LOCUS       V020003                11487 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020003
VERSION     V020003
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2827..6216
                     /label="LATS1(NM_004690)"
                     /note="LATS1(NM_004690)"
                     /gene="LATS1"
     CDS             6217..6243
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        6275..6774
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6795..7394
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7408..7996
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8203..8836
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8965..8981)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8989..9005
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9013..9043)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9058..9079
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9367..9955)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10126..10986)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10987..11091)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11139..11273
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatga agaggagtga aaagccagaa ggatatagac aaatgaggcc taagaccttt
     2881 cctgccagta actatactgt cagtagccgg caaatgttac aagaaattcg ggaatccctt
     2941 aggaatttat ctaaaccatc tgatgctgct aaggctgagc ataacatgag taaaatgtca
     3001 accgaagatc ctcgacaagt cagaaatcca cccaaatttg ggacgcatca taaagccttg
     3061 caggaaattc gaaactctct gcttccattt gcaaatgaaa caaattcttc tcggagtact
     3121 tcagaagtta atccacaaat gcttcaagac ttgcaagctg ctggatttga tgaggatatg
     3181 gttatacaag ctcttcagcg aactaacaac agaagtatag aagcagcaat tgaattcatt
     3241 agtaaaatga gttaccaaga tcctcgacga gagcagatgg ctgcagcagc tgccagacct
     3301 attaatgcca gcatgaaacc agggaatgtg cagcaatcag ttaaccgcaa acagagctgg
     3361 aaaggttcta aagaatcctt agttcctcag aggcatggcc cgccactagg agaaagtgtg
     3421 gcctatcatt ctgagagtcc caactcacag acagatgtag gaagaccttt gtctggatct
     3481 ggtatatcag catttgttca agctcaccct agcaacggac agagagtgaa ccccccacca
     3541 ccacctcaag taaggagtgt tactcctcca ccacctccaa gaggccagac tccccctcca
     3601 agaggtacaa ctccacctcc cccttcatgg gaaccaaact ctcaaacaaa gcgctattct
     3661 ggaaacatgg aatacgtaat ctcccgaatc tctcctgtcc cacctggggc atggcaagag
     3721 ggctatcctc caccacctct caacacttcc cccatgaatc ctcctaatca aggacagaga
     3781 ggcattagtt ctgttcctgt tggcagacaa ccaatcatca tgcagagttc tagcaaattt
     3841 aactttccat cagggagacc tggaatgcag aatggtactg gacaaactga tttcatgata
     3901 caccaaaatg ttgtccctgc tggcactgtg aatcggcagc caccacctcc atatcctctg
     3961 acagcagcta atggacaaag cccttctgct ttacaaacag ggggatctgc tgctccttcg
     4021 tcatatacaa atggaagtat tcctcagtct atgatggtgc caaacagaaa tagtcataac
     4081 atggaactat ataacattag tgtacctgga ctgcaaacaa attggcctca gtcatcttct
     4141 gctccagccc agtcatcccc gagcagtggg catgaaatcc ctacatggca acctaacata
     4201 ccagtgaggt caaattcttt taataaccca ttaggaaata gagcaagtca ctctgctaat
     4261 tctcagcctt ctgctacaac agtcactgca attacaccag ctcctattca acagcctgtg
     4321 aaaagtatgc gtgtattaaa accagagcta cagactgctt tagcacctac acacccttct
     4381 tggataccac agccaattca aactgttcaa cccagtcctt ttcctgaggg aaccgcttca
     4441 aatgtgactg tgatgccacc tgttgctgaa gctccaaact atcaaggacc accaccaccc
     4501 tacccaaaac atctgctgca ccaaaaccca tctgttcctc catacgagtc aatcagtaag
     4561 cctagcaaag aggatcagcc aagcttgccc aaggaagatg agagtgaaaa gagttatgaa
     4621 aatgttgata gtggggataa agaaaagaaa cagattacaa cttcacctat tactgttagg
     4681 aaaaacaaga aagatgaaga gcgaagggaa tctcgtattc aaagttattc tcctcaagca
     4741 tttaaattct ttatggagca acatgtagaa aatgtactca aatctcatca gcagcgtcta
     4801 catcgtaaaa aacaattaga gaatgaaatg atgcgggttg gattatctca agatgcccag
     4861 gatcaaatga gaaagatgct ttgccaaaaa gaatctaatt acatccgtct taaaagggct
     4921 aaaatggaca agtctatgtt tgtgaagata aagacactag gaataggagc atttggtgaa
     4981 gtctgtctag caagaaaagt agatactaag gctttgtatg caacaaaaac tcttcgaaag
     5041 aaagatgttc ttcttcgaaa tcaagtcgct catgttaagg ctgagagaga tatcctggct
     5101 gaagctgaca atgaatgggt agttcgtcta tattattcat tccaagataa ggacaattta
     5161 tactttgtaa tggactacat tcctgggggt gatatgatga gcctattaat tagaatgggc
     5221 atctttccag aaagtctggc acgattctac atagcagaac ttacctgtgc agttgaaagt
     5281 gttcataaaa tgggttttat tcatagagat attaaacctg ataatatttt gattgatcgt
     5341 gatggtcata ttaaattgac tgactttggc ctctgcactg gcttcagatg gacacacgat
     5401 tctaagtact atcagagtgg tgaccatcca cggcaagata gcatggattt cagtaatgaa
     5461 tggggggatc cctcaagctg tcgatgtgga gacagactga agccattaga gcggagagct
     5521 gcacgccagc accagcgatg tctagcacat tctttggttg ggactcccaa ttatattgca
     5581 cctgaagtgt tgctacgaac aggatacaca cagttgtgtg attggtggag tgttggtgtt
     5641 attctttttg aaatgttggt gggacaacct cctttcttgg cacaaacacc attagaaaca
     5701 caaatgaagg ttatcaactg gcaaacatct cttcacattc caccacaagc taaactcagt
     5761 cctgaagctt ctgatcttat tattaaactt tgccgaggac ccgaagatcg cttaggcaag
     5821 aatggtgctg atgaaataaa agctcatcca ttttttaaaa caattgactt ctccagtgac
     5881 ctgagacagc agtctgcttc atacattcct aaaatcacac acccaacaga tacatcaaat
     5941 tttgatcctg ttgatcctga taaattatgg agtgatgata acgaggaaga aaatgtaaat
     6001 gacactctca atggatggta taaaaatgga aagcatcctg aacatgcatt ctatgaattt
     6061 accttccgaa ggttttttga tgacaatggc tacccatata attatccgaa gcctattgaa
     6121 tatgaataca ttaattcaca aggctcagag cagcagtcgg atgaagatga tcaaaacaca
