LOCUS       V041581                10250 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041581
VERSION     V041581
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2829..2849
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             2850..4253
                     /label="Gsdmc(NM_031378)"
                     /note="Gsdmc(NM_031378)"
                     /gene="Gsdmc"
     CDS             4290..5009
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        5038..5537
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5558..6157
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6171..6759
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6966..7599
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7728..7744)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7752..7768
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7776..7806)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7821..7842
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8130..8718)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8889..9749)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9750..9854)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9902..10036
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgcc caagaagaag aggaaggtga tgtcctacac atttgactgg cttagcaagg
     2881 atgtggttaa aaagcttcaa ggaagagatc tgaggcctgt caaatgcctg tcagatgcaa
     2941 ccaaattctg cctgtttaac atacttcagg aaacttcttc ccggttggct ttgaaaactg
     3001 aatatattcc agttggattt acccttctgc atctcttgga gcccaatatt cctgttccag
     3061 agcctgaagt gtcagctccc atccccctta aacacaccat atcccagaaa ctgaaggctg
     3121 acctggatgt cgaaaccatt gcaggaggag aagcagggtt tgttaagtcc tgtggatatg
     3181 acatcgaggt tcagagtaag agcatcccaa atccaaaact ggaaagtctt caaaacagaa
     3241 aactgttgga tcagttgccc acatttatga agacttgctg gaaggatgga aagaacctgt
     3301 atgtggtgac agaggcctat gaagtgacca aggatactgt gctggaaggc acaagcaata
     3361 gcaaatttgc aatcaaaggg atcatcaacc agcttgtcaa ggttggaggc agtggacagt
     3421 ggcaaacaga gaaaacagat tcaataccta tccaaaaggg ttcagtgttg gcctacaaga
     3481 agcagcaatt ggtcattgag gataacactt gtgttattct cacttctgct aacactaaga
     3541 agaagatgac ctttccaatg agatttgtgg gaatgtctgg acatctaaga tatcaggatc
     3601 tagtcattga gacaggctcg tggataaatg acatcgatcc aattggaaca attaaggagc
     3661 ccacccatct agatttcatg tgcctacaaa atgaagtttc tgaacaaaca cggctactgg
     3721 ctgaactctc aaaggatgtt caagaagttg tgttctccag tttcctgcac atgctctgtg
     3781 acagggatgt tctgtatgac ctgatgaaaa tgctggagtt gaatcagttg gggcatatgg
     3841 atggcccagg tggcaaaatc ctggatgagc ttcgaaagga ctcgagcctt tcatggatta
     3901 acctaaagga cctcattctt tatctccttc aagccctgat ggtgctgagt gatacccaac
     3961 tctgtctgct ggccctgtct gtggagatgc ggctcctccc gcaccaagtg gagctggtaa
     4021 agagcatctt acagccaaac ttcaagtatc cctggaacat tcccttcaca ctccagcctc
     4081 agcttcttgc cccactccag ggtgagggct tggccattac ttatgaattg ctggaggagt
     4141 gtggtctgaa gatggagctg aataatccca ggtcaacttg ggatttggaa gccaagatgc
     4201 ccctgtctgc tctctatgga agcctgtcat ttttacagca actttcagaa gccggtggag
     4261 gcggatcagg aggcggtgga tcgggcccga tggtgagcaa gggcgaggag ctgttcaccg
     4321 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     4381 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     4441 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     4501 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     4561 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     4621 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     4681 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     4741 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     4801 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     4861 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     4921 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     4981 ctctcggcat ggacgagctg tacaagtagg gatcccgcga ctctagataa ttctaccggg
     5041 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     5101 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     5161 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     5221 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     5281 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     5341 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     5401 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     5461 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     5521 catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt
     5581 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     5641 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     5701 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     5761 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     5821 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     5881 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     5941 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6001 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     6061 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     6121 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc
     6181 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6241 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6301 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6361 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6421 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6481 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6541 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     6601 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6661 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6721 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
     6781 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     6841 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     6901 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag
     6961 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     7021 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     7081 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     7141 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     7201 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     7261 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     7321 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     7381 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     7441 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7501 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7561 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     7621 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
     7681 gctagcgttt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     7741 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7801 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7861 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7921 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7981 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8041 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8101 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8161 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8221 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8281 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8341 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8401 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8461 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8521 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8581 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8641 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8701 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8761 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8821 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8881 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8941 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9001 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9061 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9121 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9181 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9241 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9301 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9361 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9421 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9481 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9541 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9601 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9661 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9721 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9781 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9841 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     9901 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9961 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    10021 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    10081 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
    10141 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
    10201 agtttttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
