LOCUS       V023946                11891 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023946
VERSION     V023946
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..5900
                     /label="Lnpep(NM_172827)"
                     /note="Lnpep(NM_172827)"
                     /gene="Lnpep"
     CDS             5940..6650
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="photostable monomeric orange derivative of DsRed
                     fluorescent protein (Shaner et al., 2008)"
                     /transl_table=1
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mOrange2"
     promoter        6679..7178
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             7199..7798
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7812..8400
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8607..9240
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9369..9385)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9393..9409
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9417..9447)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9462..9483
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9771..10359)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10530..11390)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11391..11495)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11543..11677
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgga gtcctttacc aatgatcggc ttcagcttcc aaggaatatg atcgaaaaca
     2881 gcatgtttga agaagagcca gatgtggtag atttagccaa agaaccttgt ttacatcctc
     2941 tggaacccga tgaagtggaa tatgagcccc gaggttcgag gcttctggtg cgaggtcttg
     3001 gtgagcatga gatggacgag gatgaagagg attatgagtc ctctgcgaag ctgctgggca
     3061 tgtccttcat gaacagaagc tcaggcctgc ggaacagtgc agcaggctac aggcagagtc
     3121 cagatgggac ttgttcatta ccctctgcca ggaccttagt gatctgtgtt tttgtcattg
     3181 tggttgcggt ctctgtaatc atggtgattt atctactgcc tagatgtacc tttaccaaag
     3241 aaggctgcca caaaacaaac cagtcagcag aactcatcca gccagttgct acaaacggga
     3301 aagtgttccc atgggctcaa attaggcttc ccactgccat tattcctcta tgctatgaac
     3361 ttagcctaca tccaaaccta acctcaatga cattcagggg atctgtgaca atttcacttc
     3421 aggctcttca agacacacgg gatatcattc tccatagcac aggacataat atttcaagag
     3481 tgacatttat gtcagcagtt tcaagtcaag aaaaacaagt tgaaattctg gaatatccat
     3541 atcatgaaca aatcgccgtt gttgccccgg aacctcttct aacaggacac aattataccc
     3601 tgaagataga gtattcagca aatatatcta actcttatta tgggttttat ggcatcacct
     3661 acacagataa aagtaatgag aaaaagtact ttgcagcaac tcagtttgaa cctctggcag
     3721 caagatctgc ttttccttgt tttgatgaac cagcatttaa ggccacattt atcatcaaga
     3781 tcacaaggaa tgagcaccat actgcgttat caaatatgcc taagaagtca tcggtccctg
     3841 cagaagaagg acttattcaa gatgagtttt ctgaaagtgt gaaaatgagc acatacctgg
     3901 ttgctttcat tgtaggggag atgaggaacc tgagtcagga tgtaaatgga actctggttt
     3961 ctgtgtatgc tgtaccagaa aaaattggtc aagttcacca tgccttggac acaactataa
     4021 agcttcttga gttttatcaa acttactttg aaattcagta cccacttaag aaattggatt
     4081 tggtggccat tcctgacttt gaagcaggag caatggaaaa ctggggcctg cttacattcc
     4141 gagaagagac tcttctgtat gacaatgcaa cttcttcagt agcagacaga aaactggtca
     4201 ctaaaatcat tgctcacgaa ctggcacatc agtggtttgg aaatctggtt acaatgcagt
     4261 ggtggaatga cctgtggcta aatgaaggct ttgccacttt catggagtat ttctctgtgg
     4321 aaaaaatatt caaagagctc aacagttatg aagacttctt agatgctcga tttaaaacca
     4381 tgaggaaaga ttccttgaat tcgtctcatc caatatcatc atctgttcag tcttcggaac
     4441 aaatagaaga aatgtttgat tctctctcct attttaaggg agcttctctc ttgttgatgc
     4501 tgaaaagtta ccttagtgaa gatgtatttc ggcatgctgt cattctttac ctgcacaatc
     4561 acagctatgc agctatccaa agtgatgatc tctgggacag cttcaatgag gtcacagaca
     4621 aaactctaga tgtaaagaaa atgatgaaaa cctggaccct acagaaagga ttcccgttag
     4681 tgacagtcca gaggaagggg actgagcttc ttctacaaca ggaaagattt tttctacgca
     4741 tgcaaccaga aagtcagcct tcagatacaa gccacctttg gcatattcca atatcctatg
     4801 tcactgatgg aagaaactat tcagaatatc gatcagtttc actactggac aagaaatcag
     4861 atgtcatcaa tcttacagaa caagtgcagt gggtcaaagt caattcaaac atgactggct
     4921 attacattgt tcactatgct catgatgact ggacagctct aatcaatcag ttaaaaagaa
     4981 acccttatgt tctgagtgac aaagaccgag ccaacctgat caataacatc tttgaacttg
     5041 caggtcttgg caaagtgcct cttcggatgg catttgattt gattgactat cttaaaaatg
     5101 agacccacac tgcaccgatc actgaagccc tgttccagac aaaccttatc tataatctcc
     5161 tagaaaaact ggggcacatg gacctgtcct caagattggt ggccagagta cataaattgc
     5221 tccagaacca aatccagcag cagacttgga cagatgaagg cacaccatcc atgcgagagc
     5281 ttcggtcagc cttgctggaa tttgcctgtg cccacagcct agagaactgt accactatgg
     5341 ccacaaatct gtttgacagt tggatggcat caaatggaac tcagagcctg cctactgatg
     5401 tcatggtcac tgtgttcaag gtgggagcga gaaccgagaa aggctggttg ttcctcttta
     5461 gcatgtattc ctccatgggc tctgaagcag aaaagaataa aattcttgaa gctcttgcca
     5521 gctcagagga tgtacataaa ctttactggt taatgaaaag tagccttgat ggtgacatca
     5581 tccgaacaca gaagttgtca cttatcatta gaacagtggg cagacatttt cctggacact
     5641 tgctggcttg ggattttgtt aaagaaaact ggaataagct tgtacataag ttccatctgg
     5701 gctcctatac catacaaagt attgttgctg gatctactca cttattttca acaaagacac
     5761 atttatctga ggtccaggca ttctttgaaa atcagtcaga ggcaaccttg aagcttcggt
     5821 gtgttcagga ggctttggaa gtgattcagc tgaatatcca gtggatggtc aggaatctga
     5881 aaacgctctc ccagtggctg gcggccgcag gtggaggcgg aagcggcggt ggaggttcta
     5941 tggtgagcaa gggcgaggag aataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     6001 tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     6061 gcccctacga gggctttcag accgctaagc tgaaggtgac caagggtggc cccctgccct
     6121 tcgcctggga catcctgtcc cctcatttca cctacggctc caaggcctac gtgaagcacc
     6181 ccgccgacat ccccgactac ttcaagctgt ccttccccga gggcttcaag tgggagcgcg
     6241 tgatgaacta cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     6301 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtga
