LOCUS       V021249                12554 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021249
VERSION     V021249
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..7214
                     /label="CD163L1(NM_001297650)"
                     /note="CD163L1(NM_001297650)"
                     /gene="CD163L1"
     CDS             7215..7280
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7342..7841
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             7862..8461
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8475..9063
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9270..9903
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(10032..10048)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10056..10072
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10080..10110)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10125..10146
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10434..11022)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11193..12053)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12054..12158)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    12206..12340
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgat gctgcctcaa aactcgtggc atattgattt tggaagatgc tgctgtcatc
     2881 agaacctttt ctctgctgtg gtaacttgca tcctgctcct gaattcctgc tttctcatca
     2941 gcagttttaa tggaacagat ttggagttga ggctggtcaa tggagacggt ccctgctctg
     3001 ggacagtgga ggtgaaattc cagggacagt gggggactgt gtgtgatgat gggtggaaca
     3061 ctactgcctc aactgtcgtg tgcaaacagc ttggatgtcc attttctttc gccatgtttc
     3121 gttttggaca agccgtgact agacatggaa aaatttggct tgatgatgtt tcctgttatg
     3181 gaaatgagtc agctctctgg gaatgtcaac accgggaatg gggaagccat aactgttatc
     3241 atggagaaga tgttggtgtg aactgttatg gtaagagtct aactagagta ggaaggcaag
     3301 gtgaagccaa tctgggtttg aggctagtgg atggaaacaa ctcctgttca gggagagtgg
     3361 aggtgaaatt ccaagaaagg tggggaacta tatgtgatga tgggtggaac ttgaatactg
     3421 ctgccgtggt gtgcaggcaa ctaggatgtc catcttcttt tatttcttct ggagttgtta
     3481 atagccctgc tgtattgcgc cccatttggc tggatgacat tttatgccag gggaatgagt
     3541 tggcactctg gaattgcaga catcgtggat ggggaaatca tgactgcagt cacaatgagg
     3601 atgtcacatt aacttgttat gatagtagtg atcttgaact aaggcttgta ggtggaacta
     3661 accgctgtat ggggagagta gagctgaaaa tccaaggaag gtgggggacc gtatgccacc
     3721 ataagtggaa caatgctgca gctgatgtcg tatgcaagca gttgggatgt ggaaccgcac
     3781 ttcacttcgc tggcttgcct catttgcagt cagggtctga tgttgtatgg cttgatggtg
     3841 tctcctgctc cggtaatgaa tcttttcttt gggactgcag acattccgga accgtcaatt
     3901 ttgactgtct tcatcaaaac gatgtgtctg tgatctgctc agatggagca gatttggaac
     3961 tgcgactagc agatggaagt aacaattgtt cagggagagt agaggtgaga attcatgaac
     4021 agtggtggac aatatgtgac cagaactgga agaatgaaca agcccttgtg gtttgtaagc
     4081 agctaggatg tccgttcagc gtctttggca gtcgtcgtgc taaacctagt aatgaagcta
     4141 gagacatttg gataaacagc atatcttgca ctgggaatga gtcagctctc tgggactgca
     4201 catatgatgg aaaagcaaag cgaacatgct tccgaagatc agatgctgga gtaatttgtt
     4261 ctgataaggc agatctggac ctaaggcttg tcggggctca tagcccctgt tatgggagat
     4321 tggaggtgaa ataccaagga gagtggggga ctgtgtgtca tgacagatgg agcacaagga
     4381 atgcagctgt tgtttgtaaa caattgggat gtggaaagcc tttgcatgtg tttggtatga
     4441 cctattttaa agaagcatca ggacctattt ggctggatga cgtttcttgc attggaaatg
     4501 agtcaaatat ctgggactgt gaacacagtg gatggggaaa gcataattgt gtacacagag
     4561 aggatgtgat tgtaacctgc tcaggtgatg caacatgggg cctgaggctg gtgggcggca
     4621 gcaaccgctg ctcgggaaga ctggaggtgt actttcaagg acggtggggc acagtgtgtg
     4681 atgacggctg gaacagtaaa gctgcagctg tggtgtgtag ccagctggac tgcccatctt
     4741 ctatcattgg catgggtctg ggaaacgctt ctacaggata tggaaaaatt tggctcgatg
     4801 atgtttcctg tgatggagat gagtcagatc tctggtcatg caggaacagt gggtggggaa
     4861 ataatgactg cagtcacagt gaagatgttg gagtgatctg ttctgatgca tcggatatgg
     4921 agctgaggct tgtgggtgga agcagcaggt gtgctggaaa agttgaggtg aatgtccagg
     4981 gtgccgtggg aattctgtgt gctaatggct ggggaatgaa cattgctgaa gttgtttgca
     5041 ggcaacttga atgtgggtct gcaatcaggg tctccagaga gcctcatttc acagaaagaa
     5101 cattacacat cttaatgtcg aattctggct gcactggagg ggaagcctct ctctgggatt
     5161 gtatacgatg ggagtggaaa cagactgcgt gtcatttaaa tatggaagca agtttgatct
     5221 gctcagccca caggcagccc aggctggttg gagctgatat gccctgctct ggacgtgttg
     5281 aagtgaaaca tgcagacaca tggcgctctg tctgtgattc tgatttctct cttcatgctg
     5341 ccaatgtgct gtgcagagaa ttaaactgtg gagatgccat atctctttct gtgggagatc
     5401 actttggaaa agggaatggt ctaacttggg ccgaaaagtt ccagtgtgaa gggagtgaaa
     5461 ctcaccttgc attatgcccc attgttcaac atccggaaga cacttgtatc cacagcagag
     5521 aagttggagt tgtctgttcc cgatatacag atgtccgact tgtgaatggc aaatcccagt
     5581 gtgacgggca agtggagatc aacgtgcttg gacactgggg ctcactgtgt gacacccact
     5641 gggacccaga agatgcccgt gttctatgca gacagctcag ctgtgggact gctctctcaa
     5701 ccacaggagg aaaatatatt ggagaaagaa gtgttcgtgt gtggggacac aggtttcatt
     5761 gcttagggaa tgagtcactt ctggataact gtcaaatgac agttcttgga gcacctccct
     5821 gtatccatgg aaatactgtc tctgtgatct gcacaggaag cctgacccag ccactgtttc
     5881 catgcctcgc aaatgtatct gacccatatt tgtctgcagt tccagagggc agtgctttga
     5941 tctgcttaga ggacaaacgg ctccgcctag tggatgggga cagccgctgt gccgggagag
     6001 tagagatcta tcacgacggc ttctggggca ccatctgtga tgacggctgg gacctgagcg
     6061 atgcccacgt ggtgtgtcaa aagctgggct gtggagtggc cttcaatgcc acggtctctg
     6121 ctcactttgg ggaggggtca gggcccatct ggctggatga cctgaactgc acaggaatgg
     6181 agtcccactt gtggcagtgc ccttcccgcg gctgggggca gcacgactgc aggcacaagg
     6241 aggacgcagg ggtcatctgc tcagaattca cagccttgag gctctacagt gaaactgaaa
     6301 cagagagctg tgctgggaga ttggaagtct tctataacgg gacctggggc agcgtcggca
     6361 ggaggaacat caccacagcc atagcaggca ttgtgtgcag gcagctgggc tgtggggaga
     6421 atggagttgt cagcctcgcc cctttatcta agacaggctc tggtttcatg tgggtggatg
     6481 acattcagtg tcctaaaacg catatctcca tatggcagtg cctgtctgcc ccatgggagc
     6541 gaagaatctc cagcccagca gaagagacct ggatcacatg tgaagataga ataagagtgc
     6601 gtggaggaga caccgagtgc tctgggagag tggagatctg gcacgcaggc tcctggggca
     6661 cagtgtgtga tgactcctgg gacctggccg aggcggaagt ggtgtgtcag cagctgggct
     6721 gtggctctgc tctggctgcc ctgagggacg cttcgtttgg ccagggaact ggaaccatct
