LOCUS       V025917                12928 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025917
VERSION     V025917
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..6943
                     /label="IGF1R(NM_000875)"
                     /note="IGF1R(NM_000875)"
                     /gene="IGF1R"
     CDS             6980..7699
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        7716..8215
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             8236..8835
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8849..9437
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9644..10277
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(10406..10422)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10430..10446
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10454..10484)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10499..10520
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10808..11396)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11567..12427)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12428..12532)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    12580..12714
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgaagtc tggctccgga ggagggtccc cgacctcgct
     2881 gtgggggctc ctgtttctct ccgccgcgct ctcgctctgg ccgacgagtg gagaaatctg
     2941 cgggccaggc atcgacatcc gcaacgacta tcagcagctg aagcgcctgg agaactgcac
     3001 ggtgatcgag ggctacctcc acatcctgct catctccaag gccgaggact accgcagcta
     3061 ccgcttcccc aagctcacgg tcattaccga gtacttgctg ctgttccgag tggctggcct
     3121 cgagagcctc ggagacctct tccccaacct cacggtcatc cgcggctgga aactcttcta
     3181 caactacgcc ctggtcatct tcgagatgac caatctcaag gatattgggc tttacaacct
     3241 gaggaacatt actcgggggg ccatcaggat tgagaaaaat gctgacctct gttacctctc
     3301 cactgtggac tggtccctga tcctggatgc ggtgtccaat aactacattg tggggaataa
     3361 gcccccaaag gaatgtgggg acctgtgtcc agggaccatg gaggagaagc cgatgtgtga
     3421 gaagaccacc atcaacaatg agtacaacta ccgctgctgg accacaaacc gctgccagaa
     3481 aatgtgccca agcacgtgtg ggaagcgggc gtgcaccgag aacaatgagt gctgccaccc
     3541 cgagtgcctg ggcagctgca gcgcgcctga caacgacacg gcctgtgtag cttgccgcca
     3601 ctactactat gccggtgtct gtgtgcctgc ctgcccgccc aacacctaca ggtttgaggg
     3661 ctggcgctgt gtggaccgtg acttctgcgc caacatcctc agcgccgaga gcagcgactc
     3721 cgaggggttt gtgatccacg acggcgagtg catgcaggag tgcccctcgg gcttcatccg
     3781 caacggcagc cagagcatgt actgcatccc ttgtgaaggt ccttgcccga aggtctgtga
     3841 ggaagaaaag aaaacaaaga ccattgattc tgttacttct gctcagatgc tccaaggatg
     3901 caccatcttc aagggcaatt tgctcattaa catccgacgg gggaataaca ttgcttcaga
     3961 gctggagaac ttcatggggc tcatcgaggt ggtgacgggc tacgtgaaga tccgccattc
     4021 tcatgccttg gtctccttgt ccttcctaaa aaaccttcgc ctcatcctag gagaggagca
     4081 gctagaaggg aattactcct tctacgtcct cgacaaccag aacttgcagc aactgtggga
     4141 ctgggaccac cgcaacctga ccatcaaagc agggaaaatg tactttgctt tcaatcccaa
     4201 attatgtgtt tccgaaattt accgcatgga ggaagtgacg gggactaaag ggcgccaaag
     4261 caaaggggac ataaacacca ggaacaacgg ggagagagcc tcctgtgaaa gtgacgtcct
     4321 gcatttcacc tccaccacca cgtcgaagaa tcgcatcatc ataacctggc accggtaccg
     4381 gccccctgac tacagggatc tcatcagctt caccgtttac tacaaggaag caccctttaa
     4441 gaatgtcaca gagtatgatg ggcaggatgc ctgcggctcc aacagctgga acatggtgga
     4501 cgtggacctc ccgcccaaca aggacgtgga gcccggcatc ttactacatg ggctgaagcc
     4561 ctggactcag tacgccgttt acgtcaaggc tgtgaccctc accatggtgg agaacgacca
     4621 tatccgtggg gccaagagtg agatcttgta cattcgcacc aatgcttcag ttccttccat
     4681 tcccttggac gttctttcag catcgaactc ctcttctcag ttaatcgtga agtggaaccc
     4741 tccctctctg cccaacggca acctgagtta ctacattgtg cgctggcagc ggcagcctca
     4801 ggacggctac ctttaccggc acaattactg ctccaaagac aaaatcccca tcaggaagta
     4861 tgccgacggc accatcgaca ttgaggaggt cacagagaac cccaagactg aggtgtgtgg
     4921 tggggagaaa gggccttgct gcgcctgccc caaaactgaa gccgagaagc aggccgagaa
     4981 ggaggaggct gaataccgca aagtctttga gaatttcctg cacaactcca tcttcgtgcc
     5041 cagacctgaa aggaagcgga gagatgtcat gcaagtggcc aacaccacca tgtccagccg
     5101 aagcaggaac accacggccg cagacaccta caacatcacc gacccggaag agctggagac
     5161 agagtaccct ttctttgaga gcagagtgga taacaaggag agaactgtca tttctaacct
     5221 tcggcctttc acattgtacc gcatcgatat ccacagctgc aaccacgagg ctgagaagct
     5281 gggctgcagc gcctccaact tcgtctttgc aaggactatg cccgcagaag gagcagatga
     5341 cattcctggg ccagtgacct gggagccaag gcctgaaaac tccatctttt taaagtggcc
     5401 ggaacctgag aatcccaatg gattgattct aatgtatgaa ataaaatacg gatcacaagt
     5461 tgaggatcag cgagaatgtg tgtccagaca ggaatacagg aagtatggag gggccaagct
     5521 aaaccggcta aacccgggga actacacagc ccggattcag gccacatctc tctctgggaa
     5581 tgggtcgtgg acagatcctg tgttcttcta tgtccaggcc aaaacaggat atgaaaactt
     5641 catccatctg atcatcgctc tgcccgtcgc tgtcctgttg atcgtgggag ggttggtgat
     5701 tatgctgtac gtcttccata gaaagagaaa taacagcagg ctggggaatg gagtgctgta
     5761 tgcctctgtg aacccggagt acttcagcgc tgctgatgtg tacgttcctg atgagtggga
     5821 ggtggctcgg gagaagatca ccatgagccg ggaacttggg caggggtcgt ttgggatggt
     5881 ctatgaagga gttgccaagg gtgtggtgaa agatgaacct gaaaccagag tggccattaa
     5941 aacagtgaac gaggccgcaa gcatgcgtga gaggattgag tttctcaacg aagcttctgt
     6001 gatgaaggag ttcaattgtc accatgtggt gcgattgctg ggtgtggtgt cccaaggcca
     6061 gccaacactg gtcatcatgg aactgatgac acggggcgat ctcaaaagtt atctccggtc
     6121 tctgaggcca gaaatggaga ataatccagt cctagcacct ccaagcctga gcaagatgat
     6181 tcagatggcc ggagagattg cagacggcat ggcatacctc aacgccaata agttcgtcca
     6241 cagagacctt gctgcccgga attgcatggt agccgaagat ttcacagtca aaatcggaga
     6301 ttttggtatg acgcgagata tctatgagac agactattac cggaaaggag ggaaagggct
     6361 gctgcccgtg cgctggatgt ctcctgagtc cctcaaggat ggagtcttca ccacttactc
     6421 ggacgtctgg tccttcgggg tcgtcctctg ggagatcgcc acactggccg agcagcccta
     6481 ccagggcttg tccaacgagc aagtccttcg cttcgtcatg gagggcggcc ttctggacaa
     6541 gccagacaac tgtcctgaca tgctgtttga actgatgcgc atgtgctggc agtataaccc
     6601 caagatgagg ccttccttcc tggagatcat cagcagcatc aaagaggaga tggagcctgg
     6661 cttccgggag gtctccttct actacagcga ggagaacaag ctgcccgagc cggaggagct
     6721 ggacctggag ccagagaaca tggagagcgt ccccctggac ccctcggcct cctcgtcctc
     6781 cctgccactg cccgacagac actcaggaca caaggccgag aacggccccg gccctggggt
     6841 gctggtcctc cgcgccagct tcgacgagag acagccttac gcccacatga acgggggccg
     6901 caagaacgag cgggccttgc cgctgcccca gtcttcgacc tgcggcggcg gagggagtgg
