LOCUS       V044592                10264 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044592
VERSION     V044592
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..4939
                     /label="Acsl1(NM_001424792)"
                     /note="Acsl1(NM_001424792)"
                     /gene="Acsl1"
     CDS             4946..4972
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4976..5002
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5006..5032
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5052..5551
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5572..6171
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6185..6773
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6980..7613
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7742..7758)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7766..7782
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7790..7820)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7835..7856
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8144..8732)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8903..9763)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9764..9868)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9916..10050
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatggaagt ccatgaattg ttccggtatt ttcgaatgcc
     2881 agagctgatt gacattcggc agtacgtgcg cacccttcca accaacaccc tcatggggtt
     2941 tggggctttt gcagcgctca ccaccttctg gtatgccacc aggcctaagg ccctgaagcc
     3001 accatgtgac ctctccatgc agtcagtgga aatagcgggt accactgatg gtattcgaag
     3061 atcagcagtc cttgaagatg acaagctctt ggtgtactac tacgacgatg tcagaaccat
     3121 gtacgatggc ttccagaggg ggattcaggt gtcaaataat ggtccttgtt taggttctcg
     3181 gaagccaaac cagccctatg agtggatttc ctacaaagag gtggcagaac tggctgagtg
     3241 cataggctcc gggctgatcc agaaggggtt caagccttgc tccgagcagt tcatcggcct
     3301 cttctctcaa aacagacccg agtgggtgat cgtcgagcaa ggatgcttct cttactcaat
     3361 ggtggtcgtc ccgctctatg acacccttgg agctgacgcc atcacctaca tagtgaacaa
     3421 agctgaactc tctgtgattt ttgctgacaa gccagaaaaa gccaaactct tattagaagg
     3481 tgtagaaaac aagttaacac catgccttaa aatcatagtc atcatggact cctacggcag
     3541 tgatctggtg gaacgaggca agaagtgtgg ggtggaaatc atcagcctca aagctctgga
     3601 ggaccttgga agagtgaaca gagtgaagcc caagcctcca gaacccgaag atcttgcgat
     3661 aatttgtttc acaagtggaa ctacaggcaa ccccaaagga gcaatgatca ctcaccaaaa
     3721 cattataaac gactgctcag gttttataaa agcaacagag agtgcattca tcgcttccac
     3781 agatgatgtg ctgatatctt tcttgcctct cgcccatatg tttgagaccg ttgtagagtg
     3841 tgtaatgctg tgtcatggag ctaagatagg atttttccaa ggagatatca ggctgcttat
     3901 ggacgacctc aaggtgcttc agcccaccat cttccctgtg gttcccaggc tgctgaaccg
     3961 gatgttcgac agaatttttg gacaagcaaa cacttccttg aagcgatggc tgttggactt
     4021 tgcctccaaa aggaaagagg cggagcttcg cagtggcatc gtcagaaaca acagcctgtg
     4081 ggataaactc atcttccaca agatacagtc gagcctgggt gggaaagtcc ggctgatgat
     4141 cacaggagca gccccggtgt ctgccacagt gctgacgttt ctgaggacag cgctcggctg
     4201 ccagttctat gaaggctacg gacagaccga gtgcactgct ggttgctgcc tgagcttgcc
     4261 cggagactgg acggcaggcc atgttggagc ccccatgcct tgcaattatg taaagcttgt
     4321 ggatgtggaa gaaatgaatt acctggcatc caagggcgag ggtgaggtgt gtgtgaaagg
     4381 ggcaaatgtg ttcaaaggct acttgaaaga cccagcaaga acagctgaag ccctggataa
     4441 agatggctgg ttacacacgg gggacattgg aaaatggctg ccaaatggca ccttgaagat
     4501 tatcgacagg aaaaagcaca tatttaaact agcccaagga gagtacatag caccagaaaa
     4561 gattgaaaat atctacctgc ggagtgaagc cgtggcccag gtgtttgtcc acggagaaag
     4621 cttgcaggcc tttctcatag cagttgtggt acccgacgtt gagagcctac cgtcctgggc
     4681 acagaagaga ggcttacaag ggtccttcga agaactgtgc aggaacaagg atatcaataa
     4741 agctatcctg gacgacttgt tgaaacttgg gaaggaagcc ggtctgaagc catttgaaca
     4801 ggtcaaaggc attgctgtgc acccggaatt attttctatt gacaacggcc ttctgactcc
     4861 aacactgaag gcgaagaggc cagagctacg gaactatttc aggtcgcaga tagatgaact
     4921 gtacgccacc atcaagatcg gatcctaccc atacgacgta cctgattatg caacttatcc
     4981 ttatgatgta cctgattatg ctagctatcc ttatgatgtc ccagactacg catagtctag
     5041 ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     5101 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     5161 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     5221 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     5281 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     5341 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     5401 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     5461 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     5521 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag
     5581 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     5641 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     5701 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     5761 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     5821 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     5881 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     5941 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     6001 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     6061 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     6121 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg
     6181 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6241 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6301 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6361 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6421 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6481 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6541 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     6601 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6661 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6721 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     6781 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     6841 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     6901 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     6961 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     7021 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
     7081 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
     7141 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
     7201 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
     7261 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
     7321 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
     7381 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
     7441 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7501 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7561 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7621 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7681 gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat
     7741 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7801 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7861 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7921 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7981 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8041 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8101 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8161 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8221 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8281 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8341 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8401 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8461 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8521 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8581 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8641 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8701 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8761 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8821 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8881 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8941 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9001 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9061 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9121 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9181 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9241 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9301 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9361 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9421 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9481 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9541 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9601 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9661 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9721 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9781 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9841 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     9901 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9961 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10021 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    10081 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    10141 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    10201 actacaaact tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag
    10261 tagt
//
