LOCUS V018408 11405 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018408 VERSION V018408 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" misc_feature 2811..2867 /note="multiple cloning site" /label="MCS" CDS 2874..3350 /EC_number="" /codon_start=1 /experiment="" /function="" /gene="folA" /gene_synonym="" /locus_tag="" /note="" /product="E. coli dihydrofolate reductase" /protein_id="" /transl_table=1 /translation="MISLIAALAVDHVIGMETVMPWNLPADLAWFKRNTLNKPVIMGRH TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFL PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR" /label="ecDHFR" CDS 3393..6128 /codon_start=1 /product="DNA methyltransferase 3A" /transl_table=1 /translation="MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTA RKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEG SPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGKEQKETNIESMKM EGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIA GMNAVEENQGPGESQKVEEASPPAVQQPTDPASPTVATTPEPVGSDAGDKNATKAGDDE PEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVC VEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPVCHDSDESDTAKAV EVQNKPMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPR KSTAEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFVGGMCQNC KNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAI KEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIR VLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKH IQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFW LFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDK LELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFP VHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV" /label="DNMT3A" CDS 6147..6173 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 6193..6692 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 6713..7312 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7326..7914 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 8121..8754 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(8883..8899) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8907..8923 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8931..8961) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8976..8997 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9285..9873) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(10044..10904) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10905..11009) /gene="bla" /label="AmpR promoter" polyA_signal 11057..11191 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccgcc accatgatca 2881 gtctgattgc ggcgttagcg gtagatcacg ttatcggcat ggaaaccgtc atgccgtgga 2941 acctgcctgc cgatctcgcc tggtttaaac gcaacacctt aaataaaccc gtgattatgg 3001 gccgccatac ctgggaatca atcggtcgtc cgttgccagg acgcaaaaat attatcctca 3061 gcagtcaacc gagtacggac gatcgcgtaa cgtgggtgaa gtcggtggat gaagccatcg 3121 cggcgtgtgg tgacgtacca gaaatcatgg ttattggcgg cggtcgcgtt tatgaacagt 3181 tcttgccaaa agcgcaaaaa ctgtatctga cgcatatcga cgcagaagtg gaaggcgaca 3241 cccatttccc ggattacgag ccggatgact gggaatcggt attcagcgaa ttccacgatg 3301 ctgatgcgca gaactctcac agctattgct ttgagattct ggagcggcga ggcggcggag 3361 ggagtggagg agggggaagc aagcttggta ccatgcccgc tatgccctcc agcggccccg 3421 gggacaccag cagctctgct gcggagcggg aggaggaccg aaaggacgga gaggagcagg 3481 aggagccgcg tggcaaggag gagcgccaag agcccagcac cacggcacgg aaggtggggc 3541 ggcctgggag gaagcgcaag caccccccgg tggaaagcgg tgacacgcca aaggaccctg 3601 cggtgatctc caagtcccca tccatggccc aggactcagg cgcctcagag ctattaccca 3661 atggggactt ggagaagcgg agtgagcccc agccagagga ggggagccct gctggggggc 3721 agaagggcgg ggccccagca gagggagagg gtgcagctga gaccctgcct gaagcctcaa 3781 gagcagtgga aaatggctgc tgcaccccca aggagggccg aggagcccct gcagaagcgg 3841 gcaaagaaca gaaggagacc aacatcgaat ccatgaaaat ggagggctcc cggggccggc 3901 tgcggggtgg cttgggctgg gagtccagcc tccgtcagcg gcccatgccg aggctcacct 3961 tccaggcggg ggacccctac tacatcagca agcgcaagcg ggacgagtgg ctggcacgct 4021 ggaaaaggga ggctgagaag aaagccaagg tcattgcagg aatgaatgct gtggaagaaa 4081 accaggggcc cggggagtct cagaaggtgg aggaggccag ccctcctgct gtgcagcagc 4141 ccactgaccc cgcatccccc actgtggcta ccacgcctga gcccgtgggg tccgatgctg 4201 gggacaagaa tgccaccaaa gcaggcgatg acgagccaga gtacgaggac ggccggggct 4261 ttggcattgg ggagctggtg tgggggaaac tgcggggctt ctcctggtgg ccaggccgca 4321 ttgtgtcttg gtggatgacg ggccggagcc gagcagctga aggcacccgc tgggtcatgt 4381 ggttcggaga cggcaaattc tcagtggtgt gtgttgagaa gctgatgccg ctgagctcgt 4441 tttgcagtgc gttccaccag gccacgtaca acaagcagcc catgtaccgc aaagccatct 4501 acgaggtcct gcaggtggcc agcagccgcg cggggaagct gttcccggtg tgccacgaca 4561 gcgatgagag tgacactgcc aaggccgtgg aggtgcagaa caagcccatg attgaatggg 4621 ccctgggggg cttccagcct tctggcccta agggcctgga gccaccagaa gaagagaaga 4681 atccctacaa agaagtgtac acggacatgt gggtggaacc tgaggcagct gcctacgcac 4741 cacctccacc agccaaaaag ccccggaaga gcacagcgga gaagcccaag gtcaaggaga 4801 ttattgatga gcgcacaaga gagcggctgg tgtacgaggt gcggcagaag tgccggaaca 4861 ttgaggacat ctgcatctcc tgtgggagcc tcaatgttac cctggaacac cccctcttcg 4921 ttggaggaat gtgccaaaac tgcaagaact gctttctgga gtgtgcgtac cagtacgacg 4981 acgacggcta ccagtcctac tgcaccatct gctgtggggg ccgtgaggtg ctcatgtgcg 5041 gaaacaacaa ctgctgcagg tgcttttgcg tggagtgtgt ggacctcttg gtggggccgg 5101 gggctgccca ggcagccatt aaggaagacc cctggaactg ctacatgtgc gggcacaagg 5161 gtacctacgg gctgctgcgg cggcgagagg actggccctc ccggctccag atgttcttcg 5221 ctaataacca cgaccaggaa tttgaccctc caaaggttta cccacctgtc ccagctgaga 5281 agaggaagcc catccgggtg ctgtctctct ttgatggaat cgctacaggg ctcctggtgc 5341 tgaaggactt gggcattcag gtggaccgct acattgcctc ggaggtgtgt gaggactcca 5401 tcacggtggg catggtgcgg caccagggga agatcatgta cgtcggggac gtccgcagcg 5461 tcacacagaa gcatatccag gagtggggcc cattcgatct ggtgattggg ggcagtccct 5521 gcaatgacct ctccatcgtc aaccctgctc gcaagggcct ctacgagggc actggccggc 5581 tcttctttga gttctaccgc ctcctgcatg atgcgcggcc caaggaggga gatgatcgcc 5641 ccttcttctg gctctttgag aatgtggtgg ccatgggcgt tagtgacaag agggacatct 5701 cgcgatttct cgagtccaac cctgtgatga ttgatgccaa agaagtgtca gctgcacaca 5761 gggcccgcta cttctggggt aaccttcccg gtatgaacag gccgttggca tccactgtga 5821 atgataagct ggagctgcag gagtgtctgg agcatggcag gatagccaag ttcagcaaag 5881 tgaggaccat tactacgagg tcaaactcca taaagcaggg caaagaccag cattttcctg 5941 tcttcatgaa tgagaaagag gacatcttat ggtgcactga aatggaaagg gtatttggtt 6001 tcccagtcca ctatactgac gtctccaaca tgagccgctt ggcgaggcag agactgctgg 6061 gccggtcatg gagcgtgcca gtcatccgcc acctcttcgc tccgctgaag gagtattttg 6121 cgtgtgtgtg cgggggtgga ggctcttatc cttacgacgt gcctgactac gcctaatcta 6181 gataattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag 6241 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat 6301 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc 6361 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa 6421 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc 6481 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga 6541 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg 6601 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc 6661 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga 6721 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct 6781 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat 6841 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 6901 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 6961 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 7021 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 7081 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 7141 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 7201 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 7261 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct 7321 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 7381 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 7441 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 7501 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 7561 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 7621 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 7681 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 7741 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 7801 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 7861 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa 7921 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc 7981 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt 8041 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 8101 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct 8161 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg 8221 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc 8281 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct 8341 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc 8401 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc 8461 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg 8521 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc 8581 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 8641 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 8701 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 8761 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 8821 agaggccttg acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca 8881 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 8941 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 9001 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 9061 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 9121 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 9181 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 9241 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 9301 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 9361 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 9421 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 9481 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 9541 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 9601 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 9661 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 9721 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 9781 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 9841 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 9901 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 9961 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 10021 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 10081 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 10141 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 10201 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 10261 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 10321 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 10381 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 10441 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 10501 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 10561 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 10621 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 10681 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 10741 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 10801 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 10861 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 10921 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 10981 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 11041 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag 11101 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 11161 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca 11221 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact 11281 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg 11341 tactacaaac ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta 11401 gtagt //