LOCUS       V013080                10407 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013080
VERSION     V013080
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2815..4464
                     /label="luciferase"
                     /note="firefly luciferase"
     CDS             4471..5034
                     /label="K-Ras (G12V)"
                     /note="human oncoprotein generated by the G12V mutation in
                     the small GTPase K-Ras"
     misc_feature    5046..5625
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5620..6312
                     /label="ZsGreen1"
                     /note="Zoanthus green fluorescent protein"
     misc_feature    6329..6917
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7124..7757
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7933..7963)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7978..7999)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8287..8875)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9049..9906)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9907..10011)
                     /label="AmpR promoter"
     polyA_signal    10059..10193
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggaa
     2821 gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct
     2881 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt
     2941 acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg
     3001 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt
     3061 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt
     3121 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag
     3181 cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag
     3241 ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag
     3301 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg
     3361 ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact
     3421 ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc
     3481 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca
     3541 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc
     3601 gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt
     3661 caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac
     3721 aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa
     3781 gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc
     3841 actgagacta catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc
     3901 ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg
     3961 ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta
     4021 aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac
     4081 atagcttact gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt
     4141 aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc
     4201 aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc
     4261 gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc
     4321 gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta
     4381 ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc
     4441 aagaagggcg gaaagatcgc cgtgctcgag atgactgaat ataaacttgt ggtagttgga
     4501 gctgttggcg taggcaagag tgccttgacg atacagctaa ttcagaatca ttttgtggac
     4561 gaatatgatc caacaataga ggattcctac aggaagcaag tagtaattga tggagaaacc
     4621 tgtctcttgg atattctcga cacagcaggt caagaggagt acagtgcaat gagggaccag
     4681 tacatgagga ctggggaggg ctttctttgt gtatttgcca taaataatac taaatcattt
     4741 gaagatattc accattatag agaacaaatt aaaagagtta aggactctga agatgtacct
     4801 atggtcctag taggaaataa atgtgatttg ccttctagaa cagtagacac aaaacaggct
     4861 caggacttag caagaagtta tggaattcct tttattgaaa catcagcaaa gacaagacag
     4921 ggtgttgatg atgccttcta tacattagtt cgagaaattc gaaaacataa agaaaagatg
     4981 agcaaagatg gtaaaaagaa gaaaaagaag tcaaagacaa agtgtgtaat tatgtaagga
     5041 tcccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     5101 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     5161 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     5221 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     5281 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     5341 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     5401 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctcacctc aagcgtattc
     5461 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     5521 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc
     5581 acggggacgt ggttttcctt tgaaaaacac gatgataata tggcccagtc caagcacggc
     5641 ctgaccaagg agatgaccat gaagtaccgc atggagggct gcgtggacgg ccacaagttc
     5701 gtgatcaccg gcgagggcat cggctacccc ttcaagggca agcaggccat caacctgtgc
     5761 gtggtggagg gcggcccctt gcccttcgcc gaggacatct tgtccgccgc cttcatgtac
     5821 ggcaaccgcg tgttcaccga gtacccccag gacatcgtcg actacttcaa gaactcctgc
     5881 cccgccggct acacctggga ccgctccttc ctgttcgagg acggcgccgt gtgcatctgc
     5941 aacgccgaca tcaccgtgag cgtggaggag aactgcatgt accacgagtc caagttctac
     6001 ggcgtgaact tccccgccga cggccccgtg atgaagaaga tgaccgacaa ctgggagccc
     6061 tcctgcgaga agatcatccc cgtgcccaag cagggcatct tgaagggcga cgtgagcatg
     6121 tacctgctgc tgaaggacgg tggccgcttg cgctgccagt tcgacaccgt gtacaaggcc
     6181 aagtccgtgc cccgcaagat gcccgactgg cacttcatcc agcacaagct gacccgcgag
     6241 gaccgcagcg acgccaagaa ccagaagtgg cacctgaccg agcacgccat cgcctccggc
     6301 tccgccttgc cctgaacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag
     6361 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6421 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6481 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6541 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6601 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6661 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6721 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6781 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6841 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6901 ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca
     6961 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     7021 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     7081 gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac
     7141 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     7201 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
     7261 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
     7321 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
     7381 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
     7441 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
     7501 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
     7561 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7621 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7681 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7741 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     7801 aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc
     7861 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7921 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7981 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8041 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8101 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8161 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8221 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8281 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8341 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8401 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8461 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8521 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8581 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8641 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8701 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8761 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8821 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8881 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8941 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9001 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9061 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9121 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9181 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9241 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9301 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9361 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9421 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9481 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9541 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9601 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9661 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9721 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9781 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9841 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9901 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9961 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10021 ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata
    10081 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    10141 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    10201 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    10261 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    10321 gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa attgtatttg
    10381 ttaaatatgt actacaaact tagtagt
//
