LOCUS       V015218                10419 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015218
VERSION     V015218
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10419)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             5152..5175
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     promoter        5207..5706
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5727..6323
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6340..6928
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7135..7768
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7945..7975)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7990..8011)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8299..8887)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9061..9918)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9919..10023)
                     /label="AmpR promoter"
     polyA_signal    10071..10205
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatgg caaaacgaaa gcgtaagcat ggctctctct ggctgcctga aggtttcact
     2881 cttcgccgag atgcaagcaa tcacaagcgt cgtgagccag tgggacagga tgctgtgggg
     2941 ctgaaaaatc tctcagtgca agtctcagaa gctaacctaa ttggtactgg aacaagtgaa
     3001 cactgggtcg atgatgaagg gccccagcca aagaaagtaa aggctgaaga tgaggcctta
     3061 ctctcagaag aagatgaccc cattgatcga cggccatgga cacagcagca ccttgaagct
     3121 gcagacatcc gtaggacacc atcgctggct ctcacccctc ctcaggcaga gcaggaggtg
     3181 gatgtgttag atgtgaatgt ccgtggccca gatggctgca ccccattgat gttggcttct
     3241 ctccgaggag gcagctcaga tttgagtgat gaagatgaag atgcagagga ctcttctgct
     3301 aacatcatca cagacttggt ctaccagggt gccagcctcc aggcccagac agaccggact
     3361 ggtgagatgg ccctgcacct tgcagcccgc tactcacggg ctgatgctgc caagcgtctc
     3421 ctggatgcag gtgcagatgc caatgcccag gacaacatgg gccgctgtcc actccatgct
     3481 gcagtggcag ctgatgccca aggtgtcttc cagattctga ttcgcaaccg agtaactgat
     3541 ctagatgcca ggatgaatga tggtactaca cccctgatcc tggctgcccg cctggctgtg
     3601 gagggaatgg tggcagaact gatcaactgc caagcggatg tgaatgcagt ggatgaccat
     3661 ggaaaatctg ctcttcactg ggcagctgct gtcaataatg tggaggcaac tcttttgttg
     3721 ttgaaaaatg gggccaaccg agacatgcag gacaacaagg aagagacacc tctgtttctt
     3781 gctgcccggg aggggagcta tgaagcagcc aagatcctgt tagaccattt tgccaatcga
     3841 gacatcacag accatatgga tcgtcttccc cgggatgtgg ctcgggatcg catgcaccat
     3901 gacattgtgc gccttctgga tgaatacaat gtgaccccaa gccctccagg caccgtgttg
     3961 acttctgctc tctcacctgt catctgtggg cccaacagat ctttcctcag cctgaagcac
     4021 accccaatgg gcaagaagtc tagacggccc agtgccaaga gtaccatgcc tactagcctc
     4081 cctaaccttg ccaaggaggc aaaggatgcc aagggtagta ggaggaagaa gtctctgagt
     4141 gagaaggtcc aactgtctga gagttcagta actttatccc ctgttgattc cctagaatct
     4201 cctcacacgt atgtttccga caccacatcc tctccaatga ttacatcccc tgggatctta
     4261 caggcctcac ccaaccctat gttggccact gccgcccctc ctgccccagt ccatgcccag
     4321 catgcactat ctttttctaa ccttcatgaa atgcagcctt tggcacatgg ggccagcact
     4381 gtgcttccct cagtgagcca gttgctatcc caccaccaca ttgtgtctcc aggcagtggc
     4441 agtgctggaa gcttgagtag gctccatcca gtcccagtcc cagcagattg gatgaaccgc
     4501 atggaggtga atgagaccca gtacaatgag atgtttggta tggtcctggc tccagctgag
     4561 ggcacccatc ctggcatagc tccccagagc aggccacctg aagggaagca cataaccacc
     4621 cctcgggagc ccttgccccc cattgtgact ttccagctca tccctaaagg cagtattgcc
     4681 caaccagcgg gggctcccca gcctcagtcc acctgccctc cagctgttgc gggccccctg
     4741 cccaccatgt accagattcc agaaatggcc cgtttgccca gtgtggcttt ccccactgcc
     4801 atgatgcccc agcaggacgg gcaggtagct cagaccattc tcccagccta tcatcctttc
     4861 ccagcctctg tgggcaagta ccccacaccc ccttcacagc acagttatgc ttcctcaaat
     4921 gctgctgagc gaacacccag tcacagtggt cacctccagg gtgagcatcc ctacctgaca
     4981 ccatccccag agtctcctga ccagtggtca agttcatcac cccactctgc ttctgactgg
     5041 tcagatgtga ccaccagccc tacccctggg ggtgctggag gaggtcagcg gggacctggg
     5101 acacacatgt ctgagccacc acacaacaac atgcaggttt atgcggaatt cgattacaag
     5161 gatgacgacg ataagtgagg atcccgcgac tctagataat tctaccgggt aggggaggcg
     5221 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     5281 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     5341 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     5401 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     5461 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     5521 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     5581 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     5641 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     5701 ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     5761 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     5821 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     5881 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     5941 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     6001 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     6061 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     6121 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     6181 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     6241 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     6301 gcatgacccg caagcccggt gcctgaccgc gtctggaaca atcaacctct ggattacaaa
     6361 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6421 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6481 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6541 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6601 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6661 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6721 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     6781 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6841 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6901 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
     6961 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     7021 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     7081 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
     7141 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     7201 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     7261 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     7321 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     7381 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     7441 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     7501 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     7561 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     7621 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7681 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7741 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7801 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgtttt
     7861 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7921 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7981 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8041 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8101 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8161 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8221 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8281 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8341 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8401 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8461 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8521 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8581 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8641 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8701 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8761 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8821 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8881 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8941 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9001 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9061 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9121 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9181 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9241 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9301 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9361 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9421 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9481 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9541 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9601 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9661 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9721 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9781 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9841 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9901 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9961 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10021 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta
    10081 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    10141 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    10201 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
    10261 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
    10321 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gtttttaaag
    10381 aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
