LOCUS       V018349                11547 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018349
VERSION     V018349
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2827..6273
                     /label="Hdac6(NM_010413)"
                     /note="Hdac6(NM_010413)"
                     /gene="Hdac6"
     CDS             6280..6303
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     promoter        6335..6834
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6855..7454
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7468..8056
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8263..8896
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9025..9041)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9049..9065
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9073..9103)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9118..9139
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9427..10015)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10186..11046)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11047..11151)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11199..11333
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatga cctccaccgg ccaagattct tctactagac agcgaaagag taggcacaat
     2881 ccccagtcac cccttcagga atccagcgcc accttgaagc gtggtggaaa gaagtgtgct
     2941 gtaccccact ccagccccaa tctagcggag gtaaagaaga aaggcaaaat gaagaagctg
     3001 agccaaccag ctgaagagga cctagttgtg gggcttcaag ggctggatct gaaccctgag
     3061 acaagagtgc cagttggtac tggattggtg tttgatgaac aactaaatga cttccattgc
     3121 ctttgggatg acagcttccc tgaaagccct gagcggctcc atgccatcag agagcaactg
     3181 atcctggagg gcctcctggg ccgctgtgtg tcctttcagg cccggttcgc tgagaaggag
     3241 gagctgatgt tggttcacag cctggaatac attgatctga tggagacaac ccagtacatg
     3301 aatgaagggg agcttcgagt actggcagaa acctatgatt cagtgtatct gcatccgaac
     3361 tcatattcct gtgcctgcct ggctacaggc tctgtcctcc ggctggtaga tgcactcatg
     3421 ggggctgaga ttcggaatgg catggccgtc atcaggcctc ctggacacca tgctcagcac
     3481 aatcttatgg atgggtattg catgttcaac catctggctg tggctgcccg ctatgcgcaa
     3541 aagaagcacc gcattcagag ggttctcatc gtggactggg atgtgcacca tggtcaagga
     3601 acacagttca tcttcgacca ggaccccagt gtcctttatt tctccatcca ccgatatgaa
     3661 catggtcgct tctggcccca ccttaaggct tctaactggt ccactatagg ttttggccaa
     3721 ggccaaggat ataccatcaa tgtaccttgg aaccagacgg gcatgcggga cgctgactac
     3781 attgctgctt tcctgcacat cctgctgcca gttgcctcgg agtttcagcc tcaactggtc
     3841 ttggtggccg ctggatttga tgccctccac ggagacccca agggagagat ggctgccacg
     3901 ccagcaggat ttgcccacct aacccatttg ctcatgggtt tggcaggagg caagttgatt
     3961 ctgtccctgg agggtggcta taacctccgt gccctggcta agggagtcag tgcttcactc
     4021 cacacccttc ttggagaccc ttgccccatg ctggagtcct gtgttgtacc ttgtgcaagc
     4081 gcccagactt ccatctactg cactctagaa gcccttgaac ccttctggga ggtcctggag
     4141 agatcagttg agacccagga ggaagatgaa gtggaagaag ccgtgctaga agaggaggag
     4201 gaggaaggtg gctgggaggc cactgcactg cccatggata catggccact gctccagaac
     4261 cgcactgggc tggtctatga tgagaagatg atgagtcact gcaacctctg ggacaatcat
     4321 caccctgaga cacctcagcg catcttacgc atcatgtgtc acctggagga ggtgggcctt
     4381 gcggctcgct gtctcatcct acctgctcgg cctgccctgg actctgagct ccttacctgc
     4441 cacagtgctg agtacgtgga gcatctccgc accacagaaa agatgaaaac ccgggatctg
     4501 caccgtgaag gtgccaactt tgactccatc tacatctgcc ccagcacctt tgcctgcgca
     4561 aagcttgcca caggcgctgc ctgccgcctg gtggaagctg tgctctcggg agaggttcta
     4621 aatggcattg cagtagtgcg ccctccagga caccacgcgg agccaaatgc tgcctgtggt
     4681 ttctgctttt tcaactcagt agctgtagct gctcgccatg cccagatcat tgctggacgt
     4741 gccctgcgga tcctaatcgt ggattgggat gttcatcatg gtaatggaac tcagcacata
     4801 tttgaggatg accctagtgt attatacgtg tccctgcacc ggtatgaccg tggcactttc
     4861 ttccccatgg gggatgaggg tgccagtagc caagtaggcc gagatgcagg tataggcttc
     4921 actgtcaatg tgccctggaa tgggccccgc atgggtgatg ctgattacct ggctgcatgg
     4981 catcgtctgg tacttcccat cgcctatgag tttaacccag aactggtgct gatctcagct
     5041 ggctttgatg ctgcacaagg ggatccgctg gggggctgcc aagtaacacc ggagggttat
     5101 gcccacctca cccacctact gatgggcctt gctggtggcc gtattattct tattctagag
     5161 ggtggataca atttggcatc tatctctgag tctatggctg cctgcaccca ttccctcctt
     5221 ggagacccac caccccagct tactttgctg cgaccgccac agtcaggagc cctggtttca
     5281 atcagtgagg tcatccaagt ccatcgcaaa tactggcgca gtttgcggtt gatgaaaatg
     5341 gaagacaagg aagaatgctc tagttctagg cttgtcatca agaagttgcc cccaacagcc
     5401 agtcctgtat cagctaagga aatgaccaca ccgaaaggaa aggttcctga agaaagcgtg
     5461 aggaagacca tagcagcact acctgggaaa gagtctactc taggccaggc taaatcaaag
     5521 atggctaagg ctgtgcttgc tcagggccag tcctcagaac aagctgctaa gggaactaca
     5581 ctggatctgg ctacctcaaa ggagactgtg ggaggagcca cgacggacct gtgggcctca
     5641 gcagctgctc ctgaaaactt ccctaaccag accacctctg tggaggcttt gggagaaact
     5701 gagccaacgc ctccagcctc gcatacaaac aagcagacca caggggcttc acctctgcag
     5761 ggagtcacgg ctcagcagtc cctacagctt ggggttctca gcactttgga gctaagcaga
     5821 gaagcagagg aagcccatga ttctgaggag ggcctgctag gggaagccgc tggaggtcag
     5881 gacatgaaca gcttgatgct gacacaagga tttggggact ttaataccca ggatgtattt
     5941 tatgctgtga ccccactatc ctggtgtccc catttgatgg cagtatgccc cattcctgca
     6001 gcaggcctag atgtgtccca accttgtaag acctgtggaa cagtccagga gaactgggtg
     6061 tgtctgactt gctatcaggt gtactgcagt cgctatgtca atgcccatat ggtctgccac
     6121 catgaagcct ctgaacaccc gctggtcctc agctgtgttg acctgtctac ctggtgttat
     6181 gtctgtcagg cttatgtcca ccacgaggat ctccaagatg tgaagaacgc tgcccaccag
