LOCUS V018784 9225 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018784 VERSION V018784 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2830..2856 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2857..3993 /label="MAPK3(NM_002746)" /note="MAPK3(NM_002746)" /gene="MAPK3" promoter 4013..4512 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 4533..5132 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5146..5734 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5941..6574 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6703..6719) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6727..6743 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6751..6781) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6796..6817 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7105..7693) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7864..8724) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8725..8829) /gene="bla" /label="AmpR promoter" polyA_signal 8877..9011 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 gccaccatgt acccttatga cgtccccgat tacgccatgg cggcggcggc ggctcagggg 2881 ggcgggggcg gggagccccg tagaaccgag ggggtcggcc cgggggtccc gggggaggtg 2941 gagatggtga aggggcagcc gttcgacgtg ggcccgcgct acacgcagtt gcagtacatc 3001 ggcgagggcg cgtacggcat ggtcagctcg gcctatgacc acgtgcgcaa gactcgcgtg 3061 gccatcaaga agatcagccc cttcgaacat cagacctact gccagcgcac gctccgggag 3121 atccagatcc tgctgcgctt ccgccatgag aatgtcatcg gcatccgaga cattctgcgg 3181 gcgtccaccc tggaagccat gagagatgtc tacattgtgc aggacctgat ggagactgac 3241 ctgtacaagt tgctgaaaag ccagcagctg agcaatgacc atatctgcta cttcctctac 3301 cagatcctgc ggggcctcaa gtacatccac tccgccaacg tgctccaccg agatctaaag 3361 ccctccaacc tgctcatcaa caccacctgc gaccttaaga tttgtgattt cggcctggcc 3421 cggattgccg atcctgagca tgaccacacc ggcttcctga cggagtatgt ggctacgcgc 3481 tggtaccggg ccccagagat catgctgaac tccaagggct ataccaagtc catcgacatc 3541 tggtctgtgg gctgcattct ggctgagatg ctctctaacc ggcccatctt ccctggcaag 3601 cactacctgg atcagctcaa ccacattctg ggcatcctgg gctccccatc ccaggaggac 3661 ctgaattgta tcatcaacat gaaggcccga aactacctac agtctctgcc ctccaagacc 3721 aaggtggctt gggccaagct tttccccaag tcagactcca aagcccttga cctgctggac 3781 cggatgttaa cctttaaccc caataaacgg atcacagtgg aggaagcgct ggctcacccc 3841 tacctggagc agtactatga cccgacggat gagccagtgg ccgaggagcc cttcaccttc 3901 gccatggagc tggatgacct acctaaggag cggctgaagg agctcatctt ccaggagaca 3961 gcacgcttcc agcccggagt gctggaggcc ccctagtcta gataattcta ccgggtaggg 4021 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg 4081 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg 4141 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc 4201 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact 4261 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc 4321 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc 4381 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc 4441 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct 4501 ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc 4561 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 4621 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 4681 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 4741 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 4801 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 4861 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 4921 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 4981 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 5041 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 5101 cctggtgcat gacccgcaag cccggtgcct gaccgcgtct ggaacaatca acctctggat 5161 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 5221 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 5281 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 5341 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 5401 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 5461 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 5521 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc 5581 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 5641 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 5701 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc 5761 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct 5821 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa 5881 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac 5941 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca 6001 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 6061 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 6121 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 6181 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 6241 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 6301 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 6361 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 6421 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 6481 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 6541 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 6601 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag 6661 cgttttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 6721 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 6781 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 6841 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 6901 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 6961 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 7021 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 7081 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 7141 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 7201 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 7261 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7321 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7381 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7441 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7501 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 7561 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7621 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 7681 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 7741 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 7801 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 7861 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 7921 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 7981 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 8041 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 8101 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 8161 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 8221 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8281 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8341 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8401 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8461 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 8521 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8581 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 8641 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 8701 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 8761 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 8821 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact 8881 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 8941 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 9001 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat 9061 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg 9121 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttt 9181 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt //