LOCUS       V039851                10404 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039851
VERSION     V039851
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2827..3543
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3559..3618
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from equine rhinitis A virus
                     polyprotein"
                     /transl_table=1
                     /translation="QCTNYALLKLAGDVESNPGP"
                     /label="E2A"
     CDS             3631..5160
                     /label="INSM1(NM_002196)"
                     /note="INSM1(NM_002196)"
                     /gene="INSM1"
     promoter        5192..5691
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5712..6311
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6325..6913
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7120..7753
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7882..7898)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7906..7922
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7930..7960)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7975..7996
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8284..8872)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9043..9903)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9904..10008)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10056..10190
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2881 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     2941 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     3001 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     3061 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3121 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3181 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3241 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     3301 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     3361 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     3421 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     3481 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     3541 aaggaatttg gcagcggcca gtgcaccaac tatgccctgc tgaaactggc tggcgatgtg
     3601 gaaagcaacc ccggacccga attcgccacc atgccccgcg gcttcctggt gaagcgcagc
     3661 aagaagtcca cgcccgtttc ctaccgggtc cgcggcggcg aggacggcga ccgcgcactg
     3721 ctgctctcgc ccagctgcgg gggcgcccgc gccgagcccc cggcgccgag cccggtcccc
     3781 gggccgctgc cgccgccgcc gcccgcggag cgcgcccatg cagcgctcgc cgccgcgctt
     3841 gcctgcgcgc ctgggccgca gccacccccg cagggcccgc gggccgcgca cttcggcaac
     3901 cccgaggctg cgcaccccgc gccgctctac agtcccacgc ggcccgtgag ccgcgagcac
     3961 gagaagcaca agtacttcga acgcagcttc aacctgggct cgccggtctc ggccgagtcc
     4021 ttccccacgc ccgccgcgct gctcggaggg ggcggcggcg gcggcgcgag cggagctggc
     4081 ggaggcggca cctgcggcgg cgacccgctg ctcttcgcgc ccgccgagct caagatgggc
     4141 acggcgttct cggctggcgc cgaggcggcc cgcggcccgg gccccggccc cccactgccc
     4201 cctgccgccg ccctgcggcc cccgggaaag cggcccccgc cccctaccgc cgcggagccg
     4261 cccgccaagg cagtcaaggc cccgggcgcc aagaagccca aggccatccg caagctgcac
     4321 ttcgaggacg aggtgaccac gtcgcccgtg ctggggctca agatcaagga gggcccggtg
     4381 gaggcgccgc ggggccgcgc ggggggcgcg gcgcggccgc tgggcgagtt catctgccag
     4441 ctgtgcaagg aggagtacgc cgacccgttc gcgctggcgc agcacaaatg ctcgcgcatc
     4501 gtgcgtgtgg agtaccgctg tcccgagtgc gccaaggtct tcagctgccc ggccaacctg
     4561 gcctcgcacc gccgctggca caaaccgcgg cccgcgcccg ccgccgcccg cgcgccggag
     4621 ccagaagcag cagccagggc tgaggcgcgg gaggcacccg gcggcggcag cgaccgggac
     4681 acgccgagcc ccggcggcgt gtccgagtcg ggctccgagg acgggctcta cgagtgccat
     4741 cactgcgcca agaagttccg ccgccaggcc tacctacgca agcacctgct ggcgcaccac
     4801 caggcgctgc aggccaaggg cgcgccgcta gcgcccccgg ccgaggacct actggccttg
     4861 taccccgggc ccgacgagaa ggcgccccag gaggcggccg gcgacggcga gggggccggc
     4921 gtgctgggcc tgagtgcgtc cgccgagtgc cacctgtgcc cagtgtgcgg agagtcgttc
     4981 gccagcaagg gcgctcagga gcgccacctg cgcctgctgc acgccgccca ggtgttcccc
     5041 tgcaagtact gcccggccac cttctacagc tcgcccggcc ttacgcggca catcaacaag
     5101 tgccacccat ccgaaaacag acaggtgatc ctcctgcagg tgcccgtgcg cccggcctgc
     5161 tagggatccc gcgactctag ataattctac cgggtagggg aggcgctttt cccaaggcag
     5221 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     5281 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     5341 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     5401 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     5461 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     5521 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     5581 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     5641 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
     5701 ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     5761 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     5821 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     5881 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     5941 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     6001 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     6061 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     6121 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     6181 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6241 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6301 ccggtgcctg accgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg
     6361 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6421 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6481 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6541 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6601 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6661 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     6721 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6781 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6841 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6901 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca
     6961 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7021 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7081 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
     7141 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7201 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     7261 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     7321 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     7381 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     7441 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     7501 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     7561 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7621 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7681 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7741 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7801 aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag
     7861 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7921 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7981 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8041 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8101 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8161 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8221 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8281 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8341 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8401 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8461 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8521 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8581 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8641 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8701 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8761 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8821 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8881 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8941 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9001 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9061 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9121 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9181 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9241 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9301 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9361 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9421 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9481 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9541 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9601 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9661 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9721 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9781 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9841 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9901 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9961 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10021 aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg
    10081 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10141 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    10201 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    10261 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    10321 attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt gtatttgtta
    10381 aatatgtact acaaacttag tagt
//
