LOCUS V029670 10066 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029670 VERSION V029670 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..4093 /codon_start=1 /gene="" /note="" /product="human lysosome-associated membrane glycoprotein 1" /protein_id="" /transl_table=1 /translation="MAAPGSARRPLLLLLLLLLLGLMHCASA,AMFMVKNGNGTACIMA NFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLN FTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVH MNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDK YNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHS EGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCN AEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLI VLIAYLVGRKRSHAGYQTI" /label="LAMP1" CDS 4118..4837 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" promoter 4854..5353 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5374..5973 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5987..6575 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6782..7415 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7544..7560) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7568..7584 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7592..7622) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7637..7658 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7946..8534) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8705..9565) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9566..9670) /gene="bla" /label="AmpR promoter" polyA_signal 9718..9852 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggcggc ccccggcagc gcccggcgac ccctgctgct 2881 gctactgctg ttgctgctgc tcggcctcat gcattgtgcg tcagcagcaa tgtttatggt 2941 gaaaaatggc aacgggaccg cgtgcataat ggccaacttc tctgctgcct tctcagtgaa 3001 ctacgacacc aagagtggcc ctaagaacat gacctttgac ctgccatcag atgccacagt 3061 ggtgctcaac cgcagctcct gtggaaaaga gaacacttct gaccccagtc tcgtgattgc 3121 ttttggaaga ggacatacac tcactctcaa tttcacgaga aatgcaacac gttacagcgt 3181 ccagctcatg agttttgttt ataacttgtc agacacacac cttttcccca atgcgagctc 3241 caaagaaatc aagactgtgg aatctataac tgacatcagg gcagatatag ataaaaaata 3301 cagatgtgtt agtggcaccc aggtccacat gaacaacgtg accgtaacgc tccatgatgc 3361 caccatccag gcgtaccttt ccaacagcag cttcagccgg ggagagacac gctgtgaaca 3421 agacaggcct tccccaacca cagcgccccc tgcgccaccc agcccctcgc cctcacccgt 3481 gcccaagagc ccctctgtgg acaagtacaa cgtgagcggc accaacggga cctgcctgct 3541 ggccagcatg gggctgcagc tgaacctcac ctatgagagg aaggacaaca cgacggtgac 3601 aaggcttctc aacatcaacc ccaacaagac ctcggccagc gggagctgcg gcgcccacct 3661 ggtgactctg gagctgcaca gcgagggcac caccgtcctg ctcttccagt tcgggatgaa 3721 tgcaagttct agccggtttt tcctacaagg aatccagttg aatacaattc ttcctgacgc 3781 cagagaccct gcctttaaag ctgccaacgg ctccctgcga gcgctgcagg ccacagtcgg 3841 caattcctac aagtgcaacg cggaggagca cgtccgtgtc acgaaggcgt tttcagtcaa 3901 tatattcaaa gtgtgggtcc aggctttcaa ggtggaaggt ggccagtttg gctctgtgga 3961 ggagtgtctg ctggacgaga acagcatgct gatccccatc gctgtgggtg gtgccctggc 4021 ggggctggtc ctcatcgtcc tcatcgccta cctcgtcggc aggaagagga gtcacgcagg 4081 ctaccagact atcggaggtg gtggaggatc cgccaccatg gtgagcaagg gcgaggagct 4141 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 4201 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 4261 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg 4321 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 4381 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 4441 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 4501 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 4561 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat 4621 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 4681 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 4741 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 4801 cgggatcact ctcggcatgg acgagctgta caagtagtct agataattct accgggtagg 4861 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg 4921 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc 4981 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt 5041 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac 5101 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag 5161 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc 5221 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg 5281 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc 5341 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc 5401 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 5461 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 5521 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 5581 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 5641 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 5701 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 5761 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 5821 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 5881 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 5941 acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga 6001 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 6061 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 6121 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 6181 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 6241 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 6301 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 6361 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 6421 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 6481 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 6541 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac 6601 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 6661 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta 6721 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga 6781 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac 6841 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 6901 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 6961 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 7021 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 7081 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 7141 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 7201 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 7261 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 7321 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 7381 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 7441 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta 7501 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 7561 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 7621 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 7681 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 7741 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 7801 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 7861 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 7921 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 7981 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 8041 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 8101 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 8161 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 8221 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 8281 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 8341 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 8401 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 8461 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 8521 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 8581 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 8641 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 8701 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 8761 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 8821 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 8881 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 8941 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 9001 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 9061 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 9121 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 9181 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 9241 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 9301 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 9361 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 9421 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 9481 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 9541 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 9601 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 9661 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac 9721 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 9781 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 9841 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca 9901 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct 9961 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt 10021 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt //