LOCUS       V027139                10939 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027139
VERSION     V027139
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..3542
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3573..5636
                     /label="Grk3(NM_177078)"
                     /note="Grk3(NM_177078)"
                     /gene="Grk3"
     CDS             5643..5666
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     promoter        5727..6226
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6247..6846
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6860..7448
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7655..8288
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8417..8433)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8441..8457
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8465..8495)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8510..8531
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8819..9407)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9578..10438)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10439..10543)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10591..10725
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2881 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2941 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3001 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3061 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3121 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3181 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3241 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     3301 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3361 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3421 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     3481 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3541 agggcggtgg aggcagtggt ggaggcggta gcatggcgga cctggaggcc gtgctggccg
     3601 acgtcagcta cctgatggcc atggagaaga gcaagacggc gccggccgcg cgcgccagca
     3661 agaaggtcgt cctgccggag cccagtatcc ggagtgtgat gcagaggtat ctggcagaga
     3721 gaaacgaaat aacctttgac aagattttca atcagaaaat cggcttccta ctatttaaag
     3781 acttctgctt gaatgaaatt ggggaagcag tgcctcaggt gaaattttat gaagagataa
     3841 aagaatatga gaagctggac aacgaagagg ataggctacg ccggagccgg cagatgtacg
     3901 acgcctacat catgagggag ctcctatcca gcacacacca attctcaaag caagctgtag
     3961 aacatgtcca gagccatctc tccaagaaac aggtgacggc tacacttttc cagccataca
     4021 tagaagaaat ctgtgaaagc cttcgtgggg atattttcca aaagtttatg gaaagcgata
     4081 aattcactag attctgtcag tggaagaacg tggagttgaa tattcacctg agcatgaacg
     4141 acttcagtgt gcacaggatc atcggccgag gagggtttgg ggaagtttat ggctgcagga
     4201 aagcagacac cggtaaaatg tatgccatga agtgcttaga caagaaaagg gtgaagatga
     4261 agcaggggga gactctggct ttgaacgaga ggatcatgct gtctctcgtt agcactgggg
     4321 attgtccctt tattgtctgc atgacctacg ccttccacac gccggacaaa ctctgcttca
     4381 tcctggacct gatgaacggg ggcgacatgc actaccatct ctctcaacac ggggtgtttt
     4441 ctgagaagga gatgcggttt tatgccagcg agatcatcct gggcctcgag cacatgcaca
     4501 cctgcttcgt agtctacaga gacctgaagc ctgcgaacat cctcctagat gaatatgggc
     4561 acgtgaggat atcggatctc ggccttgcct gcgatttctc caaaaagaag cctcatgcca
     4621 gcgtgggcac ccatgggtac atggctcccg aggtgttgca gaagggaacg tgctatgaca
     4681 gcagcgccga ctggttctcc ctgggctgta tgctcttcaa gcttctgcgg ggccacagcc
     4741 ccttcaggca gcataaaacc aaagacaagc atgagataga ccgaatgacc ctgaccgtga
     4801 acgtgcagct tccagatgcc ttctcccctg agctgaggtc cctcttagag ggtttgctcc
     4861 agcgggacgt gagccagcgg ctgggctgcg gaggaggagg ggcacgagag ttgaaggagc
     4921 acatcttctt caagggcatt gactggcagc atgtgtactt acggaagtac ccgccacccc
     4981 taatccctcc tcggggagag gtcaacgctg cagatgcctt cgatatcggc tccttcgatg
     5041 aggaagacac caaaggcatt aagctgttgg actgtgacca ggacctctat aagaacttcc
     5101 cactggtgat ctccgagcgc tggcagcaag aagtggtgga gaccatctat gacgccgtca
     5161 atgctgatac tgataaaatc gaggccagga ggaaggctaa aaataagcaa cttggtcaag
     5221 aggaagatta cgctatgggg aaggactgca tcatgcacgg gtacatgctg aagctgggga
     5281 acccctttct cacacagtgg caaagacgct atttttacct gttccccaac agactggagt
     5341 ggagaggaga gggcgagtct cggcaaagtc tactgaccat ggaacagatc atgtctgtgg
     5401 aggagaccca gattaaagac agaaagtgca tcttactcag gataaaggga gggaagcaat
     5461 ttgtcttgca atgtgagagt gaccccgagt ttgcacagtg gctgaaggag ctgacctgca
     5521 ccttcaatga ggcccagaga ctgctgcgcc gtgcccccaa attcctcaac aaaccacggg
     5581 ccgccatcct ggagttctcc aagccaccac tgtgtcacag aaatagcagc ggcctcgaat
     5641 tcgattacaa ggatgacgac gataagtgaa ttctgcagtc gacggtaccg cgggcccggg
     5701 atcccgcgac tctagataat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     5761 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     5821 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     5881 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     5941 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     6001 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     6061 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     6121 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     6181 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag
     6241 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6301 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6361 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6421 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6481 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6541 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6601 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6661 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6721 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6781 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6841 gcctgaccgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6901 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6961 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7021 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7081 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7141 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7201 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7261 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7321 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7381 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7441 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt
     7501 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     7561 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     7621 gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga
     7681 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     7741 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     7801 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     7861 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     7921 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     7981 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     8041 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     8101 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8161 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     8221 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8281 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     8341 aatatcagag agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga
     8401 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8461 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8521 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8581 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8641 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8701 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8761 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8821 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8881 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8941 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9001 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9061 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9121 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9181 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9241 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9301 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9361 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9421 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9481 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9541 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9601 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9661 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9721 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9781 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9841 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9901 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9961 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10021 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10081 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10141 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10201 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10261 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10321 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10381 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10441 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10501 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10561 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
    10621 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    10681 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    10741 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    10801 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    10861 atttgttaaa tatgtactac aaacttagta gtttttaaag aaattgtatt tgttaaatat
    10921 gtactacaaa cttagtagt
//
