LOCUS       V026928                10557 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026928
VERSION     V026928
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2821..2830
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2830..2853
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             2854..5313
                     /label="DDX23(NM_004818)"
                     /note="DDX23(NM_004818)"
                     /gene="DDX23"
     promoter        5345..5844
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5865..6464
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6478..7066
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7273..7906
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8035..8051)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8059..8075
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8083..8113)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8128..8149
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8437..9025)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9196..10056)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10057..10161)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10209..10343
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccgccatgg actacaagga cgacgatgac aagatggcag gagagctggc tgacaaaaag
     2881 gaccgtgatg catcaccttc caaggaggaa aggaagcgat cacggactcc tgacagagag
     2941 cgggatagag accgggaccg gaagtcttcc ccatctaaag atagaaagcg gcatcgttca
     3001 agggatagac gtcgaggagg cagccgttct cgctctcgtt cccgttccaa atctgcagaa
     3061 agagaacgac ggcacaaaga acgagaacga gataaggagc gggatcggaa taagaaggac
     3121 cgagatcgag acaaggatgg gcacagacgg gacaaggacc gtaaacgatc cagcttatct
     3181 cctggtcgag gaaaagactt taaatctcgg aaggacagag actctaagaa ggatgaagag
     3241 gatgaacatg gtgataagaa gcctaaggcc cagccattat ccctggagga gcttctggcc
     3301 aagaaaaagg ctgaggaaga agctgaggct aagcccaagt tcctctctaa agcagaacga
     3361 gaggctgaag ctctaaagcg acggcagcag gaggtggaag agcggcagag gatgcttgaa
     3421 gaagagagga agaaaaggaa acagttccaa gacttgggca ggaagatgtt ggaagatcct
     3481 caggaacggg aacgtcggga acgcagggag aggatggaac gggagaccaa tggaaatgag
     3541 gatgaggaag ggcggcagaa gatccgggaa gagaaggata agagcaagga actgcatgcc
     3601 attaaggagc gttacctggg tggcatcaaa aagcggcgcc gaacgagaca tctcaatgac
     3661 cggaaatttg tttttgagtg ggatgcatct gaggacacat ccattgacta caaccccctg
     3721 tacaaagaac ggcaccaggt gcagttgtta gggcgaggct tcattgcagg cattgacctc
     3781 aagcagcaga agcgagagca gtcacgtttc tatggagacc taatggagaa gaggcgaacc
     3841 ctggaagaaa aggagcagga ggaggcaaga ctccgcaaac ttcgtaagaa ggaagccaag
     3901 cagcgctggg atgatcgtca ttggtctcag aaaaagttag atgagatgac ggacagggac
     3961 tggcggatct tccgtgagga ctacagcatc accaccaaag gtggcaagat ccccaatccc
     4021 atccgatcct ggaaagactc ttctctgccc ccacacatct tggaggtcat tgataagtgt
     4081 ggctacaagg aaccaacacc tatacagcgt caggcaattc ccattgggct acagaatcgt
     4141 gacatcattg gtgtggctga gactggcagt ggcaagacag cagccttcct catccctctg
     4201 ctggtctgga tcaccacact tcccaaaatt gacaggatcg aagagtcaga ccaaggccct
     4261 tatgccatca tcctggctcc cacccgtgag ttggctcaac agattgagga agagaccatc
     4321 aagtttggga aaccgctagg tatccgcact gtggctgtca ttggtggcat ctccagagaa
     4381 gaccagggct tcaggctgcg catgggttgt gagattgtga ttgctacccc tgggcgtttg
     4441 attgatgtgc tggagaaccg ctacctggtg ctgagccgct gtacctatgt ggttctggat
     4501 gaggcagata ggatgattga catgggcttt gagccagatg tccagaagat cctggagcac
     4561 atgcctgtca gcaaccagaa gccagacacg gatgaggctg aggaccctga gaagatgctg
     4621 gccaactttg agtcgggaaa acataagtac cgccaaacag tcatgttcac ggccaccatg
     4681 cccccagcgg tggagcgtct ggccaggagc tatcttcggc gacctgctgt ggtgtacatt
     4741 ggctccgcag gcaagcccca tgagcgtgtg gaacagaagg tcttcctcat gtcagagtca
     4801 gaaaagagga aaaagctgct ggcaatcttg gagcaaggct ttgacccacc catcattatt
     4861 tttgtcaacc agaagaaggg ctgcgacgtg ttggccaaat ccctggagaa gatggggtac
     4921 aatgcttgca cactgcacgg tggaaaaggc caggagcagc gagagtttgc gttgtccaac
     4981 ctcaaggctg gggccaagga tattttggtg gctacagatg tggctggtcg tggtattgac
     5041 atccaagatg tgtctatggt tgtcaactat gatatggcca aaaatattga agattacatc
     5101 caccgcattg gccgcacggg acgagcaggc aagagtgggg tggccatcac cttcctcaca
     5161 aaagaggact ctgctgtgtt ctacgagctg aagcaagcta tcctggaaag cccagtgtct
     5221 tcctgtcccc ccgaactagc caaccaccca gatgcccagc ataagccagg caccatcctc
     5281 accaagaagc gccgggaaga gaccatcttt gcctgaggat cccgcgactc tagataattc
     5341 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     5401 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     5461 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     5521 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     5581 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     5641 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     5701 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     5761 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     5821 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc
     5881 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     5941 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     6001 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     6061 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     6121 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     6181 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     6241 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     6301 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     6361 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     6421 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt ctggaacaat
     6481 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6541 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6601 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6661 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6721 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6781 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     6841 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     6901 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6961 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7021 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct
     7081 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     7141 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     7201 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7261 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     7321 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     7381 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     7441 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     7501 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     7561 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     7621 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     7681 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     7741 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7801 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7861 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     7921 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct
     7981 tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     8041 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8101 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8161 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8221 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8281 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8341 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8401 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8461 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8521 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8581 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8641 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8701 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8761 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8821 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8881 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8941 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9001 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9061 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9121 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9181 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9241 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9301 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9361 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9421 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9481 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9541 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9601 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9661 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9721 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9781 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9841 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9901 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9961 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10021 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10081 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10141 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    10201 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    10261 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    10321 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
    10381 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
    10441 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
    10501 acttagtagt ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt
//
