LOCUS V028475 12532 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V028475 VERSION V028475 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..7228 /label="Ncoa2(NM_008678)" /note="Ncoa2(NM_008678)" /gene="Ncoa2" CDS 7235..7300 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 7320..7819 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 7840..8439 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 8453..9041 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 9248..9881 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(10010..10026) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 10034..10050 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(10058..10088) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 10103..10124 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(10412..11000) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(11171..12031) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(12032..12136) /gene="bla" /label="AmpR promoter" polyA_signal 12184..12318 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgagtgg gatgggagaa aacacctctg acccgtccag 2881 ggcagagacc agaaaacgca aggaatgtcc cgaccagctc ggacccagcc ccaaaaggag 2941 cactgagaaa cggaaccgcg agcaggagaa taagtacata gaggagctgg ccgagctgat 3001 cttcgcaaac tttaatgata ttgacaactt caacttcaaa cctgacaaat gtgccatcct 3061 aaaagaaact gtgaagcaga tccgccagat caaagagcaa gagaaagcag cagctgccaa 3121 catagatgaa gtgcagaagt cagatgtgtc gtccacgggg cagggtgtca tcgacaagga 3181 tgcactgggg cccatgatgc ttgaggccct cgatgggttc ttcttcgttg tgaacctgga 3241 aggcagtgtg gtgttcgtgt cagagaatgt gacacagtat ctacggtata accaagaaga 3301 gctgatgaac aagagtgtct acagcatcct gcatgtcggg gaccacactg aatttgtcaa 3361 gaacctgctg ccaaagtcca tggtgaatgg aggatcctgg tctggagaac ctcccaggcg 3421 gagcagccat accttcaact gtcgcatgct ggtgaagcct ttgccagatt cagaagagga 3481 aggccatgat agccaggaag cccatcagaa atacgaggcg atgcagtgct tcgctgtgtc 3541 tcagcccaag tccatcaaag aggaaggcga agatttgcag tcctgcttga tttgtgtggc 3601 acgaagagtc cccatgaagg aaagaccaac tcttccctca tcagaaagct ttaccacccg 3661 ccaggacctc caaggcaaga tcacttcact ggacactagc accatgagag ccgccatgaa 3721 gccgggctgg gaagatctgg taagaagatg cattcagaag ttccacacac agcatgaagg 3781 ggagtctcta tcatatgcca agaggcatca ccatgaagtt ctgagacaag ggttggcgtt 3841 cagtcagatc tatcgttttt ctttgtctga tggcactctc gttgctgcac aaaccaagag 3901 caaactcatc cgttctcaga ctactaatga gcctcagctt gtaatatctt tacacatgct 3961 tcacagagag cagaatgtat gtgtaatgaa tccggatctg actggacaag cgatggggaa 4021 gccattgaat ccaattagct ctagcagccc tgcccaccag gccctgtgca gtgggaaccc 4081 aggtcaggac atgaccctcg gtagcaatat aaattttccc atgaatggcc caaaggaaca 4141 aatgggcatg cctatgggca ggtttggtgg ttctgggggc atgaaccatg tgtcaggcat 4201 gcaagcaacc actcctcagg gtagtaacta tgcactcaaa atgaacagtc cctcgcaaag 4261 cagccccggc atgaacccgg ggcaagccag ctccgtgctc tccccaaggc agcgcatgag 4321 ccccggcgtg gctggcagtc ctcgcatccc acccagtcag ttttcccctg caggaagctt 4381 gcattcccct gtgggagttt gcagcagcac aggaaatagc catagttata ccaacagttc 4441 cctcaatgca ctgcaagccc tcagcgaggg ccatggggtc tcactcgggt cctcgctggc 4501 ttcaccggac ctaaaaatgg gcaatttgca aaactcccca gttaatatga atcctccccc 4561 actcagcaag atgggaagct tggactccaa agactgtttt ggactttatg gggagccctc 4621 agaaggtaca actggacaag cagaggccag ctgccatcct gaagaacaaa aggggcccaa 4681 tgattccagc atgccccagg cggccagcgg ggacagggct gagggacaca gccggctgca 4741 tgacagcaaa gggcagacca aactcctgca gctgctgacc accaagtccg accagatgga 4801 gccttcaccc ttgcccagct ccttgtcgga cacaaacaag gactcaacag ggagcttgcc 4861 tgggcctggg tccacgcatg gcacctcgct caaggagaag cataagattt tgcacagact 4921 cttacaggac agcagttccc ctgtggactt ggccaagctg acagcagaag ccacaggcaa 4981 agagctgagc caggagtcca gcagcacagc tcctgggtcg gaagtgactg tcaaacagga 5041 gccagcgagc cccaagaaga aagagaatgc actactgcgc tatttgctcg acaaagatga 5101 tactaaagat attggtttac cggaaataac ccccaaactc gagcgactgg acagtaagac 5161 agatcctgcc agtaacacaa agttaattgc tatgaaaact gtgaaggagg aggtgagctt 5221 tgagcccagt gaccagcctg gcagcgagct ggacaacttg gaagagattt tggatgattt 5281 gcagaacagt cagttaccac agcttttccc agacacaagg ccaggagctc ctactgggtc 5341 agttgacaag caagccatca tcaatgacct catgcaactc acagctgaca gcagtcccgt 5401 cccacctgcc ggagcccaga aggcagcact gcgcatgtca cagagcactt ttaataaccc 5461 acgaccaggg caactgggca ggttattgcc aaaccagaac ttaccacttg acatcacttt 5521 gcaaagccca actggtgctg gacctttccc accaatcaga aacagtagcc cctactcagt 5581 gatacctcag ccaggaatga tgggtaacca agggatgcta ggaagccaag gaaacttagg 5641 gaacaatagc acaggaatga ttggcagcag cacttcccgg cccagcatgc cttctgggga 5701 atgggcacca cagagtccag ctgtgagagt cacttgtgct gctaccactg gtgccatgaa 5761 ccgaccagtc caaggaggca tgattcggaa cccaacagcc agcatcccca tgcgagccaa 5821 cagccagcct ggccaaagac agatgcttca gtctcaggtc atgaacatag gcccttctga 5881 gttagagatg aacatgggag gacctcagta taatcaacag caggcccctc cgaaccaaac 5941 tgccccgtgg cctgagagca tcctgcctat agaccaggca tcgtttgcca gccagaacag 6001 gcagcccttc ggcagctccc ctgatgacct gctgtgtcca catcctgcag cagagtcgcc 6061 aagcgatgag ggcgctcttc ttgaccagct gtatctggcc ttgcggaact tcgatggcct 6121 tgaggagatt gatagagctc tggggatacc agaactggtc agccagagcc aagctgtgga 6181 tgcagagcag ttctcaagtc aggagtccag cataatgctg gagcagaagc cccccgtttt 6241 cccacagcag tacgcatctc aggcacaaat ggcccagggt ggctataatc ccatgcaaga 6301 tccaaacttt cacaccatgg gacagcggcc aaattacacc acactccgta tgcagccacg 6361 gccaggcctc aggcccacag gcattgtgca gaaccagcca aaccaactga gacttcagct 6421 tcagcaccgc ctccaagcac agcagaatcg ccagccgctt atgaatcaga tcagcagtgt 6481 ttccaatgtg aacctgactc tgaggcctgg agtgcccact caggctccta ttaatgcaca 6541 gatgctggcc cagaggcaga gggaaatcct caaccaacat cttcggcaga gacagatgca 6601 gcagcaggtg cagcagcgga ctttgatgat gagaggacag ggcttgaatg tgaccccaag 6661 catggtggct cccgctggcc taccagcagc catgagcaat ccccggatcc cccaggccaa 6721 tgcccagcag ttcccatttc ctccgaacta cggaataagt caacaacctg atcctggctt 6781 tactggggct acaactcccc agagtcctct aatgtctccc