LOCUS       V021544                10961 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021544
VERSION     V021544
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..5687
                     /label="MAP3K10(NM_002446)"
                     /note="MAP3K10(NM_002446)"
                     /gene="MAP3K10"
     CDS             5694..5717
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     promoter        5749..6248
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6269..6868
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6882..7470
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7677..8310
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8439..8455)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8463..8479
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8487..8517)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8532..8553
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8841..9429)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9600..10460)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10461..10565)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10613..10747
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgga ggaggaggag ggggcggtgg ccaaggagtg gggcacgacc cccgcggggc
     2881 ccgtctggac cgcggtgttc gactacgagg cggcgggcga cgaggagctg accctgcgga
     2941 ggggcgatcg cgtccaggtg ctttcccaag actgtgcggt gtccggcgac gagggctggt
     3001 ggaccgggca gctccccagc ggccgcgtgg gcgtcttccc cagcaactac gtggcccccg
     3061 gcgcccccgc tgcacccgcg ggcctccagc tgccccagga gatccccttc cacgagctgc
     3121 agctagagga gatcatcggt gtggggggct ttggcaaggt ctatcgggcc ctgtggcgtg
     3181 gcgaggaggt ggcagtcaag gccgcccggc tggaccctga gaaggacccg gcagtgacag
     3241 cggagcaggt gtgccaggaa gcccggctct ttggagccct gcagcacccc aacataattg
     3301 cccttagggg cgcctgcctc aaccccccac acctctgcct agtgatggag tatgcccggg
     3361 gtggtgcact gagcagggtg ctggcaggtc gccgggtgcc acctcacgtg ctggtcaact
     3421 gggctgtgca ggtggcccgg ggcatgaact acctacacaa tgatgcccct gtgcccatca
     3481 tccaccggga cctcaagtcc atcaacatcc tgatcctgga ggccatcgag aaccacaacc
     3541 tcgcagacac ggtgctcaag atcacggact tcggcctcgc ccgcgagtgg cacaagacca
     3601 ccaagatgag cgctgcgggg acctacgcct ggatggcgcc ggaggttatc cgtctctccc
     3661 tcttctccaa aagcagtgat gtctggagct tcggggtgct gctgtgggag ctgctgacgg
     3721 gggaggtccc ctaccgtgag atcgacgcct tggccgtggc gtatggcgtg gctatgaata
     3781 agctgacgct gcccattccc tccacgtgcc ccgagccctt tgcccgcctc ctggaggaat
     3841 gctgggaccc agacccccac gggcggccag atttcggtag catcttgaag cggcttgaag
     3901 tcatcgaaca gtcagccctg ttccagatgc cactggagtc cttccactcg ctgcaggaag
     3961 actggaagct ggagattcag cacatgtttg atgaccttcg gaccaaggag aaggagcttc
     4021 ggagccgtga ggaggagctg ctgcgggcgg cacaggagca gcgcttccag gaggagcagc
     4081 tgcggcggcg ggagcaggag ctggcagaac gtgagatgga catcgtggaa cgggagctgc
     4141 acctgctcat gtgccagctg agccaggaga agccccgggt ccgcaagcgc aagggcaact
     4201 tcaagcgcag ccgcctgctc aagctgcggg aaggcggcag ccacatcagc ctgccctctg
     4261 gctttgagca taagatcaca gtccaggcct ctccaactct ggataagcgg aaaggatccg
     4321 atggggccag cccccctgca agccccagca tcatcccccg gctgagggcc attcgcctga
     4381 ctcccgtgga ctgtggtggc agcagcagtg gcagcagcag tggaggaagt gggacatgga
     4441 gccgcggtgg gcccccaaag aaggaagaac tggtcggggg caagaagaag ggacgaacgt
     4501 gggggcccag ctccaccctg cagaaggagc gggtgggagg agaggagagg ctgaaggggc
     4561 tgggggaagg aagcaaacag tggtcatcaa gtgcccccaa cctgggcaag tcccccaaac
     4621 acacacccat cgcccctggc tttgccagcc tcaatgagat ggaggagttc gcggaggcag
     4681 aggatggagg cagcagcgtg cccccttccc cctactcgac cccgtcctac ctctcagtgc
     4741 cactgcctgc cgagccctcc ccgggggcgc gggcgccgtg ggagccgacg ccgtccgcgc
     4801 cccccgctcg gtggggacac ggcgcccggc ggcgctgcga cctggcgctg ctaggctgcg
     4861 ccacgctgct gggggctgtg ggcctgggcg ccgacgtggc cgaggcgcgc gcggccgacg
     4921 gtgaggagca gcggcgctgg ctcgacggcc tcttctttcc ccgcgccggc cgcttcccgc
     4981 ggggcctcag cccacccgcg cgtccccacg gccgccgcga agacgtgggc cccggcctgg
     5041 gcctggcgcc ctcggccacc ctcgtgtcgc tgtcgtccgt gtccgactgc aactccacgc
     5101 gttcactgct gcgctctgac agtgacgagg ccgcaccggc cgcgccctcc ccaccaccct
     5161 ccccgcccgc gcccacaccc acgccctcgc ccagcaccaa ccccctggtg gacctggagc
     5221 tggagagctt caagaaggac ccccgccagt cgctcacgcc cacccacgtc acggctgcat
     5281 gcgctgtgag ccgcgggcac cggcggacgc catcggacgg ggcgctgggg cagcgggggc
     5341 cgcccgagcc cgcgggccat ggccctggcc ctcgtgacct tctggacttc ccccgcctgc
     5401 ccgaccccca ggccctgttc ccagcccgcc gccggccccc tgagttccca ggccgcccca
     5461 ccaccctgac ctttgccccg agacctcggc cggctgccag tcgcccccgc ttggacccct
     5521 ggaaactggt ctccttcggc cggacactca ccatctcgcc tcccagcagg ccagacactc
     5581 cggagagccc tgggcccccc agcgtgcagc ccacactgct ggacatggac atggaggggc
     5641 agaaccaaga cagcacagtg cccctgtgcg gggcccacgg ctcccacgaa ttcgattaca
     5701 aggatgacga cgataagtga ggatcccgcg actctagata attctaccgg gtaggggagg
     5761 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     5821 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     5881 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     5941 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     6001 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     6061 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     6121 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     6181 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     6241 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     6301 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     6361 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     6421 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     6481 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     6541 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     6601 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     6661 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     6721 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     6781 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     6841 gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca
     6901 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6961 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7021 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7081 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7141 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7201 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7261 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     7321 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7381 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7441 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga
     7501 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     7561 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     7621 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga
     7681 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     7741 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     7801 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     7861 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     7921 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     7981 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     8041 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     8101 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     8161 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8221 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8281 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     8341 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt
     8401 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     8461 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8521 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8581 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8641 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8701 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8761 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8821 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8881 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8941 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9001 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9061 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9121 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9181 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9241 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9301 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9361 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9421 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9481 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9541 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9601 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9661 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9721 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9781 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9841 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9901 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9961 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10021 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10081 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10141 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10201 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10261 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10321 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10381 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10441 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10501 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10561 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt
    10621 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10681 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10741 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
    10801 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
    10861 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagtttttaa
    10921 agaaattgta tttgttaaat atgtactaca aacttagtag t
//
