LOCUS V043981 8521 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V043981 VERSION V043981 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..3196 /label="ELOB(NM_007108)" /note="ELOB(NM_007108)" /gene="ELOB" CDS 3203..3229 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3233..3259 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3263..3289 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 3309..3808 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 3829..4428 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4442..5030 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5237..5870 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(5999..6015) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6023..6039 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6047..6077) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6092..6113 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6401..6989) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7160..8020) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8021..8125) /gene="bla" /label="AmpR promoter" polyA_signal 8173..8307 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggacgt gttcctcatg atccggcgcc acaagaccac 2881 catcttcacg gacgccaagg agtccagcac ggtgttcgaa ctgaagcgca tcgtcgaggg 2941 catcctcaag cggcctcctg acgagcagcg gctgtacaag gatgaccaac tcttggatga 3001 tggcaagaca ctgggcgagt gtggcttcac cagtcaaaca gcacggccac aggccccagc 3061 cacagtgggg ctggccttcc gggcagatga cacctttgag gccctgtgca tcgagccgtt 3121 ttccagcccg ccagagctgc ccgatgtgat gaagccccag gactcgggaa gcagtgccaa 3181 tgaacaagcc gtgcagggat cctacccata cgacgtacct gattatgcaa cttatcctta 3241 tgatgtacct gattatgcta gctatcctta tgatgtccca gactacgcat agtctagata 3301 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 3361 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 3421 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 3481 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 3541 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 3601 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 3661 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 3721 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 3781 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac 3841 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 3901 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 3961 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 4021 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 4081 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 4141 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 4201 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 4261 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 4321 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 4381 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa 4441 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 4501 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 4561 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 4621 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 4681 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 4741 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 4801 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 4861 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 4921 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 4981 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa 5041 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc 5101 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca 5161 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 5221 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat 5281 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca 5341 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat 5401 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat 5461 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt 5521 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc 5581 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag 5641 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg 5701 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 5761 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 5821 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 5881 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 5941 gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg taatcatggt 6001 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 6061 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 6121 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 6181 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 6241 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 6301 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 6361 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 6421 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 6481 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 6541 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 6601 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 6661 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 6721 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 6781 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 6841 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 6901 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 6961 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 7021 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 7081 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 7141 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 7201 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 7261 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 7321 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 7381 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 7441 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 7501 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 7561 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 7621 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 7681 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 7741 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 7801 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 7861 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 7921 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 7981 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 8041 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 8101 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg 8161 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 8221 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 8281 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc 8341 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa 8401 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact 8461 acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag 8521 t //