LOCUS       V021584                12143 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021584
VERSION     V021584
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..4466
                     /label="Cd19(NM_009844)"
                     /note="Cd19(NM_009844)"
                     /gene="Cd19"
     CDS             4482..5189
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEGYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5196..5249
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5253..6902
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
                     /label="luciferase"
     promoter        6931..7430
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             7451..8050
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8064..8652
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8859..9492
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9621..9637)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9645..9661
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9669..9699)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9714..9735
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10023..10611)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10782..11642)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11643..11747)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11795..11929
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgcc atctcctctc cctgtctcct tcctcctctt tcttacctta gtaggaggca
     2881 ggccccagaa gtccttactg gtggaggtag aagagggagg caatgttgtg ctgccatgcc
     2941 tcccggactc ctcacctgtc tcttctgaga agctggcttg gtatcgaggt aaccagtcaa
     3001 cacccttcct ggagctgagc cccgggtccc ctggcctggg attgcacgtg gggtccctgg
     3061 gcatcttgct agtgattgtc aatgtctcag accatatggg gggcttctac ctgtgccaga
     3121 agaggccccc tttcaaggac atctggcagc ctgcctggac agtgaacgtg gaggatagtg
     3181 gggagatgtt ccggtggaat gcttcagacg tcagggacct ggactgtgac ctaaggaaca
     3241 ggtcctctgg gagccacagg tccacttctg gttcccagct gtatgtgtgg gctaaagacc
     3301 atcctaaggt ctggggaaca aagcctgtat gtgcccctcg ggggagcagt ttgaatcaga
     3361 gtctaatcaa ccaagatctc actgtggcac ccggctccac actttggctg tcctgtgggg
     3421 taccccctgt cccagtggcc aaaggctcca tctcctggac ccatgtgcat cctaggagac
     3481 ctaatgtttc actactgagc ctaagccttg ggggagagca cccggtcaga gagatgtggg
     3541 tttgggggtc tcttctgctt ctgccccaag ccacagcttt agatgaaggc acctattatt
     3601 gtctccgagg aaacctgacc atcgagaggc acgtgaaggt cattgcaagg tcagcagtgt
     3661 ggctctggct gttgagaact ggtggatgga tagtcccagt ggtgacttta gtatatgtca
     3721 tcttctgtat ggtttctctg gtggcttttc tctattgtca aagagccttt atcctgagaa
     3781 ggaaaaggaa gcgaatgact gaccccgcca ggagattctt caaagtgacg cctccctcgg
     3841 gaaacgggac ccagaaccag tacgggaatg tgctctccct tcctacatct acctctggcc
     3901 aggcccatgc tcagcgttgg gctgctggcc tagggagtgt ccctgggtcc tatggaaatc
     3961 cacgcattca agtccaggat actggagctc agagccatga aacaggactg gaagaagaag
     4021 gggaggccta tgaagagcca gacagcgagg agggctctga attctatgag aacgactcca
     4081 accttgggca ggaccaggtt tcccaggatg ggagtggcta tgagaacccc gaggatgagc
     4141 ccatgggtcc agaggaagaa gactccttct ccaatgctga gtcttatgaa aatgcagatg
     4201 aggagctggc ccaaccagtt ggcaggatga tggacttcct gagcccccat gggtctgcgt
     4261 gggaccccag ccgggaagca tcctcgcttg ggtcccagtc ctatgaagat atgagaggga
     4321 tcctctatgc agctcctcag ctccactcaa ttcagtccgg tcccagtcat gaagaagatg
     4381 cagactctta tgaaaacatg gataagtctg acgacctaga accagcatgg gaaggagagg
     4441 gccacatggg gacttgggga accacgggag gagggggaag catggtgagc aagggcgagg
     4501 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     4561 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     4621 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     4681 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     4741 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     4801 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     4861 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     4921 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     4981 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     5041 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     5101 cctcccacaa cgagggctac accatcgtgg aacagtacga acgcgccgag ggccgccact
     5161 ccaccggcgg catggacgag ctgtacaagc cccgggaggg cagaggaagt cttctaacat
     5221 gcggtgacgt ggaggagaat cccggccctc gagaagacgc caaaaacata aagaaaggcc
     5281 cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga
     5341 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca
     5401 tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg
     5461 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc
     5521 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg
     5581 aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa
     5641 aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta
     5701 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc
     5761 atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga
     5821 caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc
     5881 ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa
     5941 tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta
     6001 ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag
     6061 agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc
     6121 tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg
     6181 aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt
     6241 tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca gctattctga
     6301 ttacgcccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag
     6361 cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt
     6421 gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct
     6481 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac
     6541 acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc
     6601 ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag
     6661 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa
     6721 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa
     6781 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg
     6841 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt
     6901 aaggatcccg cgactctaga taattctacc gggtagggga ggcgcttttc ccaaggcagt
     6961 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     7021 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     7081 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     7141 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     7201 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     7261 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     7321 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     7381 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     7441 caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     7501 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     7561 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     7621 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     7681 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     7741 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     7801 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     7861 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     7921 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     7981 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     8041 cggtgcctga ccgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga
     8101 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     8161 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     8221 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     8281 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     8341 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     8401 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     8461 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     8521 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     8581 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     8641 ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac
     8701 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     8761 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     8821 tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca
     8881 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
     8941 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
     9001 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
     9061 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
     9121 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
     9181 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
     9241 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
     9301 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9361 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9421 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9481 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     9541 aatgaatatc agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc
     9601 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     9661 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     9721 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9781 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     9841 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9901 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9961 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    10021 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    10081 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    10141 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    10201 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    10261 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    10321 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    10381 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    10441 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    10501 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    10561 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    10621 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    10681 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    10741 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    10801 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    10861 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    10921 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10981 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    11041 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    11101 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    11161 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    11221 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    11281 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    11341 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    11401 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    11461 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    11521 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    11581 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    11641 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    11701 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    11761 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg
    11821 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    11881 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    11941 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    12001 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
    12061 ttgtatttgt taaatatgta ctacaaactt agtagttttt aaagaaattg tatttgttaa
    12121 atatgtacta caaacttagt agt
//
