LOCUS       V013442                 8777 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013442
VERSION     V013442
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2201..2699
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     misc_feature    2787..3373
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             3375..4067
                     /label="TurboRFP"
                     /note="red fluorescent protein from Entacmaea quadricolor"
     CDS             4086..4682
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    4699..5287
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5494..6127
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6255..6271)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6279..6295)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6303..6333)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6348..6369)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6657..7245)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7419..8276)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8277..8381)
                     /label="AmpR promoter"
     polyA_signal    8429..8563
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg gggtagggga ggcgcttttc
     2221 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     2281 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     2341 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     2401 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     2461 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     2521 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     2581 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     2641 acgcttcgaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     2701 aaagggcaga ggaagtcttc taacttgagg tgacgtggag gagaatgaat tcctcgagac
     2761 tagttctaga gcggccgcgg atcccgcccc tctccctccc ccccccctaa cgttactggc
     2821 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     2881 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     2941 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     3001 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     3061 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     3121 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     3181 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     3241 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     3301 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     3361 gcttgccaca acccatgagc gagctgatca aggagaacat gcacatgaag ctgtacatgg
     3421 agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg
     3481 agggcaccca gaccatgaag atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
     3541 acatcctggc taccagcttc atgtacggca gcaaagcctt catcaaccac acccagggca
     3601 tccccgactt ctttaagcag tccttccctg agggcttcac atgggagaga atcaccacat
     3661 acgaagacgg gggcgtgctg accgctaccc aggacaccag cttccagaac ggctgcatca
     3721 tctacaacgt caagatcaac ggggtgaact tcccatccaa cggccctgtg atgcagaaga
     3781 aaacacgcgg ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc ggcctgagag
     3841 gccacagcca gatggccctg aagctcgtgg gcgggggcta cctgcactgc tccttcaaga
     3901 ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcttc cacttcgtgg
     3961 accacagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgaga
     4021 tggctgtggc caagtactgc gacctcccta gcaaactggg gcacagaaca aggagacgac
     4081 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     4141 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     4201 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     4261 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     4321 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     4381 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     4441 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     4501 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     4561 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     4621 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     4681 cctagacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4741 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4801 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4861 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4921 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4981 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5041 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     5101 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5161 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5221 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5281 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta
     5341 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     5401 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     5461 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa
     5521 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     5581 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     5641 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     5701 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     5761 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     5821 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     5881 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     5941 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6001 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6061 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6121 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     6181 atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc
     6241 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6301 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6361 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6421 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6481 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6541 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6601 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6661 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6721 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6781 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6841 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6901 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6961 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7021 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7081 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7141 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7201 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7261 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7321 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7381 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7441 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7501 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7561 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7621 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7681 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7741 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7801 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7861 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7921 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7981 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8041 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8101 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8161 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8221 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8281 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8341 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8401 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa
     8461 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8521 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
     8581 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
     8641 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
     8701 ttgttaaata tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt
     8761 actacaaact tagtagt
//
