LOCUS V019592 9096 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019592 VERSION V019592 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2827..3828 /label="Cops5(NM_013715)" /note="Cops5(NM_013715)" /gene="Cops5" CDS 3829..3852 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" promoter 3884..4383 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 4404..5003 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5017..5605 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5812..6445 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6574..6590) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6598..6614 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6622..6652) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6667..6688 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6976..7564) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7735..8595) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8596..8700) /gene="bla" /label="AmpR promoter" polyA_signal 8748..8882 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 gccaccatgg cagcttccgg gagtggtatg gcccagaaaa cctgggaatt ggccaacaac 2881 atgcaggaag cgcagagtat cgatgaaatc tacaaatatg acaaaaaaca acaacaagaa 2941 atcctggcgg cgaaaccctg gactaaggat caccactact ttaaatactg caaaatctca 3001 gcattggctc tactgaaaat ggtgatgcat gccaggtcag gaggcaactt ggaagtgatg 3061 ggtttgatgc tcgggaaagt cgacggcgag accatgatca tcatggacag tttcgctttg 3121 cctgtagagg gcacagaaac tcgagtaaat gctcaagctg ctgcgtatga gtatatggct 3181 gcatacatag aaaatgccaa acaggttggc cgccttgaga atgcaatcgg ttggtatcat 3241 agccaccctg gttatggctg ctggctctcc gggattgatg ttagtacaca gatgctgaac 3301 cagcagtttc aagaaccatt tgtagcagtg gtgattgatc caaccagaac aatctctgca 3361 ggaaaagtga atcttggcgc ctttaggaca tatccaaagg gctacaaacc tcctgatgaa 3421 ggaccttctg agtaccagac tatcccactt aataaaatag aagattttgg cgtgcactgc 3481 aaacaatatt atgccttaga agtctcatat ttcaaatcat ctttggatcg taaactactt 3541 gagcttttgt ggaataaata ctgggtgaat accctgagtt cctctagctt gcttactaat 3601 gcagactaca ccacaggcca ggtgtttgat ttgtctgaga agttagagca gtcggaagcc 3661 caactgggac gtggcagttt catgttgggc ttagaaacac atgaccgcaa gtcggaagac 3721 aaacttgcca aagctactag agacagctgt aaaaccacca tagaagccat ccatggactg 3781 atgtctcagg ttattaagga taaactgttt aatcagatta acgttgctga ttacaaggac 3841 gacgatgaca agtagggatc ccgcgactct agataattct accgggtagg ggaggcgctt 3901 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca 3961 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc 4021 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc 4081 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg 4141 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag 4201 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg 4261 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc 4321 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt 4381 tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc 4441 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca 4501 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct 4561 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg 4621 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc 4681 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc 4741 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg 4801 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc 4861 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc 4921 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca 4981 tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga ttacaaaatt 5041 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 5101 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 5161 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 5221 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 5281 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 5341 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 5401 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg 5461 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 5521 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 5581 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac 5641 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag 5701 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga 5761 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc 5821 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct 5881 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg 5941 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag 6001 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag 6061 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc 6121 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact 6181 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat 6241 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag 6301 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 6361 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 6421 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta 6481 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta gcgttttacc 6541 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 6601 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 6661 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 6721 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 6781 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 6841 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 6901 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 6961 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 7021 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 7081 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 7141 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 7201 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 7261 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 7321 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 7381 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 7441 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7501 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 7561 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 7621 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 7681 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 7741 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 7801 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 7861 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 7921 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 7981 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8041 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 8101 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 8161 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 8221 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 8281 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 8341 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 8401 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 8461 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 8521 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 8581 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 8641 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 8701 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg 8761 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 8821 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 8881 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta 8941 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt 9001 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt tttaaagaaa 9061 ttgtatttgt taaatatgta ctacaaactt agtagt //