LOCUS       V032166                10590 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032166
VERSION     V032166
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..5055
                     /label="QSOX1(NM_002826)"
                     /note="QSOX1(NM_002826)"
                     /gene="QSOX1"
     CDS             5062..5127
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    5164..5750
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             5751..6545
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     misc_feature    6559..7147
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7354..7987
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8115..8131)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8139..8155
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8163..8193)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8208..8229
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8517..9105)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9276..10136)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10137..10241)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10289..10423
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgagg
     2821 aggtgcaaca gcggctccgg gccgccgccg tcgctgctgc tgctgctgct gtggctgctc
     2881 gcggttcccg gcgctaacgc ggccccgcgg tcggcgctct attcgccttc cgacccgctg
     2941 acgctgctgc aggcggacac ggtgcgcggc gcggtgctgg gctcccgcag cgcctgggcc
     3001 gtggagttct tcgcctcctg gtgcggccac tgcatcgcct tcgccccgac gtggaaggcg
     3061 ctggccgaag acgtcaaagc ctggaggccg gccctgtatc tcgccgccct ggactgtgct
     3121 gaggagacca acagtgcagt ctgcagagac ttcaacatcc ctggcttccc gactgtgagg
     3181 ttcttcaagg cctttaccaa gaacggctcg ggagcagtat ttccagtggc tggtgctgac
     3241 gtgcagacac tgcgggagag gctcattgac gccctggagt cccatcatga cacgtggccc
     3301 ccagcctgtc ccccactgga gcctgccaag ctggaggaga ttgatggatt ctttgcgaga
     3361 aataacgaag agtacctggc tctgatcttt gaaaagggag gctcctacct gggtagagag
     3421 gtggctctgg acctgtccca gcacaaaggc gtggcggtgc gcagggtgct gaacacagag
     3481 gccaatgtgg tgagaaagtt tggtgtcacc gacttcccct cttgctacct gctgttccgg
     3541 aatggctctg tctcccgagt ccccgtgctc atggaatcca ggtccttcta taccgcttac
     3601 ctgcagagac tctctgggct caccagggag gctgcccaga ccacagttgc accaaccact
     3661 gctaacaaga tagctcccac tgtttggaaa ttggcagatc gctccaagat ctacatggct
     3721 gacctggaat ctgcactgca ctacatcctg cggatagaag tgggcaggtt cccggtcctg
     3781 gaagggcagc gcctggtggc cctgaaaaag tttgtggcag tgctggccaa gtatttccct
     3841 ggccggccct tagtccagaa cttcctgcac tccgtgaatg aatggctcaa gaggcagaag
     3901 agaaataaaa ttccctacag tttctttaaa actgccctgg acgacaggaa agagggtgcc
     3961 gttcttgcca agaaggtgaa ctggattggc tgccagggga gtgagccgca tttccggggc
     4021 tttccctgct ccctgtgggt cctcttccac ttcttgactg tgcaggcagc tcggcaaaat
     4081 gtagaccact cacaggaagc agccaaggcc aaggaggtcc tcccagccat ccgaggctac
     4141 gtgcactact tcttcggctg ccgagactgc gctagccact tcgagcagat ggctgctgcc
     4201 tccatgcacc gggtggggag tcccaacgcc gctgtcctct ggctctggtc tagccacaac
     4261 agggtcaatg ctcgccttgc aggtgccccc agcgaggacc cccagttccc caaggtgcag
     4321 tggccacccc gtgaactttg ttctgcctgc cacaatgaac gcctggatgt gcccgtgtgg
     4381 gacgtggaag ccaccctcaa cttcctcaag gcccacttct ccccaagcaa catcatcctg
     4441 gacttccctg cagctgggtc agctgcccgg agggatgtgc agaatgtggc agccgcccca
     4501 gagctggcga tgggagccct ggagctggaa agccggaatt caactctgga ccctgggaag
     4561 cctgagatga tgaagtcccc cacaaacacc accccacatg tgccggctga gggacctgag
     4621 gcaagtcgac ccccgaagct gcaccctggc ctcagagctg caccaggcca ggagcctcct
     4681 gagcacatgg cagagcttca gaggaatgag caggagcagc cgcttgggca gtggcacttg
     4741 agcaagcgag acacaggggc tgcattgctg gctgagtcca gggctgagaa gaaccgcctc
     4801 tggggccctt tggaggtcag gcgcgtgggc cgcagctcca agcagctggt cgacatccct
     4861 gagggccagc tggaggcccg agctggacgg ggccgaggcc agtggctgca ggtgctggga
     4921 gggggcttct cttacctgga catcagcctc tgtgtggggc tctattccct gtccttcatg
     4981 ggcctgctgg ccatgtacac ctacttccag gccaagataa gggccctgaa gggccatgct
     5041 ggccaccctg cagccctcga ggactacaaa gaccatgacg gtgattataa agatcatgac
     5101 atcgactaca aggatgacga tgacaagtag ctcgagacta gttctagagc ggccgcggat
     5161 ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     5221 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     5281 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     5341 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     5401 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     5461 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     5521 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     5581 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     5641 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     5701 cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc atggctgaac
     5761 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     5821 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     5881 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     5941 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     6001 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     6061 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     6121 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     6181 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     6241 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     6301 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     6361 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     6421 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     6481 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     6541 tctgaacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6601 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6661 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6721 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6781 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6841 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6901 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     6961 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7021 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7081 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7141 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta
     7201 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     7261 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     7321 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa
     7381 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     7441 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     7501 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     7561 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     7621 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     7681 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     7741 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     7801 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7861 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7921 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7981 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     8041 atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc
     8101 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8161 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8221 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8281 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8341 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8401 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8461 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8521 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8581 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8641 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8701 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8761 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8821 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8881 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8941 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9001 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9061 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9121 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9181 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9241 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9301 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9361 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9421 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9481 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9541 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9601 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9661 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9721 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9781 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9841 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9901 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9961 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10021 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10081 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10141 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10201 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10261 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa
    10321 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    10381 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    10441 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
    10501 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
    10561 ttgttaaata tgtactacaa acttagtagt
//
