LOCUS V045164 10327 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045164 VERSION V045164 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..5002 /label="Acsl3(NM_028817)" /note="Acsl3(NM_028817)" /gene="Acsl3" CDS 5009..5035 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 5039..5065 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 5069..5095 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 5115..5614 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5635..6234 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6248..6836 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7043..7676 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7805..7821) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7829..7845 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7853..7883) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7898..7919 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8207..8795) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8966..9826) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9827..9931) /gene="bla" /label="AmpR promoter" polyA_signal 9979..10113 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgaataa ccacgtatct tcaacaccat caaccatgaa 2881 gctaaaacaa accatcaacc ccatactttt atatttcata cattttataa tatcactcta 2941 tactatttta acatacatcc cattttattt tttgtgtgag tcaaaacaag agaaaccaaa 3001 ccaaattaaa gcaaaacctg tcagttccaa accggactct gcatacagat ctatcaacag 3061 tgtggatggc ttggcttcag tgctgtatcc tggctgcgat acacttgata aagtctttat 3121 gtatgcaaaa aacaaattta aaaacaaaag actattggga acacgtgaaa ttttaaatga 3181 ggaagatgaa atacagccaa atggaaaaat ttttaaaaag gttattctgg ggcactataa 3241 ttggctttcc tatgaagatg tcttcatccg agcccttgac tttggaaatg ggttacaaat 3301 gttgggccag aaaccaaagg ccaacatcgc catcttctgt gagaccaggg ctgagtggat 3361 gatcgctgca caggcgtgtt ttatgtataa cttccagctt gttacactgt atgctactct 3421 gggaggtcca gccattgttc atggactgaa tgagacagag gtgaccaaca tcattactag 3481 caaagaactc ttgcaaacaa agctgaagga tatagtctct ttggtcccac gtctgcggca 3541 tatcattact gttgatggga agcctccaac ctggtctgag ttccccaaag gtgtcattgt 3601 acacaccatg gctgcagtgc aggctctagg agtgaaggcc aacgtggaaa agaaagctca 3661 cagcaaacca ctgccctcag atattgcagt aatcatgtac acaagtgggt ccacaggaat 3721 tccaaaggga gtcatgatct cacacagcaa catcattgct tctataacgg ggatggcgag 3781 aaggattcca agactgggag aggaagatgt gtatattggc tacttgcccc tggcacatgt 3841 tctagaatta agtgctgagc ttgtgtgtct ttctcatgga tgccgaatcg gctactcttc 3901 accacagaca ttagcagatc agtcttcaaa aataaaaaaa ggaagcaaag gagacacatc 3961 cgtgctgaag ccaacactga tggcagctgt tccggaaatc atggatcgga tctacaaaaa 4021 tgtcatgaat aaagtgaatg aaatgagtgc ttttcaacga aacttgttta ttttggcata 4081 taattataag atggaacaga tttcaaaagg gtgtagtact ccactgtgtg accgctttgt 4141 tttccggaat gtccgaaggc tgctgggtgg aaatattcgc cttttactgt gtggtggtgc 4201 tccgctttct gcaacaacgc agcgattcat gaatatctgt ttctgctgtc ccgttggtca 4261 ggggtatgga ctcacagaat ctactggggc tggaacaatt acagaagtgt gggactacaa 4321 taccggcaga gtgggagcac cattagtttg ctgtgaaatc aaattaaaga actgggagga 4381 aggtggctat tttaatactg acaaaccaca tcccagaggt gaaattctta ttggtggcca 4441 aaatgtgaca atggggtact acaaaaatga agcaaaaaca aagacagatt tctttgaaga 4501 tgaaaatgga cagcggtggc tgtgcactgg agatattgga gagtttgacc ctgacggctg 4561 tctgaagatc attgaccgta aaaaggacct tgtgaaacta caggcaggag agtacgtttc 4621 tctcgggaaa gtagaggcag ctttgaagaa cctcccactg atagataaca tttgtgcgta 4681 tgcaaacagc taccattctt acgtaattgg gtttgttgtg ccaaatcaaa aggaacttac 4741 agagctagct agaacaaaag gatttaaagg aacttgggaa gagctgtgta acagcagcga 4801 gatggaaaat gaggtcctta aagtgctttc tgaagctgct atttcagcaa gtctggaaaa 4861 gtttgaaatt ccactaaaaa tacgtttgag ccctgaccca tggactcccg aaactggtct 4921 ggtgacagat gccttcaagt tgaaacgtaa agagcttaaa acacactacc aggcagacat 4981 tgagcggatg tatggaagaa aaggatccta cccatacgac gtacctgatt atgcaactta 5041 tccttatgat gtacctgatt atgctagcta tccttatgat gtcccagact acgcatagtc 5101 tagataattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt 5161 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac 5221 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac 5281 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca 5341 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa 5401 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca 5461 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg 5521 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc 5581 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc 5641 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 5701 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 5761 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 5821 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 5881 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 5941 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 6001 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 6061 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 6121 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 6181 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt 6241 ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 6301 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 6361 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 6421 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 6481 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 6541 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 6601 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg 6661 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 6721 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 6781 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg 6841 aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca 6901 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca 6961 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 7021 tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc 7081 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca 7141 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag 7201 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc 7261 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta 7321 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga 7381 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact 7441 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc 7501 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 7561 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 7621 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 7681 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 7741 tgagaggcct tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat 7801 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 7861 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 7921 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 7981 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 8041 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 8101 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 8161 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 8221 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 8281 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 8341 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 8401 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 8461 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 8521 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 8581 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 8641 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 8701 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 8761 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 8821 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 8881 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 8941 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 9001 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 9061 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 9121 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 9181 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 9241 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 9301 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 9361 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 9421 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 9481 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 9541 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 9601 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 9661 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 9721 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 9781 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 9841 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 9901 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 9961 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat 10021 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 10081 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat 10141 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta 10201 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata 10261 tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt actacaaact 10321 tagtagt //