LOCUS       V013061                 9860 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013061
VERSION     V013061
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2815..4464
                     /label="luciferase"
                     /note="firefly luciferase"
     misc_feature    4499..5078
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5073..5765
                     /label="ZsGreen1"
                     /note="Zoanthus green fluorescent protein"
     misc_feature    5782..6370
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6577..7210
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7338..7354)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7362..7378)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7386..7416)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7431..7452)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7740..8328)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8502..9359)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9360..9464)
                     /label="AmpR promoter"
     polyA_signal    9512..9646
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggaa
     2821 gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct
     2881 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt
     2941 acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg
     3001 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt
     3061 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt
     3121 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag
     3181 cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag
     3241 ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag
     3301 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg
     3361 ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact
     3421 ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc
     3481 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca
     3541 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc
     3601 gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt
     3661 caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac
     3721 aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa
     3781 gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc
     3841 actgagacta catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc
     3901 ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg
     3961 ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta
     4021 aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac
     4081 atagcttact gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt
     4141 aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc
     4201 aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc
     4261 gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc
     4321 gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta
     4381 ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc
     4441 aagaagggcg gaaagatcgc cgtgctcgag actagttcta gagcggccgc ggatcccgcc
     4501 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     4561 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     4621 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     4681 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     4741 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     4801 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     4861 ttgtgagttg gatagttgtg gaaagagtca aatggctcac ctcaagcgta ttcaacaagg
     4921 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     4981 catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga
     5041 cgtggttttc ctttgaaaaa cacgatgata atatggccca gtccaagcac ggcctgacca
     5101 aggagatgac catgaagtac cgcatggagg gctgcgtgga cggccacaag ttcgtgatca
     5161 ccggcgaggg catcggctac cccttcaagg gcaagcaggc catcaacctg tgcgtggtgg
     5221 agggcggccc cttgcccttc gccgaggaca tcttgtccgc cgccttcatg tacggcaacc
     5281 gcgtgttcac cgagtacccc caggacatcg tcgactactt caagaactcc tgccccgccg
     5341 gctacacctg ggaccgctcc ttcctgttcg aggacggcgc cgtgtgcatc tgcaacgccg
     5401 acatcaccgt gagcgtggag gagaactgca tgtaccacga gtccaagttc tacggcgtga
     5461 acttccccgc cgacggcccc gtgatgaaga agatgaccga caactgggag ccctcctgcg
     5521 agaagatcat ccccgtgccc aagcagggca tcttgaaggg cgacgtgagc atgtacctgc
     5581 tgctgaagga cggtggccgc ttgcgctgcc agttcgacac cgtgtacaag gccaagtccg
     5641 tgccccgcaa gatgcccgac tggcacttca tccagcacaa gctgacccgc gaggaccgca
     5701 gcgacgccaa gaaccagaag tggcacctga ccgagcacgc catcgcctcc ggctccgcct
     5761 tgccctgaac gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
     5821 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5881 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5941 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6001 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6061 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6121 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     6181 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6241 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6301 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6361 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa
     6421 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     6481 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     6541 tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac
     6601 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     6661 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     6721 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     6781 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     6841 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     6901 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     6961 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     7021 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7081 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7141 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7201 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7261 tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac ctctagctag
     7321 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7381 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7441 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7501 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7561 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7621 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7681 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7741 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7801 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7861 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7921 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7981 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8041 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8101 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8161 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8221 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8281 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8341 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8401 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8461 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8521 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8581 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8641 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8701 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8761 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8821 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8881 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8941 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9001 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9061 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9121 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9181 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9241 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9301 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9361 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9421 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9481 gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta
     9541 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9601 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     9661 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
     9721 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
     9781 tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata
     9841 tgtactacaa acttagtagt
//