     6781 ggttggatga catgcggtgc aaaggaaatg agtcatttct atgggactgt cacgccaaac
     6841 cctggggaca gagtgactgt ggacacaagg aagatgctgg cgtgaggtgc tctggacagt
     6901 cgctgaaatc actgaatgcc tcctcaggtc atttagcact tattttatcc agtatctttg
     6961 ggctccttct cctggttctg tttattctat ttctcacgtg gtgccgagtt cagaaacaaa
     7021 aacatctgcc cctcagagtt tcaaccagaa ggaggggttc tctcgaggag aatttattcc
     7081 atgagatgga gacctgcctc aagagagagg acccacatgg gacaagaacc tcagatgaca
     7141 cccccaacca tggttgtgaa gatgctagcg acacatcgct gttgggagtt cttcctgcct
     7201 ctgaagccac aaaagactac aaagaccatg acggtgatta taaagatcat gacatcgact
     7261 acaaggatga cgatgacaag tagaattctg cagtcgacgg taccgcgggc ccgggatccc
     7321 gcgactctag ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     7381 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     7441 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     7501 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     7561 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     7621 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     7681 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     7741 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     7801 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     7861 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     7921 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     7981 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     8041 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     8101 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     8161 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     8221 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     8281 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     8341 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     8401 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     8461 accgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     8521 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     8581 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     8641 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     8701 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     8761 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     8821 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     8881 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     8941 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     9001 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     9061 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa
     9121 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     9181 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     9241 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca
     9301 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     9361 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     9421 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     9481 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     9541 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     9601 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     9661 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     9721 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     9781 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     9841 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     9901 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     9961 cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg
    10021 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    10081 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    10141 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    10201 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    10261 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    10321 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    10381 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    10441 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    10501 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    10561 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    10621 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    10681 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    10741 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10801 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    10861 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10921 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10981 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    11041 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    11101 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    11161 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    11221 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    11281 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    11341 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    11401 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    11461 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    11521 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    11581 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    11641 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    11701 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    11761 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    11821 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    11881 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    11941 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    12001 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    12061 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    12121 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    12181 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
    12241 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    12301 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    12361 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
    12421 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
    12481 ttaaatatgt actacaaact tagtagtttt taaagaaatt gtatttgtta aatatgtact
    12541 acaaacttag tagt
//
