LOCUS       V018913                10798 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018913
VERSION     V018913
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..3559
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3629..5566
                     /label="HSPA8(NM_006597)"
                     /note="HSPA8(NM_006597)"
                     /gene="HSPA8"
     promoter        5586..6085
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6106..6705
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6719..7307
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7514..8147
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8276..8292)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8300..8316
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8324..8354)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8369..8390
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8678..9266)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9437..10297)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10298..10402)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10450..10584
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     2881 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2941 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     3001 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     3061 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     3121 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     3181 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     3241 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     3301 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     3361 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     3421 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     3481 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     3541 catggacgag ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg
     3601 gggaagcgga ggcggaggga gtggatccat gtccaaggga cctgcagttg gtattgatct
     3661 tggcaccacc tactcttgtg tgggtgtttt ccagcacgga aaagtcgaga taattgccaa
     3721 tgatcaggga aaccgaacca ctccaagcta tgtcgccttt acggacactg aacggttgat
     3781 cggtgatgcc gcaaagaatc aagttgcaat gaaccccacc aacacagttt ttgatgccaa
     3841 acgtctgatt ggacgcagat ttgatgatgc tgttgtccag tctgatatga aacattggcc
     3901 ctttatggtg gtgaatgatg ctggcaggcc caaggtccaa gtagaataca agggagagac
     3961 caaaagcttc tatccagagg aggtgtcttc tatggttctg acaaagatga aggaaattgc
     4021 agaagcctac cttgggaaga ctgttaccaa tgctgtggtc acagtgccag cttactttaa
     4081 tgactctcag cgtcaggcta ccaaagatgc tggaactatt gctggtctca atgtacttag
     4141 aattattaat gagccaactg ctgctgctat tgcttacggc ttagacaaaa aggttggagc
     4201 agaaagaaac gtgctcatct ttgacctggg aggtggcact tttgatgtgt caatcctcac
     4261 tattgaggat ggaatctttg aggtcaagtc tacagctgga gacacccact tgggtggaga
     4321 agattttgac aaccgaatgg tcaaccattt tattgctgag tttaagcgca agcataagaa
     4381 ggacatcagt gagaacaaga gagctgtaag acgcctccgt actgcttgtg aacgtgctaa
     4441 gcgtaccctc tcttccagca cccaggccag tattgagatc gattctctct atgaaggaat
     4501 cgacttctat acctccatta cccgtgcccg atttgaagaa ctgaatgctg acctgttccg
     4561 tggcaccctg gacccagtag agaaagccct tcgagatgcc aaactagaca agtcacagat
     4621 tcatgatatt gtcctggttg gtggttctac tcgtatcccc aagattcaga agcttctcca
     4681 agacttcttc aatggaaaag aactgaataa gagcatcaac cctgatgaag ctgttgctta
     4741 tggtgcagct gtccaggcag ccatcttgtc tggagacaag tctgagaatg ttcaagattt
     4801 gctgctcttg gatgtcactc ctctttccct tggtattgaa actgctggtg gagtcatgac
     4861 tgtcctcatc aagcgtaata ccaccattcc taccaagcag acacagacct tcactaccta
     4921 ttctgacaac cagcctggtg tgcttattca ggtttatgaa ggcgagcgtg ccatgacaaa
     4981 ggataacaac ctgcttggca agtttgaact cacaggcata cctcctgcac cccgaggtgt
     5041 tcctcagatt gaagtcactt ttgacattga tgccaatggt atactcaatg tctctgctgt
     5101 ggacaagagt acgggaaaag agaacaagat tactatcact aatgacaagg gccgtttgag
     5161 caaggaagac attgaacgta tggtccagga agctgagaag tacaaagctg aagatgagaa
     5221 gcagagggac aaggtgtcat ccaagaattc acttgagtcc tatgccttca acatgaaagc
     5281 aactgttgaa gatgagaaac ttcaaggcaa gattaacgat gaggacaaac agaagattct
     5341 ggacaagtgt aatgaaatta tcaactggct tgataagaat cagactgctg agaaggaaga
     5401 atttgaacat caacagaaag agctggagaa agtttgcaac cccatcatca ccaagctgta
     5461 ccagagtgca ggaggcatgc caggaggaat gcctggggga tttcctggtg gtggagctcc
     5521 tccctctggt ggtgcttcct cagggcccac cattgaagag gttgattaat ctagataatt
     5581 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     5641 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     5701 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     5761 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     5821 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     5881 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
     5941 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
     6001 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
     6061 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag
     6121 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6181 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6241 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6301 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6361 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6421 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6481 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6541 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6601 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6661 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaccgcg tctggaacaa
     6721 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6781 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6841 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6901 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6961 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7021 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7081 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     7141 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7201 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7261 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc
     7321 tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     7381 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     7441 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7501 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     7561 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     7621 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     7681 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     7741 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     7801 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     7861 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     7921 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     7981 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8041 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8101 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     8161 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     8221 ttgacattgc tagcgtttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     8281 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8341 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8401 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8461 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8521 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8581 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8641 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8701 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8761 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8821 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8881 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8941 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9001 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9061 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     9121 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9181 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9241 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9301 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9361 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9421 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9481 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9541 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9601 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9661 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9721 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9781 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9841 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9901 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9961 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10021 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10081 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10141 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10201 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10261 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10321 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10381 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
    10441 cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    10501 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    10561 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
    10621 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
    10681 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
    10741 aacttagtag tttttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