cggatggcac atactcagag 6841 tcccatgatg cagcagtctc aagccaaccc agcctaccag cccacctcag acatgaatgg 6901 atgggcacag gggagcatgg gtggaaacag catgttctca cagcagtccc caccacactt 6961 tgggcaacaa gcaaacacca gcatgtatag taacaacatg aacatcagtg tgtcgatggc 7021 aaccaacacg ggtggcttga gcagcatgaa ccagatgaca ggccagatga gcatgacctc 7081 agtgacctcc gtgcctacgt caggactgcc ctccatgggt cccgagcagg tcaatgaccc 7141 tgctctgagg ggaggcaacc ttttcccaaa ccaactgcct ggaatggaca tgatcaagca 7201 ggagggagat gcatctcgga aatactgcgg atccgactac aaagaccatg acggtgatta 7261 taaagatcat gacatcgact acaaggatga cgatgacaag tagtctagat aattctaccg 7321 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 7381 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 7441 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 7501 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 7561 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 7621 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 7681 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 7741 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 7801 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg 7861 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 7921 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 7981 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 8041 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 8101 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 8161 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 8221 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 8281 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 8341 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 8401 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc 8461 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 8521 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 8581 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 8641 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 8701 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 8761 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 8821 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 8881 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 8941 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 9001 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt 9061 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat 9121 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta 9181 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 9241 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc 9301 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga 9361 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa 9421 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat 9481 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg 9541 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga 9601 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc 9661 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca 9721 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 9781 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 9841 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 9901 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca 9961 ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 10021 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 10081 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 10141 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 10201 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 10261 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 10321 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 10381 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 10441 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 10501 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 10561 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 10621 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 10681 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 10741 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 10801 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 10861 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 10921 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 10981 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 11041 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 11101 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 11161 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 11221 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 11281 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 11341 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 11401 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 11461 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 11521 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 11581 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 11641 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 11701 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 11761 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 11821 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 11881 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 11941 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 12001 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 12061 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 12121 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 12181 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 12241 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 12301 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc 12361 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat 12421 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta 12481 gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt //