LOCUS       V013511                 9795 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013511
VERSION     V013511
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     sig_peptide     2827..2889
                     /label="Ig-kappa leader"
                     /note="leader sequence from mouse immunoglobulin kappa
                     light chain"
     CDS             2890..2916
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     promoter        4583..5082
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5103..5699
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    5716..6304
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6511..7144
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7273..7289)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7297..7313)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7321..7351)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7366..7387)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7675..8263)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8437..9294)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9295..9399)
                     /label="AmpR promoter"
     polyA_signal    9447..9581
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt tccaggttcc
     2881 actggtgact atccatatga tgttccagat tatgctgggg cccagccggc cgacatccag
     2941 atgacccagt ctccatcatc actgcctgcc tccctgggag acagagtcac tatcaattgt
     3001 caggccagtc aggacattag caattattta aactggtacc agcagaaacc agggaaagct
     3061 cctaagctcc tgatctatta tacaaataaa ttggcagatg gagtcccatc aaggttcagt
     3121 ggcagtggtt ctgggagaga ttcttctttc actatcagca gcctggaatc cgaagatatt
     3181 ggatcttatt actgtcaaca gtattataac tatccgtgga cgttcggacc tggcaccaag
     3241 ctggaaatca aacggggcag tacttcgggc gccggtaagt cgagtgaggg taagggtgag
     3301 gtgcagctgg tggagtctgg gggaggcttg gtgcagcctg gaaagtccct gaaactctcc
     3361 tgtgaggcct ctggattcac cttcagcggc tatggcatgc actgggtccg ccaggctcca
     3421 gggagggggc tggagtcggt cgcatacatt actagtagta gtattaatat caaatatgct
     3481 gacgctgtga aaggccggtt caccgtctcc agagacaatg ccaagaactt actgtttcta
     3541 caaatgaaca ttctcaagtc tgaggacaca gccatgtact actgtgcaag attcgactgg
     3601 gacaaaaatt actggggcca aggaaccatg gtcaccgtct cctcagtcga cgagcccaaa
     3661 tcttgtgaca aaactcacac atgcccaccg tgcccagcac ctgaactcct ggggggaccg
     3721 tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag
     3781 gtcacatgcg tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac
     3841 gtggacggcg tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc
     3901 acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag
     3961 tacaagtgca aggtctccaa caaagccctc ccagccccca tcgagaaaac catctccaaa
     4021 gccaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggatgagctg
     4081 accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctatcccag cgacatcgcc
     4141 gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg
     4201 gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag
     4261 caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacacag
     4321 aagagcctct ccctgtctcc gggtaaagtc gaccccccag aagaccctcc tgatagcaag
     4381 aacacacttg tgctctttgg ggcaggattc ggcgcagtaa taacagtcgt cgtcatcgtt
     4441 gtcatcatca aatgcttctg taagcacaga agctgtttca gaagaaatga ggcaagcaga
     4501 gaaacaaaca acagccttac cttcgggcct gaagaagcat tagctgaaca gaccgtcttc
     4561 ctttagtcta gataattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     4621 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     4681 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     4741 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     4801 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     4861 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     4921 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     4981 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     5041 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta
     5101 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     5161 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     5221 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     5281 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     5341 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     5401 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     5461 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     5521 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     5581 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     5641 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     5701 gaccgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5761 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5821 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5881 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5941 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6001 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6061 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     6121 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6181 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6241 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6301 ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca
     6361 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     6421 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     6481 ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac
     6541 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     6601 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     6661 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     6721 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     6781 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     6841 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     6901 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     6961 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7021 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7081 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7141 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7201 tcagagagtg agaggccttg acattgctag cgttttaccg tcgacctcta gctagagctt
     7261 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7321 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7381 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7441 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7501 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7561 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7621 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7681 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7741 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7801 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7861 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7921 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7981 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8041 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8101 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8161 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8221 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8281 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8341 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8401 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8461 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8521 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8581 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8641 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8701 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8761 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8821 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8881 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8941 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9001 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9061 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9121 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9181 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9241 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9301 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9361 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9421 acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat
     9481 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9541 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
     9601 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
     9661 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     9721 gttaaatatg tactacaaac ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac
     9781 tacaaactta gtagt
//
