LOCUS V038567 11083 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038567 VERSION V038567 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..5140 /label="DPP4(NM_001935)" /note="DPP4(NM_001935)" /gene="DPP4" CDS 5171..5890 /codon_start=1 /note="mammalian codon-optimized" /product="Aequorea coerulescens GFP" /transl_table=1 /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF GFVTAAAITHGMDELYK*" /label="AcGFP1" promoter 5918..6417 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 6438..7037 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7051..7639 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7846..8479 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(8608..8624) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8632..8648 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8656..8686) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8701..8722 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9010..9598) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9769..10629) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10630..10734) /gene="bla" /label="AmpR promoter" polyA_signal 10782..10916 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgaagac accgtggaag gttcttctgg gactgctggg 2881 tgctgctgcg cttgtcacca tcatcaccgt gcccgtggtt ctgctgaaca aaggcacaga 2941 tgatgctaca gctgacagtc gcaaaactta cactctaact gattacttaa aaaatactta 3001 tagactgaag ttatactcct taagatggat ttcagatcat gaatatctct acaaacaaga 3061 aaataatatc ttggtattca atgctgaata tggaaacagc tcagttttct tggagaacag 3121 tacatttgat gagtttggac attctatcaa tgattattca atatctcctg atgggcagtt 3181 tattctctta gaatacaact acgtgaagca atggaggcat tcctacacag cttcatatga 3241 catttatgat ttaaataaaa ggcagctgat tacagaagag aggattccaa acaacacaca 3301 gtgggtcaca tggtcaccag tgggtcataa attggcatat gtttggaaca atgacattta 3361 tgttaaaatt gaaccaaatt taccaagtta cagaatcaca tggacgggga aagaagatat 3421 aatatataat ggaataactg actgggttta tgaagaggaa gtcttcagtg cctactctgc 3481 tctgtggtgg tctccaaacg gcactttttt agcatatgcc caatttaacg acacagaagt 3541 cccacttatt gaatactcct tctactctga tgagtcactg cagtacccaa agactgtacg 3601 ggttccatat ccaaaggcag gagctgtgaa tccaactgta aagttctttg ttgtaaatac 3661 agactctctc agctcagtca ccaatgcaac ttccatacaa atcactgctc ctgcttctat 3721 gttgataggg gatcactact tgtgtgatgt gacatgggca acacaagaaa gaatttcttt 3781 gcagtggctc aggaggattc agaactattc ggtcatggat atttgtgact atgatgaatc 3841 cagtggaaga tggaactgct tagtggcacg gcaacacatt gaaatgagta ctactggctg 3901 ggttggaaga tttaggcctt cagaacctca ttttaccctt gatggtaata gcttctacaa 3961 gatcatcagc aatgaagaag gttacagaca catttgctat ttccaaatag ataaaaaaga 4021 ctgcacattt attacaaaag gcacctggga agtcatcggg atagaagctc taaccagtga 4081 ttatctatac tacattagta atgaatataa aggaatgcca ggaggaagga atctttataa 4141 aatccaactt agtgactata caaaagtgac atgcctcagt tgtgagctga atccggaaag 4201 gtgtcagtac tattctgtgt cattcagtaa agaggcgaag tattatcagc tgagatgttc 4261 cggtcctggt ctgcccctct atactctaca cagcagcgtg aatgataaag ggctgagagt 4321 cctggaagac aattcagctt tggataaaat gctgcagaat gtccagatgc cctccaaaaa 4381 actggacttc attattttga atgaaacaaa attttggtat cagatgatct tgcctcctca 4441 ttttgataaa tccaagaaat atcctctact attagatgtg tatgcaggcc catgtagtca 4501 aaaagcagac actgtcttca gactgaactg ggccacttac cttgcaagca cagaaaacat 4561 tatagtagct agctttgatg gcagaggaag tggttaccaa ggagataaga tcatgcatgc 4621 aatcaacaga agactgggaa catttgaagt tgaagatcaa attgaagcag ccagacaatt 4681 ttcaaaaatg ggatttgtgg acaacaaacg aattgcaatt tggggctggt catatggagg 4741 gtacgtaacc tcaatggtcc tgggatcggg aagtggcgtg ttcaagtgtg gaatagccgt 4801 ggcgcctgta tcccggtggg agtactatga ctcagtgtac acagaacgtt acatgggtct 4861 cccaactcca gaagacaacc ttgaccatta cagaaattca acagtcatga gcagagctga 4921 aaattttaaa caagttgagt acctccttat tcatggaaca gcagatgata acgttcactt 4981 tcagcagtca gctcagatct ccaaagccct ggtcgatgtt ggagtggatt tccaggcaat 5041 gtggtatact gatgaagacc atggaatagc tagcagcaca gcacaccaac atatatatac 5101 ccacatgagc cacttcataa aacaatgttt ctctttacct ggcggcggag ggagtacgga 5161 tccaccggtc atggtgagca agggcgccga gctgttcacc ggcatcgtgc ccatcctgat 5221 cgagctgaat ggcgatgtga atggccacaa gttcagcgtg agcggcgagg gcgagggcga 5281 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcctgtgcc 5341 ctggcccacc ctggtgacca ccctgagcta cggcgtgcag tgcttctcac gctaccccga 5401 tcacatgaag cagcacgact tcttcaagag cgccatgcct gagggctaca tccaggagcg 5461 caccatcttc ttcgaggatg acggcaacta caagtcgcgc gccgaggtga agttcgaggg 5521 cgataccctg gtgaatcgca tcgagctgac cggcaccgat ttcaaggagg atggcaacat 5581 cctgggcaat aagatggagt acaactacaa cgcccacaat gtgtacatca tgaccgacaa 5641 ggccaagaat ggcatcaagg tgaacttcaa gatccgccac aacatcgagg atggcagcgt 5701 gcagctggcc gaccactacc agcagaatac ccccatcggc gatggccctg tgctgctgcc 5761 cgataaccac tacctgtcca cccagagcgc cctgtccaag gaccccaacg agaagcgcga 5821 tcacatgatc tacttcggct tcgtgaccgc cgccgccatc acccacggca tggatgagct 5881 gtacaagtga gcggccgcga ctctagataa ttctaccggg taggggaggc gcttttccca 5941 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc 6001 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt 6061 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag 6121 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg 6181 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt 6241 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg 6301 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg 6361 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc 6421 tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 6481 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 6541 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 6601 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg 6661 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 6721 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 6781 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc 6841 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 6901 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 6961 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc 7021 gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa aatttgtgaa 7081 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 7141 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 7201 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 7261 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 7321 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 7381 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 7441 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg 7501 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 7561 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 7621 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag 7681 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag 7741 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca 7801 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc 7861 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc 7921 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt 7981 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca 8041 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag 8101 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt 8161 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag 8221 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag 8281 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 8341 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 8401 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 8461 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 8521 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt taccgtcgac 8581 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 8641 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 8701 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 8761 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 8821 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 8881 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 8941 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 9001 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 9061 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 9121 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 9181 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 9241 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 9301 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 9361 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 9421 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 9481 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 9541 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 9601 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 9661 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 9721 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 9781 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 9841 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 9901 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 9961 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 10021 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 10081 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 10141 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 10201 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 10261 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 10321 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 10381 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 10441 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 10501 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 10561 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 10621 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 10681 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 10741 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt 10801 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 10861 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact 10921 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg 10981 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt 11041 aaagaaattg tatttgttaa atatgtacta caaacttagt agt //