LOCUS V044413 9481 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V044413 VERSION V044413 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..4216 /label="FBXW9(NM_032301)" /note="FBXW9(NM_032301)" /gene="FBXW9" CDS 4223..4249 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 4269..4768 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 4789..5388 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5402..5990 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6197..6830 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6959..6975) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6983..6999 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7007..7037) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7052..7073 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7361..7949) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8120..8980) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8981..9085) /gene="bla" /label="AmpR promoter" polyA_signal 9133..9267 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggagct tcccctaggg cggtgcgatg attcccgcac 2881 ctgggacgat gactcggacc cagagtcaga gacagaccca gacgcgcagg ccaaggccta 2941 cgtggcccgc gttctcagtc cgccaaaatc cgggctggcg ttctcgcgcc cctcgcagct 3001 atccacaccc gccgcgtccc cgagcgcttc ggagcctcgg gccgcgtcca gggtttcggc 3061 cgtaagtgag ccgggccttc tgagccttcc cccggagctg ctgctcgaga tctgctccta 3121 cctggacgcc cgcctcgtgc tccacgtcct gtcgcgggtg tgccacgcgc tccgcgacct 3181 cgtgtctgac catgtcacct ggaggctacg cgcgctacgc cgcgtacgcg cgccctaccc 3241 agtggtggaa gagaagaact ttgactggcc ggcagcctgc attgcgctgg agcagcacct 3301 gtcccgctgg gcagaggatg ggcgctgggt cgaatacttc tgcctggccg aaggccacgt 3361 ggcttccgtt gactcagtgc tgctgctcca gggtgggtca ctctgtctgt cgggctcccg 3421 agatcgcaac gtcaacttgt gggacctgcg gcagctgggg acggagtcca accaggttct 3481 gatcaagacc ttaggcacta agcgaaatag tacccatgag ggctgggtgt ggtcactggc 3541 agcgcaggac caccgcgtgt gctccggctc ctgggacagc acagtgaagc tctgggacat 3601 ggcagcggat gggcagcagt tcggcgagat aaaggccagc tcagccgtgc tgtgcctctc 3661 ctacctgcct gacatcctgg tgactggcac ctatgacaag aaggtgacca tctacgaccc 3721 cagagccggc ccagccctgt tgaagcacca gcaactacac tccagacccg tgctgaccct 3781 gctggcggat gaccggcaca tcatctcagg cagcgaggac cacaccctgg tggtggtgga 3841 ccgccgagcc aacagcgtcc tgcagcgtct gcagctggac tcctacctgc tctgcatgtc 3901 ctaccaggaa ccccagctct gggctggtga caaccagggc ctgctgcacg tcttcgccaa 3961 ccgcaacggc tgcttccagc ttatccggtc ctttgatgtg ggccacagct ttcccatcac 4021 tgggatccag tactccgtgg gagccttgta caccacatcc actgacaaga ccatccgggt 4081 gcacgtgccc acagacccac caaggaccat ttgcacccga aggcatgaca atgggctcaa 4141 tagggtctgt gctgagggca acctggtggt ggccggctct ggagacctgt cgctagaggt 4201 ctggaggctg caggccggat cctacccata cgacgtacca gattacgctt agtctagata 4261 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 4321 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 4381 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 4441 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 4501 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 4561 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 4621 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 4681 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 4741 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac 4801 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 4861 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 4921 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 4981 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 5041 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 5101 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 5161 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 5221 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 5281 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 5341 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa 5401 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 5461 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 5521 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 5581 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 5641 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 5701 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 5761 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 5821 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 5881 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 5941 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa 6001 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc 6061 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca 6121 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 6181 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat 6241 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca 6301 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat 6361 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat 6421 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt 6481 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc 6541 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag 6601 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg 6661 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 6721 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 6781 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 6841 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 6901 gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg taatcatggt 6961 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 7021 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 7081 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 7141 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 7201 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 7261 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 7321 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 7381 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 7441 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 7501 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 7561 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 7621 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 7681 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 7741 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 7801 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 7861 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 7921 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 7981 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 8041 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 8101 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 8161 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 8221 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 8281 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 8341 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 8401 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 8461 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 8521 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 8581 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 8641 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 8701 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 8761 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 8821 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 8881 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 8941 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 9001 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 9061 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg 9121 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 9181 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 9241 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc 9301 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa 9361 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact 9421 acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag 9481 t //