LOCUS       V021257                12464 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021257
VERSION     V021257
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..7190
                     /label="MAGI2(NM_012301)"
                     /note="MAGI2(NM_012301)"
                     /gene="MAGI2"
     CDS             7197..7220
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     promoter        7252..7751
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             7772..8371
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8385..8973
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9180..9813
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9942..9958)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9966..9982
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9990..10020)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10035..10056
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10344..10932)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11103..11963)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11964..12068)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    12116..12250
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgtc caaaagcttg aaaaagaaaa gccactggac tagcaaagtc catgagagtg
     2881 tcattggcag gaacccggag ggccagctgg gctttgaact gaaggggggc gccgagaatg
     2941 gacagttccc ctacctgggg gaggtgaagc ccggcaaggt ggcctatgag agcggcagca
     3001 aattggtgtc ggaggagctg ctgctggagg tgaacgagac ccccgtggcg gggctcacca
     3061 tcagggacgt gctggccgtg atcaaacact gcaaggaccc cctccggctc aagtgtgtca
     3121 agcaaggagg aattgttgat aaagaccttc gtcactacct caacttacga tttcaaaagg
     3181 gttctgtgga ccatgagctt cagcaaatca ttcgtgacaa cctctacctc cgcacggtgc
     3241 catgcaccac aaggccacat aaggagggtg aggtccctgg agtggattat attttcatca
     3301 ctgttgaaga ttttatggaa ttggagaaaa gtggtgctct cctagaaagt gggacttatg
     3361 aagacaatta ctacggtacc ccaaagccgc cagcagaacc agcaccatta ttgttaaatg
     3421 taacagacca gatacttcca ggagccactc caagtgctga aggaaaacgg aagaggaata
     3481 aatcagtgag caacatggag aaagccagta tagagcctcc tgaggaggaa gaggaagaga
     3541 ggcctgtggt caatggaaat ggagtagtag taacaccaga atccagtgaa catgaagaca
     3601 aaagtgcagg tgcctcaggg gagatgccct cccagcctta tcctgcacca gtgtacagtc
     3661 agcctgagga gctgaaggag cagatggatg acacaaagcc aactaaacct gaagacaatg
     3721 aggaaccaga cccattgcct gataactggg aaatggccta tacagagaag ggcgaagtct
     3781 acttcattga ccataacaca aagacaacat catggctgga tccacgactt gcgaaaaagg
     3841 ctaaacctcc agaagagtgc aaagaaaatg agcttccata tggctgggaa aaaatcgatg
     3901 atcccattta tggcacttat tatgttgacc acataaatag aagaacacag tttgaaaatc
     3961 ctgtcctgga agcaaaaagg aagctacagc aacataacat gccccacaca gaacttggaa
     4021 caaagcccct gcaggcccca ggtttccgag aaaaaccact cttcacccgg gatgcatccc
     4081 agttgaaggg aacattcctc agcaccaccc taaaaaagag caacatgggc tttggattta
     4141 ccatcattgg tggagacgag cctgatgagt ttctgcaggt gaaaagtgtg attccggatg
     4201 ggcctgcagc acaggatgga aaaatggaaa caggtgatgt cattgtctat attaatgaag
     4261 tttgtgtcct tggacacact catgcagatg ttgtcaaact tttccagtct gttcctattg
     4321 gtcagagtgt caacctggtg ttgtgtcgtg gctacccttt gccctttgat cctgaagacc
     4381 ctgctaacag catggtgcca ccccttgcaa taatggagag gccacctcca gtgatggtca
     4441 atggaagaca caactatgaa acatatttgg agtacatttc tcggacctca cagtcagttc
     4501 cagatataac agatcggccg cctcattctc tgcactccat gccaactgat ggtcagctag
     4561 acggcacgta tccaccgccc gtccatgatg acaatgtgtc tatggcttca tctggggcca
     4621 cccaagctga acttatgacc ttaaccattg tgaaaggtgc ccagggcttc ggcttcacta
     4681 ttgccgacag tcctacagga cagcgggtga aacaaatact tgacattcag ggatgccctg
     4741 gcctgtgtga aggcgacctc attgttgaga tcaaccagca gaatgtacag aacctgagcc
     4801 atacagaagt agtggatata cttaaggact gtcccattgg aagtgaaact tctttgatta
     4861 tccatcgagg aggtttcttt tctccatgga aaactccaaa gcctataatg gaccgatggg
     4921 agaatcaagg cagtcctcaa acgagtttat ctgctccggc cataccgcag aacctgccct
     4981 tcccacctgc ccttcacagg agctcctttc ctgactcaac agaggccttt gacccacgga
     5041 agcctgatcc atatgagctc tacgagaaat ctagggccat ttatgaaagt aggcaacaag
     5101 tgccacccag gaccagtttt cgaatggatt cctctggtcc agattataag gaattggatg
     5161 ttcatcttcg gaggatggag tctggatttg gcttcagaat cctcggggga gatgagcctg
     5221 gacagcctat tttgattgga gctgtcattg ccatgggctc agccgacaga gatggccgcc
     5281 ttcacccagg agatgagctt gtgtatgttg atgggattcc agtagccggc aaaacccacc
     5341 gctatgtcat cgacctcatg caccacgcag cccgcaatgg gcaggtcaac ctcactgtga
     5401 gaagaaaggt gctatgtgga ggggagccct gcccagagaa cgggagaagt ccaggctctg
     5461 tatccaccca ccacagctct ccacgcagtg actacgcaac ctacaccaac agcaaccacg
     5521 ctgcccccag tagcaatgcc tctccccctg aaggcttcgc ctcccacagc ctgcagacca
     5581 gtgatgtggt cattcaccgc aaagagaatg agggcttcgg ctttgtcatc atcagctccc
     5641 tgaacaggcc tgagtctgga tccactataa ctgtgcccca taaaatcgga cgcatcattg
     5701 atgggagtcc tgcagatcgc tgtgcaaaac taaaagtggg agaccggatc ctagcagtga
     5761 atggccagtc tatcatcaac atgcctcacg ctgacatcgt gaagctcatc aaggatgcag
     5821 gtcttagtgt cacccttcgc atcattcctc aggaggagct caacagcccc acctcggcac
     5881 ccagctcaga gaagcagagt cccatggcgc agcagagtcc cctggcacag cagagtcccc
     5941 tggcccagcc aagcccagcc acccccaaca gccccatcgc ccagccagca ccacctcaac
     6001 cacttcagct gcaaggacac gaaaatagtt acaggtcgga agtgaaagca aggcaagatg
     6061 tgaaaccaga catccgacag cctccattca cagactacag gcagcccccg ctggattaca
     6121 ggcaaccccc aggaggggac taccagcagc ccccaccctt ggactacagg cagcctcccc
     6181 tactggacta caggcagcac tcccccgaca ccaggcagta ccctctgtcg gactacagac
     6241 aaccccagga ttttgattat ttcactgtgg acatggagaa aggagccaaa ggatttggat
     6301 tcagcattcg tggaggaagg gaatacaaaa tggatttgta tgtgttgaga ctggcagaag
     6361 atggaccagc aataaggaat gggaggatga gggtaggaga tcaaatcatt gaaatcaatg
     6421 gggaaagcac aagggacatg acacatgcca gagcaataga actcatcaaa tctggaggaa
     6481 gacgagtgag gctgctgctc aagagaggca cgggacaggt cccagaatat gacgaacccg
     6541 ccccctggag ttctcccgct gccgccgccc caggtctgcc ggaagtaggc gtctccctgg
     6601 acgacggcct cgctccattc tctccatcac atccagcccc accctccgac ccttcccacc
     6661 agataagccc aggcccaact tgggatatca aacgggaaca cgacgttagg aaaccaaagg
