861 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    10921 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
    10981 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
