LOCUS       V019332                10600 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019332
VERSION     V019332
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..3559
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3629..5368
                     /label="METTL3(NM_019852)"
                     /note="METTL3(NM_019852)"
                     /gene="METTL3"
     promoter        5388..5887
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5908..6507
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6521..7109
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7316..7949
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8078..8094)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8102..8118
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8126..8156)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8171..8192
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8480..9068)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9239..10099)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10100..10204)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10252..10386
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     2881 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2941 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     3001 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     3061 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     3121 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     3181 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     3241 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     3301 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     3361 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     3421 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     3481 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     3541 catggacgag ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg
     3601 gggaagcgga ggcggaggga gtggatccat gtcggacacg tggagctcta tccaggccca
     3661 caagaagcag ctggactctc tgcgggagag gctgcagcgg aggcggaagc aggactcggg
     3721 gcacttggat ctacggaatc cagaggcagc attgtctcca accttccgta gtgacagccc
     3781 agtgcctact gcacccacct ctggtggccc taagcccagc acagcttcag cagttcctga
     3841 attagctaca gatcctgagt tagagaagaa gttgctacac cacctctctg atctggcctt
     3901 aacattgccc actgatgctg tgtccatctg tcttgccatc tccacgccag atgctcctgc
     3961 cactcaagat ggggtagaaa gcctcctgca gaagtttgca gctcaggagt tgattgaggt
     4021 aaagcgaggt ctcctacaag atgatgcaca tcctactctt gtaacctatg ctgaccattc
     4081 caagctctct gccatgatgg gtgctgtggc agaaaagaag ggccctgggg aggtagcagg
     4141 gactgtcaca gggcagaagc ggcgtgcaga acaggactcg actacagtag ctgcctttgc
     4201 cagttcgtta gtctctggtc tgaactcttc agcatcggaa ccagcaaagg agccagccaa
     4261 gaaatcaagg aaacatgctg cctcagatgt tgatctggag atagagagcc ttctgaacca
     4321 acagtccact aaggaacaac agagcaagaa ggtcagtcag gagatcctag agctattaaa
     4381 tactacaaca gccaaggaac aatccattgt tgaaaaattt cgctctcgag gtcgggccca
     4441 agtgcaagaa ttctgtgact atggaaccaa ggaggagtgc atgaaagcca gtgatgctga
     4501 tcgaccctgt cgcaagctgc acttcagacg aattatcaat aaacacactg atgagtcttt
     4561 aggtgactgc tctttcctta atacatgttt ccacatggat acctgcaagt atgttcacta
     4621 tgaaattgat gcttgcatgg attctgaggc ccctggcagc aaagaccaca cgccaagcca
     4681 ggagcttgct cttacacaga gtgtcggagg tgattccagt gcagaccgac tcttcccacc
     4741 tcagtggatc tgttgtgata tccgctacct ggacgtcagt atcttgggca agtttgcagt
     4801 tgtgatggct gacccaccct gggatattca catggaactg ccctatggga ccctgacaga
     4861 tgatgagatg cgcaggctca acatacccgt actacaggat gatggctttc tcttcctctg
     4921 ggtcacaggc agggccatgg agttggggag agaatgtcta aacctctggg ggtatgaacg
     4981 ggtagatgaa attatttggg tgaagacaaa tcaactgcaa cgcatcattc ggacaggccg
     5041 tacaggtcac tggttgaacc atgggaagga acactgcttg gttggtgtca aaggaaatcc
     5101 ccaaggcttc aaccagggtc tggattgtga tgtgatcgta gctgaggttc gttccaccag
     5161 tcataaacca gatgaaatct atggcatgat tgaaagacta tctcctggca ctcgcaagat
     5221 tgagttattt ggacgaccac acaatgtgca acccaactgg atcacccttg gaaaccaact
     5281 ggatgggatc cacctactag acccagatgt ggttgcacgg ttcaagcaaa ggtacccaga
     5341 tggtatcatc tctaaaccta agaatttata gtctagataa ttctaccggg taggggaggc
     5401 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     5461 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     5521 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     5581 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     5641 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     5701 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     5761 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     5821 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     5881 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     5941 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6001 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6061 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6121 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6181 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6241 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6301 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6361 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     6421 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     6481 tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa
     6541 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6601 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6661 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6721 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6781 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6841 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6901 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     6961 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7021 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7081 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa
     7141 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     7201 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     7261 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa
     7321 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     7381 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
     7441 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
     7501 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     7561 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
     7621 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
     7681 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
     7741 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     7801 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7861 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7921 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     7981 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt
     8041 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     8101 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8161 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8221 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8281 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8341 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8401 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8461 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8521 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8581 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8641 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8701 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8761 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8821 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8881 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8941 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9001 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9061 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9121 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9181 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9241 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9301 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9361 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9421 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9481 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9541 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9601 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9661 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9721 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9781 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9841 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9901 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9961 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10021 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10081 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10141 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10201 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt
    10261 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    10321 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    10381 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
    10441 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
    10501 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa
    10561 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