     6241 aacaagtttg gggaggacat gccccactca cacgaattcg attacaagga tgacgacgat
     6301 aagtgaggat cccgcgactc tagataattc taccgggtag gggaggcgct tttcccaagg
     6361 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     6421 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     6481 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     6541 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     6601 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     6661 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     6721 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     6781 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     6841 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     6901 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     6961 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     7021 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     7081 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     7141 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     7201 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     7261 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     7321 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     7381 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     7441 agcccggtgc ctgaccgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
     7501 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7561 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7621 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7681 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7741 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7801 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7861 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7921 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7981 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8041 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
     8101 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     8161 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     8221 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact
     8281 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     8341 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     8401 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     8461 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     8521 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     8581 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     8641 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     8701 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8761 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8821 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8881 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     8941 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgttttac cgtcgacctc
     9001 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     9061 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     9121 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9181 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9241 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9301 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9361 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9421 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9481 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9541 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9601 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9661 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9721 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9781 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9841 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9901 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9961 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    10021 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    10081 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    10141 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    10201 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10261 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10321 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    10381 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10441 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10501 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10561 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10621 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10681 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10741 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10801 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10861 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10921 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10981 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    11041 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    11101 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    11161 ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata
    11221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    11281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    11341 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    11401 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    11461 gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa attgtatttg
    11521 ttaaatatgt actacaaact tagtagt
//
