LOCUS V029514 11526 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029514 VERSION V029514 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2827..6222 /label="ATP11C(NM_173694)" /note="ATP11C(NM_173694)" /gene="ATP11C" CDS 6229..6294 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 6314..6813 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 6834..7433 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7447..8035 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 8242..8875 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(9004..9020) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 9028..9044 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(9052..9082) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 9097..9118 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9406..9994) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(10165..11025) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(11026..11130) /gene="bla" /label="AmpR promoter" polyA_signal 11178..11312 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 gccaccatgc agatggtccc atctctccct ccagcctctg agtgtgctgg agaagagaaa 2881 cgagttggca cacgcacagt gtttgttggc aatcatccag tttcggaaac agaagcttac 2941 attgcacaaa gattttgtga taatagaata gtctcatcta agtatacact ttggaatttt 3001 ctcccaaaga atctgtttga acagtttaga agaattgcaa atttttattt tctcataatc 3061 ttccttgtac aggtcacagt agacacacca actagcccag ttaccagtgg acttccactt 3121 ttctttgtta taactgttac agccatcaag cagggatatg aggattgtct gagacacaga 3181 gctgacaatg aagtcaacaa aagcactgtt tacattattg aaaatgcaaa gcgagtgaga 3241 aaagaaagtg aaaaaatcaa ggttggtgat gtagtagaag tacaggcaga tgaaaccttt 3301 ccctgtgatc ttattcttct atcatcttgc accactgatg gaacctgtta tgtcactaca 3361 gccagtcttg atggggaatc caattgcaag acacattatg cagtacgtga taccattgca 3421 ctgtgtacag cagaatccat cgataccctc cgagcagcaa ttgaatgtga acagcctcaa 3481 cctgacctct acaaatttgt tgggcgaatc aatatctaca gtaatagtct tgaggctgtt 3541 gccaggtctt tgggacctga aaatctcttg ctgaaaggag ctacgctaaa aaataccgag 3601 aagatatatg gagttgctgt ttacactgga atggaaacca aaatggcttt gaactaccaa 3661 gggaaatctc agaaacgttc tgctgttgaa aaatctatta atgctttcct gattgtatat 3721 ttatttatct tactgaccaa agctgcagta tgcactactc taaagtatgt ttggcaaagt 3781 accccataca atgatgaacc ttggtataac caaaagactc agaaagagcg agagaccttg 3841 aaggttttaa aaatgttcac cgacttccta tcatttatgg ttctattcaa ctttatcatt 3901 cctgtctcca tgtacgtcac agtagaaatg cagaaattct tgggctcctt cttcatctca 3961 tgggataagg acttttatga tgaagaaatt aatgaaggag ccctggttaa cacatcagac 4021 cttaatgaag aacttggtca ggtggattat gtatttacag ataagactgg aacactcact 4081 gaaaacagca tggaattcat tgaatgctgc atagatggcc acaaatataa aggtgtaact 4141 caagaggttg atggattatc tcaaactgat ggaactttaa catattttga caaagtagat 4201 aagaatcgag aagagctgtt tctacgtgcc ttgtgtttat gtcatactgt agaaatcaaa 4261 acaaacgatg ctgttgatgg agctacagaa tcagctgaat taacctatat ctcctcttca 4321 ccagatgaaa tagctttggt gaaaggagct aaaaggtacg ggttcacatt tttaggaaat 4381 cgaaatggat atatgagagt agagaaccaa agaaaagaaa tagaagaata tgaacttctt 4441 cacaccttaa actttgatgc tgtccggcga cgtatgagtg taattgtgaa gactcaagaa 4501 ggagacatac ttctcttttg taaaggagca gactcggcag tttttcccag agtgcaaaat 4561 catgaaattg agttaactaa agtccatgtg gaacgtaatg caatggatgg gtatcggaca 4621 ctctgtgtag ccttcaaaga aattgctcca gatgattatg aaagaattaa cagacagctc 4681 atagaggcaa aaatggcctt acaagacaga gaagaaaaaa tggaaaaagt tttcgatgat 4741 attgagacaa acatgaattt aattggagcc actgcagttg aagacaagct acaagatcaa 4801 gctgcagaga ccattgaagc tctgcatgca gcaggcctga aagtctgggt gctcactggg 4861 gacaagatgg agacagctaa atccacatgc tatgcctgcc gccttttcca gaccaacact 4921 gagctcttag aactaaccac aaaaaccatt gaagaaagtg aaaggaaaga agatcgatta 4981 catgaattat tgatagaata tcgcaagaaa ttgctgcatg agtttcctaa aagtactaga 5041 agctttaaaa aagcatggac agaacatcag gaatatggat taatcataga tggctccaca 5101 ttgtcactca tactaaattc tagtcaagac tctagttcaa acaattacaa aagcattttc 5161 ctacaaatat gtatgaagtg tactgcagtg ctctgctgtc ggatggcacc attacagaaa 5221 gcccagattg tcagaatggt gaagaattta aaaggcagcc caataactct gtcgataggt 5281 gatggtgcca atgatgttag tatgatcttg gaatcccatg tgggaatagg tattaaaggc 5341 aaagaaggtc gccaagcagc taggaatagc gattattctg ttccaaagtt taaacactta 5401 aagaaactgc tgttggctca tggacatcta tattatgtga gaatagcaca ccttgtacag 5461 tacttcttct ataagaacct ttgtttcatt ttgccacagt ttttgtacca gttcttctgt 5521 ggattctcac aacagccact gtatgatgct gcttacctta caatgtacaa tatctgcttc 5581 acatccttgc ccatcctggc ctatagtcta ctggaacagc acatcaacat tgacactctg 5641 acctcagatc cccgattgta tatgaaaatt tctggcaatg ccatgctaca gttgggcccc 5701 ttcttatatt ggacatttct ggctgccttt gaagggacag tgttcttctt tgggacttac 5761 tttctttttc agactgcatc cctagaagaa aatggaaagg tatacggaaa ctggactttt 5821 ggaaccattg tttttacagt cttagtattc actgtaaccc tgaagcttgc cttggatacc 5881 cgattctgga cgtggataaa tcactttgtg atttggggtt ctttagcctt ctatgtattt 5941 ttctcattct tctggggagg aattatttgg ccttttctca agcaacagag aatgtatttt 6001 gtatttgccc aaatgctgtc ttctgtatcc acatggttgg ctataattct tctaatattt 6061 atcagcctgt tccctgagat tcttctgata gtattaaaga atgtaagaag aagaagtgcc 6121 aggagaaatc tgagctgtag aagggcatct gactcattat ccgccagacc ttcagtcaga 6181 cctcttcttt tacgaacatt ctcagacgaa tctaatgtat tgggatccga ctacaaagac 6241 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagtct 6301 agataattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta 