LOCUS V045471 10975 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045471 VERSION V045471 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..5662 /label="GALNT5(NM_014568)" /note="GALNT5(NM_014568)" /gene="GALNT5" CDS 5678..5743 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 5763..6262 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 6283..6882 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6896..7484 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7691..8324 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(8453..8469) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8477..8493 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8501..8531) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8546..8567 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8855..9443) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9614..10474) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10475..10579) /gene="bla" /label="AmpR promoter" polyA_signal 10627..10761 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgaacag gatccgaaag tttttccgag gaagtgggcg 2881 agtcttggca tttatctttg tagcttctgt catctggctc ctctttgaca tggcagctct 2941 ccgcctctca ttcagtgaga tcaacactcg ggtcatcaag gaagacattg tgaggaggga 3001 gcggatagga ttcagagttc agccagacca aggaaaaatt ttttacagca gcataaaaga 3061 gatgaaacct cccctaaggg gacatgggaa aggggcatgg ggcaaagaga atgttagaaa 3121 aactgaggag agtgtgctca aggttgaggt ggacttggac caaacccaga gggaaagaaa 3181 aatgcagaat gccctgggaa ggggcaaggt tgtgccgttg tggcatcctg cacatctgca 3241 gaccctccct gtgactccta acaagcagaa gacagacggg agaggcacca aacctgaagc 3301 ctcctctcac caggggacac caaagcaaac gacagctcag ggggctccaa agacctcatt 3361 catagcagca aaaggaactc aggtagtcaa aatatcagta cacatgggac gtgtcagttt 3421 aaaacaggag ccccggaaga gtcatagtcc cagcagtgac acatcaaaac tagcagctga 3481 aagggacttg aatgtgacca tcagtcttag tactgataga ccaaagcagc gatcacaggc 3541 agtagcaaac gagagggcac accctgccag cacagcagtg ccgaagtctg gggaagccat 3601 ggccttaaac aaaactaaga ctcagagcaa agaagtcaat gcaaataaac acaaagccaa 3661 tacgagtctt ccttttccta agttcactgt caattcaaat cgcttaagga agcaatctat 3721 taatgagaca cctttgggaa gtttgtcaaa ggatgatgga gctagagggg ctcatgggaa 3781 gaaactcaat ttctctgaaa gccatcttgt gattataacc aaagaggaag agcaaaaggc 3841 agaccccaaa gaggtctcta attctaaaac caaaacaata tttcctaaag tattgggtaa 3901 aagccaaagt aaacacattt ccaggaatag aagtgagatg tcttcctctt cacttgctcc 3961 acatagagtg ccactgtccc aaactaacca tgctttaact ggagggctag agccagcaaa 4021 aatcaacata actgccaaag ccccctctac agaatacaac cagagtcata taaaagccct 4081 tttacctgaa gacagtggaa cgcaccaggt gttaagaatt gatgtgacac tttctccaag 4141 ggaccccaaa gctccagggc agtttgggcg tcctgtagtt gtcccccatg gaaaggagaa 4201 ggaggcagaa agaagatgga aagaaggaaa cttcaatgtc taccttagcg atttgatccc 4261 agtggataga gccattgaag acaccagacc tgctggatgt gcagagcagc tagttcacaa 4321 taacctccca accaccagtg tcatcatgtg ctttgtggat gaagtgtggt ccactctcct 4381 gagatctgtt cacagtgtca tcaatcgctc tcctccacac ctcatcaagg agattctgct 4441 ggtagatgac ttcagcacca aagactatct aaaagataat ttggataaat acatgtccca 4501 gtttccaaaa gttcggattc ttcgcctcaa agagagacat ggcttaataa gggccaggct 4561 ggcaggagca cagaatgcaa caggtgatgt gttgacattt ttagattctc atgtggaatg 4621 taacgttggt tggttggaac ctcttctgga aagagtttat ttaagtagaa agaaagtggc 4681 ctgtccagta atcgaagtca tcaatgataa ggatatgagt tacatgacag tggataactt 4741 tcaaagaggc atctttgtgt ggcccatgaa ctttggttgg agaacaattc ctccagatgt 4801 cattgcaaaa aacagaatta aagaaactga tacaataagg tgccctgtca tggctggtgg 4861 attgttttct attgacaaaa gttacttttt tgaacttgga acatacgacc ctggccttga 4921 tgtttggggt ggggaaaata tggagctctc attcaaggtg tggatgtgtg gtggtgaaat 4981 tgagatcatt ccctgctccc gagtgggcca tatattcaga aatgacaatc catattcctt 5041 ccccaaagac cggatgaaga cagtggagcg gaacttggtg cgggttgccg aggtctggct 5101 ggatgagtat aaggagctgt tctatggcca cggagaccac ctcatcgacc aagggctaga 5161 tgttggcaac ctcacccagc aaagggagct gcgaaagaaa ctgaagtgca aaagtttcaa 5221 atggtacttg gagaatgtct ttcctgactt aagggctccc attgtgagag ctagtggtgt 5281 gcttattaat gtggctttgg gtaaatgcat ttccattgaa aacactacag tcattctgga 5341 agactgcgat gggagcaaag agcttcaaca atttaattac acctggttaa gacttattaa 5401 atgtggagaa tggtgtatag cccccatccc tgataaagga gccgtaaggc tgcacccttg 5461 tgataacaga aacaaagggc taaaatggct gcataaatca acatcagtct ttcatccaga 5521 actggtgaat cacattgttt ttgaaaacaa tcagcaatta ttatgcttgg aaggaaattt 5581 ttctcaaaag atcctgaaag tagctgcctg tgacccagtg aagccatatc aaaagtggaa 5641 atttgaaaaa tattatgaag ccggtggagg cggatccgac tacaaagacc atgacggtga 5701 ttataaagat catgacatcg actacaagga tgacgatgac aagtagtcta gataattcta 5761 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct 5821 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag 5881 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt 5941 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc 6001 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct 6061 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag 6121 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc 6181 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc 6241 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc 6301 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 6361 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 6421 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 6481 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 6541 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 6601 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 6661 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 6721 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 6781 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 6841 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct ggaacaatca 6901 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 6961 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 7021 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 7081 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 7141 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 7201 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 7261 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 7321 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 7381 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 7441 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc 7501 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct 7561 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag 7621 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 7681 aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg 7741 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc 7801 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta 7861 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg 7921 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac 7981 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag 8041 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga 8101 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga 8161 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 8221 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 8281 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 8341 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg 8401 acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 8461 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 8521 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 8581 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 8641 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 8701 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 8761 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 8821 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 8881 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 8941 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 9001 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 9061 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 9121 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 9181 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 9241 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 9301 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 9361 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 9421 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 9481 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 9541 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 9601 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 9661 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 9721 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 9781 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 9841 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 9901 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 9961 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 10021 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 10081 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 10141 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 10201 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 10261 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 10321 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 10381 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 10441 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 10501 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 10561 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 10621 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 10681 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 10741 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt 10801 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt 10861 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac 10921 ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt //