LOCUS       V018408                11405 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018408
VERSION     V018408
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     misc_feature    2811..2867
                     /note="multiple cloning site"
                     /label="MCS"
     CDS             2874..3350
                     /EC_number=""
                     /codon_start=1
                     /experiment=""
                     /function=""
                     /gene="folA"
                     /gene_synonym=""
                     /locus_tag=""
                     /note=""
                     /product="E. coli dihydrofolate reductase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MISLIAALAVDHVIGMETVMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
                     /label="ecDHFR"
     CDS             3393..6128
                     /codon_start=1
                     /product="DNA methyltransferase 3A"
                     /transl_table=1
                     /translation="MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTA
                     RKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEG
                     SPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGKEQKETNIESMKM
                     EGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIA
                     GMNAVEENQGPGESQKVEEASPPAVQQPTDPASPTVATTPEPVGSDAGDKNATKAGDDE
                     PEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVC
                     VEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPVCHDSDESDTAKAV
                     EVQNKPMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPR
                     KSTAEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFVGGMCQNC
                     KNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAI
                     KEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIR
                     VLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKH
                     IQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFW
                     LFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDK
                     LELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFP
                     VHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV"
                     /label="DNMT3A"
     CDS             6147..6173
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        6193..6692
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6713..7312
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7326..7914
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8121..8754
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8883..8899)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8907..8923
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8931..8961)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8976..8997
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9285..9873)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10044..10904)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10905..11009)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11057..11191
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccgcc accatgatca
     2881 gtctgattgc ggcgttagcg gtagatcacg ttatcggcat ggaaaccgtc atgccgtgga
     2941 acctgcctgc cgatctcgcc tggtttaaac gcaacacctt aaataaaccc gtgattatgg
     3001 gccgccatac ctgggaatca atcggtcgtc cgttgccagg acgcaaaaat attatcctca
     3061 gcagtcaacc gagtacggac gatcgcgtaa cgtgggtgaa gtcggtggat gaagccatcg
     3121 cggcgtgtgg tgacgtacca gaaatcatgg ttattggcgg cggtcgcgtt tatgaacagt
     3181 tcttgccaaa agcgcaaaaa ctgtatctga cgcatatcga cgcagaagtg gaaggcgaca
     3241 cccatttccc ggattacgag ccggatgact gggaatcggt attcagcgaa ttccacgatg
     3301 ctgatgcgca gaactctcac agctattgct ttgagattct ggagcggcga ggcggcggag
     3361 ggagtggagg agggggaagc aagcttggta ccatgcccgc tatgccctcc agcggccccg
     3421 gggacaccag cagctctgct gcggagcggg aggaggaccg aaaggacgga gaggagcagg
     3481 aggagccgcg tggcaaggag gagcgccaag agcccagcac cacggcacgg aaggtggggc
     3541 ggcctgggag gaagcgcaag caccccccgg tggaaagcgg tgacacgcca aaggaccctg
     3601 cggtgatctc caagtcccca tccatggccc aggactcagg cgcctcagag ctattaccca
     3661 atggggactt ggagaagcgg agtgagcccc agccagagga ggggagccct gctggggggc
     3721 agaagggcgg ggccccagca gagggagagg gtgcagctga gaccctgcct gaagcctcaa
     3781 gagcagtgga aaatggctgc tgcaccccca aggagggccg aggagcccct gcagaagcgg
     3841 gcaaagaaca gaaggagacc aacatcgaat ccatgaaaat ggagggctcc cggggccggc
     3901 tgcggggtgg cttgggctgg gagtccagcc tccgtcagcg gcccatgccg aggctcacct
     3961 tccaggcggg ggacccctac tacatcagca agcgcaagcg ggacgagtgg ctggcacgct
     4021 ggaaaaggga ggctgagaag aaagccaagg tcattgcagg aatgaatgct gtggaagaaa
     4081 accaggggcc cggggagtct cagaaggtgg aggaggccag ccctcctgct gtgcagcagc
     4141 ccactgaccc cgcatccccc actgtggcta ccacgcctga gcccgtgggg tccgatgctg
     4201 gggacaagaa tgccaccaaa gcaggcgatg acgagccaga gtacgaggac ggccggggct
     4261 ttggcattgg ggagctggtg tgggggaaac tgcggggctt ctcctggtgg ccaggccgca
     4321 ttgtgtcttg gtggatgacg ggccggagcc gagcagctga aggcacccgc tgggtcatgt
     4381 ggttcggaga cggcaaattc tcagtggtgt gtgttgagaa gctgatgccg ctgagctcgt
     4441 tttgcagtgc gttccaccag gccacgtaca acaagcagcc catgtaccgc aaagccatct
     4501 acgaggtcct gcaggtggcc agcagccgcg cggggaagct gttcccggtg tgccacgaca
     4561 gcgatgagag tgacactgcc aaggccgtgg aggtgcagaa caagcccatg attgaatggg
     4621 ccctgggggg cttccagcct tctggcccta agggcctgga gccaccagaa gaagagaaga
     4681 atccctacaa agaagtgtac acggacatgt gggtggaacc tgaggcagct gcctacgcac
     4741 cacctccacc agccaaaaag ccccggaaga gcacagcgga gaagcccaag gtcaaggaga
     4801 ttattgatga gcgcacaaga gagcggctgg tgtacgaggt gcggcagaag tgccggaaca
     4861 ttgaggacat ctgcatctcc tgtgggagcc tcaatgttac cctggaacac cccctcttcg
     4921 ttggaggaat gtgccaaaac tgcaagaact gctttctgga gtgtgcgtac cagtacgacg
     4981 acgacggcta ccagtcctac tgcaccatct gctgtggggg ccgtgaggtg ctcatgtgcg
     5041 gaaacaacaa ctgctgcagg tgcttttgcg tggagtgtgt ggacctcttg gtggggccgg
     5101 gggctgccca ggcagccatt aaggaagacc cctggaactg ctacatgtgc gggcacaagg
     5161 gtacctacgg gctgctgcgg cggcgagagg actggccctc ccggctccag atgttcttcg
     5221 ctaataacca cgaccaggaa tttgaccctc caaaggttta cccacctgtc ccagctgaga
     5281 agaggaagcc catccgggtg ctgtctctct ttgatggaat cgctacaggg ctcctggtgc
     5341 tgaaggactt gggcattcag gtggaccgct acattgcctc ggaggtgtgt gaggactcca
     5401 tcacggtggg catggtgcgg caccagggga agatcatgta cgtcggggac gtccgcagcg
     5461 tcacacagaa gcatatccag gagtggggcc cattcgatct ggtgattggg ggcagtccct
     5521 gcaatgacct ctccatcgtc aaccctgctc gcaagggcct ctacgagggc actggccggc
     5581 tcttctttga gttctaccgc ctcctgcatg atgcgcggcc caaggaggga gatgatcgcc
     5641 ccttcttctg gctctttgag aatgtggtgg ccatgggcgt tagtgacaag agggacatct
     5701 cgcgatttct cgagtccaac cctgtgatga ttgatgccaa agaagtgtca gctgcacaca
     5761 gggcccgcta cttctggggt aaccttcccg gtatgaacag gccgttggca tccactgtga
     5821 atgataagct ggagctgcag gagtgtctgg agcatggcag gatagccaag ttcagcaaag
     5881 tgaggaccat tactacgagg tcaaactcca taaagcaggg caaagaccag cattttcctg
     5941 tcttcatgaa tgagaaagag gacatcttat ggtgcactga aatggaaagg gtatttggtt
     6001 tcccagtcca ctatactgac gtctccaaca tgagccgctt ggcgaggcag agactgctgg
     6061 gccggtcatg gagcgtgcca gtcatccgcc acctcttcgc tccgctgaag gagtattttg
     6121 cgtgtgtgtg cgggggtgga ggctcttatc cttacgacgt gcctgactac gcctaatcta
     6181 gataattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     6241 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     6301 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     6361 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     6421 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     6481 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     6541 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     6601 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     6661 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga
     6721 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     6781 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     6841 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6901 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6961 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     7021 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     7081 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     7141 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     7201 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     7261 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct
     7321 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7381 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7441 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7501 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7561 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7621 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7681 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     7741 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7801 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7861 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     7921 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     7981 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     8041 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8101 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct
     8161 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     8221 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     8281 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     8341 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     8401 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     8461 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     8521 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     8581 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8641 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8701 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     8761 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8821 agaggccttg acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca
     8881 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8941 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     9001 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9061 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9121 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9181 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9241 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9301 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9361 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9421 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9481 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9541 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9601 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9661 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9721 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9781 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9841 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9901 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9961 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10021 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    10081 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10141 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10201 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10261 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10321 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10381 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10441 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10501 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10561 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10621 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10681 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10741 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10801 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10861 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10921 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10981 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    11041 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    11101 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    11161 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    11221 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
    11281 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
    11341 tactacaaac ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    11401 gtagt
//