     6721 agctttcagc ctgcggccag aagaagcagc gcctcgggga gcagagggag cgctcggcga
     6781 gtccgcagag ggccgcgcgg ccgaggctcg aggaggcgcc cggcggccag gggcggcccg
     6841 aggccggcag gcccgcctcg gaggccaggg cgcccgggct cgcggcggca gacgcggcgg
     6901 acgcggcgcg ggcgggcggg aaggaggcgc cccgtgcggc ggcgggctcc gagctctgcc
     6961 ggcgcgaagg cccgggggct gcgccggcgt ttgccggccc gggcggcggc ggcagcggcg
     7021 cgctggaggc cgagggcagg gcgggtgcgc gcgcgggtcc ccggccgggc ccgcgacccc
     7081 cggggggcgc cccggcgcgc aaggccgccg tcgcgccggg gccctggaag gtgccgggtt
     7141 ctgacaagct gcccagcgtc ctcaaacccg gcgcctcggc cgccagcaga gaattcgatt
     7201 acaaggatga cgacgataag tgaggatccc gcgactctag ataattctac cgggtagggg
     7261 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     7321 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     7381 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     7441 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     7501 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     7561 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     7621 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     7681 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     7741 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct
     7801 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     7861 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     7921 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     7981 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     8041 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     8101 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     8161 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     8221 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     8281 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     8341 ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa cctctggatt
     8401 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     8461 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     8521 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     8581 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     8641 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     8701 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     8761 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     8821 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     8881 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     8941 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct
     9001 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     9061 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     9121 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact
     9181 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     9241 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     9301 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     9361 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     9421 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     9481 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     9541 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     9601 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     9661 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     9721 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     9781 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     9841 agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc
     9901 gttttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     9961 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    10021 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    10081 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    10141 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    10201 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    10261 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    10321 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    10381 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    10441 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    10501 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    10561 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    10621 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    10681 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    10741 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    10801 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    10861 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    10921 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    10981 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    11041 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    11101 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    11161 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    11221 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    11281 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    11341 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    11401 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    11461 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    11521 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    11581 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    11641 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    11701 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    11761 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    11821 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    11881 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    11941 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    12001 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    12061 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt
    12121 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    12181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    12241 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
    12301 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
    12361 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt
    12421 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
