LOCUS       V019233                11345 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019233
VERSION     V019233
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..5416
                     /label="EEF2(NM_001961)"
                     /note="EEF2(NM_001961)"
                     /gene="EEF2"
     CDS             5433..6152
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="Aequorea coerulescens GFP"
                     /transl_table=1
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK*"
                     /label="AcGFP1"
     promoter        6180..6679
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6700..7299
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7313..7901
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8108..8741
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8870..8886)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8894..8910
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8918..8948)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8963..8984
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9272..9860)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10031..10891)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10892..10996)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11044..11178
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatggtgaa cttcacggta gaccagatcc gcgccatcat
     2881 ggacaagaag gccaacatcc gcaacatgtc tgtcatcgcc cacgtggacc atggcaagtc
     2941 cacgctgaca gactccctgg tgtgcaaggc gggcatcatc gcctcggccc gggccgggga
     3001 gacacgcttc actgataccc ggaaggacga gcaggagcgt tgcatcacca tcaagtcaac
     3061 tgccatctcc ctcttctacg agctctcgga gaatgacttg aacttcatca agcagagcaa
     3121 ggacggtgcc ggcttcctca tcaacctcat tgactccccc gggcatgtcg acttctcctc
     3181 ggaggtgact gctgccctcc gagtcaccga tggcgcattg gtggtggtgg actgcgtgtc
     3241 aggcgtgtgc gtgcagacgg agacagtgct gcggcaggcc attgccgagc gcatcaagcc
     3301 tgtgctgatg atgaacaaga tggaccgcgc cctgctggag ctgcagctgg agcccgagga
     3361 gctctaccag actttccagc gcatcgtgga gaacgtgaac gtcatcatct ccacctacgg
     3421 cgagggcgag agcggcccca tgggcaacat catgatcgat cctgtcctcg gtaccgtggg
     3481 ctttgggtct ggcctccacg ggtgggcctt caccctgaag cagtttgccg agatgtatgt
     3541 ggccaagttc gccgccaagg gggagggcca gttggggcct gccgagcggg ccaagaaagt
     3601 agaggacatg atgaagaagc tgtggggtga caggtacttt gacccagcca acggcaagtt
     3661 cagcaagtca gccaccagcc ccgaagggaa gaagctgcca cgcaccttct gccagctgat
     3721 cctggacccc atcttcaagg tgtttgatgc gatcatgaat ttcaagaaag aggagacagc
     3781 aaaactgata gagaaactgg acatcaaact ggacagcgag gacaaggaca aagaaggcaa
     3841 acccctgctg aaggctgtga tgcgccgctg gctgcctgcc ggagacgcct tgttgcagat
     3901 gatcaccatc cacctgccct cccctgtgac ggcccagaag taccgctgcg agctcctgta
     3961 cgaggggccc ccggacgacg aggctgccat gggcattaaa agctgtgacc ccaaaggccc
     4021 tcttatgatg tatatttcca aaatggtgcc aacctccgac aaaggtcggt tctacgcctt
     4081 tggacgagtc ttctcggggc tggtctccac tggcctgaag gtcaggatca tggggcccaa
     4141 ctatacccct gggaagaagg aggacctcta cctgaagcca atccagagaa caatcttgat
     4201 gatgggccgc tacgtggagc ccatcgagga tgtgccttgt gggaacattg tgggcctcgt
     4261 gggcgtggac cagttcctgg tgaagacggg caccatcacc accttcgagc acgcgcacaa
     4321 catgcgggtg atgaagttca gcgtcagccc tgttgtcaga gtggccgtgg aggccaagaa
     4381 cccggctgac ctgcccaagc tggtggaggg gctgaagcgg ctggccaagt ccgaccccat
     4441 ggtgcagtgc atcatcgagg agtcgggaga gcatatcatc gcgggcgccg gcgagctgca
     4501 cctggagatc tgcctgaagg acctggagga ggaccacgcc tgcatcccca tcaagaaatc
     4561 tgacccggtc gtctcgtacc gcgagacggt cagtgaagag tcgaacgtgc tctgcctctc
     4621 caagtccccc aacaagcaca accggctgta catgaaggcg cggcccttcc ccgacggcct
     4681 ggccgaggac atcgataaag gcgaggtgtc cgcccgtcag gagctcaagc agcgggcgcg
     4741 ctacctggcc gagaagtacg agtgggacgt ggctgaggcc cgcaagatct ggtgctttgg
     4801 gcccgacggc accggcccca acatcctcac cgacatcacc aagggtgtgc agtacctcaa
     4861 cgagatcaag gacagtgtgg tggccggctt ccagtgggcc accaaggagg gcgcactgtg
     4921 tgaggagaac atgcggggtg tgcgcttcga cgtccacgac gtcaccctgc acgccgacgc
     4981 catccaccgc ggagggggcc agatcatccc cacagcacgg cgctgcctct atgccagtgt
     5041 gctgaccgcc cagccacgcc tcatggagcc catctacctt gtggagatcc agtgtccaga
     5101 gcaggtggtc ggtggcatct acggggtttt gaacaggaag cggggccacg tgttcgagga
     5161 gtcccaggtg gccggcaccc ccatgtttgt ggtcaaggcc tatctgcccg tcaacgagtc
     5221 ctttggcttc accgctgacc tgaggtccaa cacgggcggc caggcgttcc cccagtgtgt
     5281 gtttgaccac tggcagatcc tgcccggaga ccccttcgac aacagcagcc gccccagcca
     5341 ggtggtggcg gagacccgca agcgcaaggg cctgaaagaa ggcatccctg ccctggacaa
     5401 cttcctggac aaattgtagg gatccaccgg tcatggtgag caagggcgcc gagctgttca
     5461 ccggcatcgt gcccatcctg atcgagctga atggcgatgt gaatggccac aagttcagcg
     5521 tgagcggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     5581 ccaccggcaa gctgcctgtg ccctggccca ccctggtgac caccctgagc tacggcgtgc
     5641 agtgcttctc acgctacccc gatcacatga agcagcacga cttcttcaag agcgccatgc
     5701 ctgagggcta catccaggag cgcaccatct tcttcgagga tgacggcaac tacaagtcgc
     5761 gcgccgaggt gaagttcgag ggcgataccc tggtgaatcg catcgagctg accggcaccg
     5821 atttcaagga ggatggcaac atcctgggca ataagatgga gtacaactac aacgcccaca
     5881 atgtgtacat catgaccgac aaggccaaga atggcatcaa ggtgaacttc aagatccgcc
     5941 acaacatcga ggatggcagc gtgcagctgg ccgaccacta ccagcagaat acccccatcg
     6001 gcgatggccc tgtgctgctg cccgataacc actacctgtc cacccagagc gccctgtcca
     6061 aggaccccaa cgagaagcgc gatcacatga tctacttcgg cttcgtgacc gccgccgcca
     6121 tcacccacgg catggatgag ctgtacaagt gagcggccgc gactctagat aattctaccg
     6181 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     6241 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     6301 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     6361 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     6421 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     6481 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     6541 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     6601 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     6661 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
     6721 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     6781 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     6841 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6901 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6961 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     7021 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     7081 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     7141 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     7201 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     7261 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc
     7321 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     7381 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     7441 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     7501 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     7561 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     7621 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     7681 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     7741 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     7801 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7861 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt
     7921 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     7981 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     8041 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     8101 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     8161 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     8221 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     8281 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     8341 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     8401 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     8461 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     8521 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     8581 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8641 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8701 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     8761 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca
     8821 ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     8881 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8941 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     9001 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     9061 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     9121 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     9181 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9241 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9301 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9361 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9421 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9481 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9541 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9601 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9661 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     9721 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9781 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9841 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9901 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9961 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10021 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    10081 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10141 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10201 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10261 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10321 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10381 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10441 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10501 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10561 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10621 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10681 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10741 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10801 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10861 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10921 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10981 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
    11041 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    11101 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    11161 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
    11221 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
    11281 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    11341 gtagt
//