6361 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca 6421 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact 6481 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa 6541 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag 6601 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag 6661 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc 6721 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg 6781 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg 6841 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc 6901 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca 6961 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 7021 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 7081 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 7141 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 7201 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 7261 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 7321 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 7381 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc 7441 tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 7501 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 7561 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 7621 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 7681 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 7741 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 7801 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg 7861 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 7921 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 7981 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga 8041 attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag 8101 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag 8161 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact 8221 ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc 8281 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag 8341 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc 8401 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc 8461 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag 8521 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag 8581 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg 8641 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct 8701 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 8761 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 8821 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 8881 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 8941 gagaggcctt gacattgcta gcgttttacc gtcgacctct agctagagct tggcgtaatc 9001 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 9061 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 9121 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 9181 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 9241 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 9301 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 9361 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 9421 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 9481 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 9541 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 9601 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 9661 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 9721 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 9781 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 9841 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 9901 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 9961 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 10021 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 10081 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 10141 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 10201 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 10261 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 10321 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 10381 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 10441 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 10501 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 10561 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 10621 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 10681 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 10741 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 10801 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 10861 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 10921 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 10981 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 11041 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 11101 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 11161 cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata 11221 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 11281 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc 11341 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac 11401 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat 11461 gtactacaaa cttagtagtt tttaaagaaa ttgtatttgt taaatatgta ctacaaactt 11521 agtagt //