LOCUS       V018335                11206 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018335
VERSION     V018335
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2827..5925
                     /label="Nlrp3(NM_145827)"
                     /note="Nlrp3(NM_145827)"
                     /gene="Nlrp3"
     CDS             5926..5955
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     promoter        5994..6493
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6514..7113
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7127..7715
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7922..8555
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8684..8700)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8708..8724
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8732..8762)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8777..8798
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9086..9674)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9845..10705)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10706..10810)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10858..10992
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatga cgagtgtccg ttgcaagctg gctcagtatc tagaggacct tgaagatgtg
     2881 gacctcaaga aattcaaaat gcatttggaa gattacccgc ccgagaaagg ctgtatccca
     2941 gtccccaggg gccagatgga gaaggcagat cacttggatc tagccacact catgattgac
     3001 ttcaatggcg aggagaaggc ctgggccatg gctgtgtgga tctttgctgc gatcaacagg
     3061 cgagacctct gggaaaaagc taagaaggac cagccagagt ggaatgacac gtgtacatca
     3121 cattcctcta tggtatgcca ggaggacagc cttgaagaag agtggatggg tttgctggga
     3181 tatctctccc gcatctccat ttgtaaaaag aagaaagatt actgtaagat gtacagacga
     3241 catgtgagaa gcaggttcta ctctatcaag gacaggaacg cgcgtctagg tgagagtgtg
     3301 gacctcaaca gtcgctacac gcagctccaa ctggtcaagg agcatccaag caagcaggag
     3361 cgggagcatg aactcctgac catcggccgg actaaaatgc gggacagccc catgagttcc
     3421 cttaagctgg agctgctgtt tgagcccgag gacgggcact cggagcctgt gcacacagtg
     3481 gtgttccagg gagcagcagg catcgggaaa accatcctag ccaggaagat tatgttggac
     3541 tgggcactgg gaaagctctt caaagacaaa tttgactatt tgttctttat ccactgccga
     3601 gaggtgagcc tcaggacgcc aaggagtcta gcagacctga ttgtcagctg ctggcctgac
     3661 ccaaacccac cagtgtgcaa gatcctgcgc aagccttcca ggatcctctt cctcatggat
     3721 ggctttgatg agctacaagg ggcctttgac gagcacattg gggaggtctg cacagactgg
     3781 caaaaggctg tgcggggaga cattctgcta agcagcctca tccgaaagaa actgctgccc
     3841 aaggcctctc tgctcataac gacgaggccg gtagccttgg agaaactgca gcatctcctg
     3901 gaccaccccc gccatgtgga gatcctaggt ttctctgagg ccaaaaggaa ggagtatttc
     3961 tttaagtatt tctccaacga gctgcaggcc cgggaggcct tcaggctgat ccaagagaat
     4021 gaggtcctct ttaccatgtg cttcatcccc ctggtctgct ggattgtgtg cacggggcta
     4081 aagcaacaga tggagaccgg gaagagcctg gcccagacct ccaagaccac tacggccgtc
     4141 tacgtcttct tcctttccag cctgctgcaa tcccgggggg gcattgagga gcatctcttc
     4201 tctgactacc tacaggggct ctgttcactg gctgcggatg gaatttggaa ccagaaaatc
     4261 ctatttgagg agtgtgatct gcggaagcac ggcctgcaga agactgacgt ctccgctttc
     4321 ctgaggatga acgtgttcca gaaggaagtg gactgcgaga gattctacag cttcagccac
     4381 atgactttcc aggagttctt cgctgctatg tactatttgc tggaagagga ggcagagggg
     4441 gagaccgtga ggaaaggacc aggaggttgt tcagatcttc tgaaccgaga cgtgaaggtc
     4501 ctactagaaa attacggcaa gtttgaaaaa ggctatctga tttttgttgt ccgattcctc
     4561 tttggccttg taaaccagga gagaacctct tatttggaga agaaactaag ttgcaagatc
     4621 tctcagcaag tcagactgga actactgaag tggattgaag tgaaagccaa ggccaagaag
     4681 ctgcagtggc agcccagcca actggaactg ttctactgcc tgtacgagat gcaggaggaa
     4741 gactttgtgc agagtgccat ggaccacttt cccaaaattg agatcaacct ctctaccaga
     4801 atggaccacg tggtttcctc cttttgtatt aagaactgtc atagggtcaa aacgctttcc
     4861 ctgggttttt ttcacaactc gcccaaggag gaagaagaag agaggagagg aggtcgaccc
     4921 ttggaccagg ttcagtgtgt tttcccagac actcatgttg cctgttcttc cagactggtg
     4981 aactgctgcc tcacttctag cttctgccgt ggtctcttct caagtctaag caccaaccgg
     5041 agcctcactg aactggacct cagtgacaat actctgggag acccgggcat gagggtgctg
     5101 tgtgaggcac tccagcaccc aggctgtaac attcagagac tgtggttggg gcgctgcgga
     5161 ctgtcccatc aatgctgctt cgacatctcc tctgtcctga gcagcagcca gaagctggtg
     5221 gagctggacc tcagtgacaa tgccctgggg gactttggaa tcagattgct gtgtgtggga
     5281 ctgaagcacc tgctctgcaa cctccagaaa ctgtggttgg tgagctgctg tctcacatcc
     5341 gcgtgttgtc aggatctcgc attggttctg agctccaacc attctctgac cagactgtac
     5401 attggagaaa atgccttggg agactcagga gtccaagttt tgtgtgaaaa gatgaaggac
     5461 ccacagtgta acttgcagaa gctggggttg gtgaattccg gccttacttc aatctgttgt
     5521 tcagctctga cctctgtgct caaaaccaac cagaacttca cacacctcta tctacgaagc
     5581 aatgcccttg gagacacagg actcaggctc ctctgtgagg ggcttctgca cccggactgt
     5641 aaactacaga tgctggaatt agacaactgc agcctcacct cacacagctg ctggaatctc
     5701 tccacaattc tgacccacaa ccacagcctt cggaagctga acctgggcaa caatgatctt
     5761 ggcgatctgt gcgtggtgac cctctgtgag gtgctgaaac agcagggctg cctcctgcag
     5821 agcctacagt tgggtgaaat gtacttaaat cgtgaaacaa aacgtgcctt agaagcgctc
     5881 caggaagaaa agcctgagct gactatagtc ttcgagattt cctgggaaca aaaactcatc
     5941 tcagaagagg atctgtgagg gcccgggatc ccgcgactct agataattct accgggtagg
     6001 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
     6061 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     6121 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     6181 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     6241 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     6301 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     6361 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     6421 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     6481 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc
     6541 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6601 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6661 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     6721 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     6781 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6841 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6901 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6961 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     7021 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     7081 acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga
     7141 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7201 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7261 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7321 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7381 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7441 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7501 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     7561 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7621 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7681 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac
     7741 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     7801 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     7861 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga
     7921 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     7981 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     8041 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     8101 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     8161 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     8221 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
     8281 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
     8341 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
     8401 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8461 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8521 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     8581 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta
     8641 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     8701 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8761 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8821 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8881 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8941 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9001 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9061 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9121 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9181 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9241 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9301 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9361 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9421 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9481 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9541 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9601 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9661 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9721 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9781 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9841 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9901 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9961 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10021 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10081 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10141 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10201 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10261 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10321 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10381 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10441 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10501 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10561 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10621 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10681 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10741 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10801 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac
    10861 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    10921 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    10981 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    11041 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    11101 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
    11161 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
