LOCUS       V028506                10585 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028506
VERSION     V028506
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2821..4596
                     /label="CANX(NM_001746)"
                     /note="CANX(NM_001746)"
                     /gene="CANX"
     CDS             4612..5319
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK*"
                     /label="mCherry"
     promoter        5373..5872
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5893..6492
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6506..7094
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7301..7934
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8063..8079)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8087..8103
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8111..8141)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8156..8177
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8465..9053)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9224..10084)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10085..10189)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10237..10371
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatgccacc
     2821 atggaaggga agtggttgct gtgtatgtta ctggtgcttg gaactgctat tgttgaggct
     2881 catgatggac atgatgatga tgtgattgat attgaggatg accttgacga tgtcattgaa
     2941 gaggtagaag actcaaaacc agataccact gctcctcctt catctcccaa ggttacttac
     3001 aaagctccag ttccaacagg ggaagtatat tttgctgatt cttttgacag aggaactctg
     3061 tcagggtgga ttttatccaa agccaagaaa gacgataccg atgatgaaat tgccaaatat
     3121 gatggaaagt gggaggtaga ggaaatgaag gagtcaaagc ttccaggtga taaaggactt
     3181 gtgttgatgt ctcgggccaa gcatcatgcc atctctgcta aactgaacaa gcccttcctg
     3241 tttgacacca agcctctcat tgttcagtat gaggttaatt tccaaaatgg aatagaatgt
     3301 ggtggtgcct atgtgaaact gctttctaaa acaccagaac tcaacctgga tcagttccat
     3361 gacaagaccc cttatacgat tatgtttggt ccagataaat gtggagagga ctataaactg
     3421 cacttcatct tccgacacaa aaaccccaaa acgggtatct atgaagaaaa acatgctaag
     3481 aggccagatg cagatctgaa gacctatttt actgataaga aaacacatct ttacacacta
     3541 atcttgaatc cagataatag ttttgaaata ctggttgacc aatctgtggt gaatagtgga
     3601 aatctgctca atgacatgac tcctcctgta aatccttcac gtgaaattga ggacccagaa
     3661 gaccggaagc ccgaggattg ggatgaaaga ccaaaaatcc cagatccaga agctgtcaag
     3721 ccagatgact gggatgaaga tgcccctgct aagattccag atgaagaggc cacaaaaccc
     3781 gaaggctggt tagatgatga gcctgagtac gtacctgatc cagacgcaga gaaacctgag
     3841 gattgggatg aagacatgga tggagaatgg gaggctcctc agattgccaa ccctagatgt
     3901 gagtcagctc ctggatgtgg tgtctggcag cgacctgtga ttgacaaccc caattataaa
     3961 ggcaaatgga agcctcctat gattgacaat cccagttacc agggaatctg gaaacccagg
     4021 aaaataccaa atccagattt ctttgaagat ctggaacctt tcagaatgac tccttttagt
     4081 gctattggtt tggagctgtg gtccatgacc tctgacattt tttttgacaa ctttatcatt
     4141 tgtgctgatc gaagaatagt tgatgattgg gccaatgatg gatggggcct gaagaaagct
     4201 gctgatgggg ctgctgagcc aggcgttgtg gggcagatga tcgaggcagc tgaagagcgc
     4261 ccgtggctgt gggtagtcta tattctaact gtagcccttc ctgtgttcct ggttatcctc
     4321 ttctgctgtt ctggaaagaa acagaccagt ggtatggagt ataagaaaac tgatgcacct
     4381 caaccggatg tgaaggaaga ggaagaagag aaggaagagg aaaaggacaa gggagatgag
     4441 gaggaggaag gagaagagaa acttgaagag aaacagaaaa gtgatgctga agaagatggt
     4501 ggcactgtca gtcaagagga ggaagacaga aaacctaaag cagaggagga tgaaattttg
     4561 aacagatcac caagaaacag aaagccacga agagagggag gagggggaag cgtgagcaag
     4621 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     4681 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     4741 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     4801 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     4861 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     4921 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     4981 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     5041 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     5101 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     5161 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     5221 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     5281 cgccactcca ccggcggcat ggacgagctg tacaagtagt gcagtcgacg gtaccgcggg
     5341 cccgggatcc cgcgactcta gataattcta ccgggtaggg gaggcgcttt tcccaaggca
     5401 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     5461 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     5521 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     5581 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     5641 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     5701 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     5761 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     5821 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     5881 cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5941 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6001 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6061 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6121 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6181 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6241 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6301 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6361 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6421 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6481 cccggtgcct gaccgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt
     6541 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6601 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6661 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6721 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6781 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6841 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6901 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6961 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7021 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7081 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc
     7141 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     7201 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     7261 gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc
     7321 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     7381 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
     7441 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
     7501 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
     7561 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
     7621 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
     7681 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
     7741 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7801 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7861 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7921 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     7981 gaaatgaata tcagagagtg agaggccttg acattgctag cgttttaccg tcgacctcta
     8041 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     8101 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     8161 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     8221 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     8281 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8341 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8401 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8461 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8521 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8581 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8641 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8701 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8761 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8821 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8881 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     8941 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9001 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9061 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9121 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9181 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9241 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9301 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9361 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9421 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9481 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9541 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9601 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9661 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9721 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9781 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9841 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9901 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9961 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10021 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10081 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10141 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10201 gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat
    10261 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10321 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    10381 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    10441 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    10501 aattgtattt gttaaatatg tactacaaac ttagtagttt ttaaagaaat tgtatttgtt
    10561 aaatatgtac tacaaactta gtagt
//