     6181 ggctcagaga ttaaaaatcg cgatctagta tatgtttacc catacgacgt accagattac
     6241 gcttaaggat cccgcgactc tagataattc taccgggtag gggaggcgct tttcccaagg
     6301 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     6361 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     6421 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     6481 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     6541 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     6601 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     6661 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     6721 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     6781 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     6841 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     6901 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     6961 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     7021 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     7081 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     7141 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     7201 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     7261 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     7321 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     7381 agcccggtgc ctgaccgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
     7441 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7501 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7561 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7621 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7681 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7741 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7801 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7861 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7921 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7981 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
     8041 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     8101 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     8161 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact
     8221 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     8281 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     8341 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     8401 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     8461 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     8521 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     8581 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     8641 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8701 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8761 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8821 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     8881 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgttttac cgtcgacctc
     8941 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     9001 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     9061 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9121 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9181 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9241 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9301 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9361 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9421 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9481 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9541 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9601 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9661 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9721 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9781 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9841 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9901 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9961 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    10021 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    10081 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    10141 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10201 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10261 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    10321 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10381 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10441 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10501 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10561 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10621 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10681 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10741 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10801 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10861 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10921 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10981 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    11041 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    11101 ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata
    11161 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    11221 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    11281 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    11341 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    11401 gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa attgtatttg
    11461 ttaaatatgt actacaaact tagtagt
//
