LOCUS       .                       9539 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2346..2377
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             2439..4088
                     /label="luciferase"
                     /note="firefly luciferase"
     polyA_signal    complement(4132..4253)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        4327..4826
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4847..5443
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    5460..6048
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6255..6888
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7017..7033)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7041..7057)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7065..7095)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7110..7131)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7419..8007)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8181..9038)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(9039..9143)
                     /label="AmpR promoter"
     polyA_signal    9191..9325
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataggtac cgagctctta cgcgagatca aagggggtaa
     2221 gatcaaaggg ggtaagatca aaggggcgcg agatcaaagg gggtaagatc aaagggggta
     2281 agatcaaagg gggtaagatc aaaggggcgc gcccgcgtgc tagcccgggc tcgagatcta
     2341 gactctagag ggtatataat ggaagctcga attccagctt ggcattccgg tactgttggt
     2401 aaaaagcttg gcattccggt actgttggta aagccaccat ggaagacgcc aaaaacataa
     2461 agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata
     2521 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
     2581 aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga
     2641 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
     2701 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
     2761 tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg
     2821 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa
     2881 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg
     2941 tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata
     3001 gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg
     3061 tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg
     3121 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
     3181 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
     3241 ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg
     3301 tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta
     3361 atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg
     3421 ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag
     3481 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat
     3541 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag
     3601 gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga
     3661 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
     3721 aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc
     3781 aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag
     3841 gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
     3901 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
     3961 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
     4021 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga
     4081 tcgccgtgta attctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca
     4141 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     4201 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     4261 acaattgcat tcattttatg tttcaggttc agggggaggg atcccgcgac tctagataat
     4321 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     4381 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     4441 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     4501 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     4561 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     4621 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     4681 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     4741 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     4801 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa
     4861 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     4921 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     4981 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     5041 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     5101 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     5161 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     5221 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     5281 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     5341 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     5401 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaccgc gtctggaaca
     5461 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5521 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5581 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5641 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5701 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5761 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5821 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     5881 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5941 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6001 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt
     6061 ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     6121 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     6181 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6241 agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     6301 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     6361 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     6421 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     6481 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     6541 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     6601 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     6661 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     6721 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6781 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6841 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     6901 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     6961 cttgacattg ctagcgtttt accgtcgacc tctagctaga gcttggcgta atcatggtca
     7021 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7081 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7141 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7201 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7261 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7321 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7381 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7441 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7501 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7561 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7621 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7681 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7741 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7801 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7861 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7921 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7981 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8041 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8101 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8161 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8221 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8281 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8341 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8401 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8461 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8521 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8581 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8641 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8701 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8761 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8821 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8881 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8941 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9001 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9061 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9121 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     9181 tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9241 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9301 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
     9361 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
     9421 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
     9481 aaacttagta gtttttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
