LOCUS       V033752                10424 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033752
VERSION     V033752
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2838..4496
                     /label="FOXC1(NM_001453)"
                     /note="FOXC1(NM_001453)"
                     /gene="FOXC1"
     CDS             4512..5231
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="Aequorea coerulescens GFP"
                     /transl_table=1
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK*"
                     /label="AcGFP1"
     promoter        5259..5758
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5779..6378
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6392..6980
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7187..7820
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7949..7965)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7973..7989
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7997..8027)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8042..8063
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8351..8939)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9110..9970)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9971..10075)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10123..10257
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattctatg caggcgcgct actccgtgtc cagccccaac tccctgggag
     2881 tggtgcccta cctcggcggc gagcagagct actaccgcgc ggcggccgcg gcggccgggg
     2941 gcggctacac cgccatgccg gcccccatga gcgtgtactc gcaccctgcg cacgccgagc
     3001 agtacccggg cggcatggcc cgcgcctacg ggccctacac gccgcagccg cagcccaagg
     3061 acatggtgaa gccgccctat agctacatcg cgctcatcac catggccatc cagaacgccc
     3121 cggacaagaa gatcaccctg aacggcatct accagttcat catggaccgc ttccccttct
     3181 accgggacaa caagcagggc tggcagaaca gcatccgcca caacctctcg ctcaacgagt
     3241 gcttcgtcaa ggtgccgcgc gacgacaaga agccgggcaa gggcagctac tggacgctgg
     3301 acccggactc ctacaacatg ttcgagaacg gcagcttcct gcggcggcgg cggcgcttca
     3361 agaagaagga cgcggtgaag gacaaggagg agaaggacag gctgcacctc aaggagccgc
     3421 ccccgcccgg ccgccagccc ccgcccgcgc cgccggagca ggccgacggc aacgcgcccg
     3481 gtccgcagcc gccgcccgtg cgcatccagg acatcaagac cgagaacggt acgtgcccct
     3541 cgccgcccca gcccctgtcc ccggccgccg ccctgggcag cggcagcgcc gccgcggtgc
     3601 ccaagatcga gagccccgac agcagcagca gcagcctgtc cagcgggagc agccccccgg
     3661 gcagcctgcc gtcggcgcgg ccgctcagcc tggacggtgc ggattccgcg ccgccgccgc
     3721 ccgcgccctc cgccccgccg ccgcaccata gccagggctt cagcgtggac aacatcatga
     3781 cgtcgctgcg ggggtcgccg cagagcgcgg ccgcggagct cagctccggc cttctggcct
     3841 cggcggccgc gtcctcgcgc gcggggatcg cacccccgct ggcgctcggc gcctactcgc
     3901 ccggccagag ctccctctac agctccccct gcagccagac ctccagcgcg ggcagctcgg
     3961 gcggcggcgg cggcggcgcg ggggccgcgg ggggcgcggg cggcgccggg acctaccact
     4021 gcaacctgca agccatgagc ctgtacgcgg ccggcgagcg cgggggccac ttgcagggcg
     4081 cgcccggggg cgcgggcggc tcggccgtgg acgaccccct gcccgactac tctctgcctc
     4141 cggtcaccag cagcagctcg tcgtccctga gtcacggcgg cggcggcggc ggcggcgggg
     4201 gaggccagga ggccggccac caccctgcgg cccaccaagg ccgcctcacc tcgtggtacc
     4261 tgaaccaggc gggcggagac ctgggccact tggcgagcgc ggcggcggcg gcggcggccg
     4321 caggctaccc gggccagcag cagaacttcc actcggtgcg ggagatgttc gagtcacaga
     4381 ggatcggctt gaacaactct ccagtgaacg ggaatagtag ctgtcaaatg gccttccctt
     4441 ccagccagtc tctgtaccgc acgtccggag ctttcgtcta cgactgtagc aagtttccag
     4501 caccagcacc aatggtgagc aagggcgccg agctgttcac cggcatcgtg cccatcctga
     4561 tcgagctgaa tggcgatgtg aatggccaca agttcagcgt gagcggcgag ggcgagggcg
     4621 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcctgtgc
     4681 cctggcccac cctggtgacc accctgagct acggcgtgca gtgcttctca cgctaccccg
     4741 atcacatgaa gcagcacgac ttcttcaaga gcgccatgcc tgagggctac atccaggagc
     4801 gcaccatctt cttcgaggat gacggcaact acaagtcgcg cgccgaggtg aagttcgagg
     4861 gcgataccct ggtgaatcgc atcgagctga ccggcaccga tttcaaggag gatggcaaca
     4921 tcctgggcaa taagatggag tacaactaca acgcccacaa tgtgtacatc atgaccgaca
     4981 aggccaagaa tggcatcaag gtgaacttca agatccgcca caacatcgag gatggcagcg
     5041 tgcagctggc cgaccactac cagcagaata cccccatcgg cgatggccct gtgctgctgc
     5101 ccgataacca ctacctgtcc acccagagcg ccctgtccaa ggaccccaac gagaagcgcg
     5161 atcacatgat ctacttcggc ttcgtgaccg ccgccgccat cacccacggc atggatgagc
     5221 tgtacaagtg agcggccgcg actctagata attctaccgg gtaggggagg cgcttttccc
     5281 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     5341 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     5401 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     5461 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     5521 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     5581 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     5641 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     5701 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     5761 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5821 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5881 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     5941 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6001 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6061 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6121 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6181 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6241 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6301 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6361 cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     6421 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6481 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6541 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6601 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6661 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6721 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6781 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6841 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6901 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6961 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     7021 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     7081 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     7141 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     7201 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     7261 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     7321 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     7381 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     7441 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     7501 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     7561 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     7621 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7681 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7741 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7801 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7861 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga
     7921 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7981 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8041 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8101 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8161 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8221 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8281 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8341 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8401 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8461 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8521 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8581 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8641 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8701 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8761 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8821 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8881 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8941 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9001 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9061 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9121 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9181 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9241 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9301 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9361 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9421 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9481 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9541 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9601 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9661 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9721 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9781 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9841 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9901 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9961 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10021 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10081 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
    10141 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    10201 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    10261 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    10321 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    10381 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