     6961 aggaggggga agcggatcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     7021 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     7081 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     7141 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     7201 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     7261 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     7321 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     7381 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     7441 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     7501 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     7561 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     7621 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     7681 ggacgagctg tacaagtagt ctagataatt ctaccgggta ggggaggcgc ttttcccaag
     7741 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     7801 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     7861 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     7921 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     7981 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     8041 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     8101 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     8161 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     8221 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     8281 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     8341 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     8401 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     8461 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     8521 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     8581 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     8641 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     8701 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     8761 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     8821 aagcccggtg cctgaccgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag
     8881 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     8941 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     9001 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     9061 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     9121 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     9181 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     9241 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     9301 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     9361 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     9421 ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca
     9481 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     9541 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     9601 gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac
     9661 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     9721 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
     9781 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
     9841 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
     9901 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
     9961 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
    10021 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
    10081 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    10141 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    10201 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    10261 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
    10321 aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttta ccgtcgacct
    10381 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    10441 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    10501 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    10561 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    10621 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    10681 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    10741 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    10801 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    10861 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    10921 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    10981 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    11041 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    11101 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    11161 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    11221 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    11281 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    11341 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    11401 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    11461 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    11521 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    11581 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    11641 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    11701 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    11761 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    11821 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    11881 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    11941 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    12001 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    12061 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    12121 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    12181 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    12241 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    12301 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    12361 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    12421 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    12481 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    12541 cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat
    12601 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    12661 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    12721 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    12781 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    12841 agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt
    12901 gttaaatatg tactacaaac ttagtagt
//
