LOCUS       V019909                 9251 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019909
VERSION     V019909
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..4007
                     /label="ALKBH5(NM_017758)"
                     /note="ALKBH5(NM_017758)"
                     /gene="ALKBH5"
     promoter        4039..4538
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4559..5158
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5172..5760
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5967..6600
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(6729..6745)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6753..6769
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6777..6807)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6822..6843
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7131..7719)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7890..8750)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8751..8855)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    8903..9037
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatggc ggccgccagc ggctacacgg acctgcgtga gaagctcaag tccatgacgt
     2881 cccgggacaa ctataaggcg ggcagccggg aggccgccgc cgctgccgca gccgccgtag
     2941 ccgccgcagc cgcagccgcc gctgccgccg aaccttaccc tgtgtccggg gccaagcgca
     3001 agtatcagga ggactcggac cccgagcgca gcgactatga ggagcagcag ctgcagaagg
     3061 aggaggaggc gcgcaaggtg aagagcggca tccgccagat gcgcctcttc agccaggacg
     3121 agtgcgccaa gatcgaggcc cgcattgacg aggtggtgtc ccgcgctgag aagggcctgt
     3181 acaacgagca cacggtggac cgggccccac tgcgcaacaa gtacttcttc ggcgaaggct
     3241 acacttacgg cgcccagctg cagaagcgcg ggcccggcca ggagcgcctc tacccgccgg
     3301 gcgacgtgga cgagatcccc gagtgggtgc accagctggt gatccaaaag ctggtggagc
     3361 accgcgtcat ccccgagggc ttcgtcaaca gcgccgtcat caacgactac cagcccggcg
     3421 gctgcatcgt gtctcacgtg gaccccatcc acatcttcga gcgccccatc gtgtccgtgt
     3481 ccttctttag cgactctgcg ctgtgcttcg gctgcaagtt ccagttcaag cctattcggg
     3541 tgtcggaacc agtgctttcc ctgccggtgc gcaggggaag cgtgactgtg ctcagtggat
     3601 atgctgctga tgaaatcact cactgcatac ggcctcagga catcaaggag cgccgagcag
     3661 tcatcatcct caggaagaca agattagatg caccccggtt ggaaacaaag tccctgagca
     3721 gctccgtgtt accacccagc tatgcttcag atcgcctgtc aggaaacaac agggaccctg
     3781 ctctgaaacc caagcggtcc caccgcaagg cagaccctga tgctgcccac aggccacgga
     3841 tcctggagat ggacaaggaa gagaaccggc gctcggtgct gctgcccaca caccggcgga
     3901 ggggtagctt cagctctgag aactactggc gcaagtcata cgagtcctca gaggactgct
     3961 ctgaggcagc aggcagccct gcccgaaagg tgaagatgcg gcggcactga ggatcccgcg
     4021 actctagata attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     4081 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     4141 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     4201 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     4261 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     4321 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     4381 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     4441 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     4501 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     4561 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     4621 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     4681 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     4741 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     4801 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     4861 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     4921 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     4981 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     5041 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     5101 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc
     5161 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5221 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5281 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5341 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5401 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5461 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     5521 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     5581 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     5641 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5701 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5761 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     5821 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     5881 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     5941 ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga
     6001 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     6061 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     6121 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     6181 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     6241 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     6301 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     6361 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     6421 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6481 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6541 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6601 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     6661 agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg
     6721 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6781 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6841 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6901 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6961 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7021 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7081 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7141 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7201 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7261 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7321 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7381 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7441 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7501 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7561 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7621 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7681 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7741 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7801 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7861 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7921 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7981 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8041 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8101 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8161 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8221 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8281 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8341 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8401 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8461 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8521 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8581 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8641 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8701 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8761 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8821 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8881 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
     8941 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9001 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
     9061 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
     9121 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
     9181 aatatgtact acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca
     9241 aacttagtag t
//