     6361 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggtg
     6421 ccctgaaggg caagatcaag atgaggctga agctgaagga cggcggccac tacacctccg
     6481 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacatcgtcg
     6541 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     6601 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa ggatcccgcg
     6661 actctagata attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     6721 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     6781 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     6841 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     6901 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     6961 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     7021 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     7081 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     7141 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     7201 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     7261 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     7321 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     7381 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     7441 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     7501 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     7561 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     7621 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     7681 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     7741 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc
     7801 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7861 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7921 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7981 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     8041 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     8101 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     8161 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     8221 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     8281 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     8341 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     8401 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     8461 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     8521 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     8581 ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga
     8641 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     8701 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     8761 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     8821 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     8881 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     8941 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     9001 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     9061 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     9121 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     9181 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     9241 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     9301 agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg
     9361 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9421 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9481 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9541 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9601 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9661 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9721 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9781 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9841 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9901 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9961 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10021 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10081 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10141 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10201 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10261 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10321 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10381 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10441 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10501 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10561 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10621 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10681 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10741 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10801 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10861 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10921 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10981 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    11041 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    11101 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    11161 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    11221 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    11281 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    11341 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11401 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11461 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11521 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
    11581 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    11641 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    11701 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    11761 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    11821 aatatgtact acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca
    11881 aacttagtag t
//
