LOCUS V020907 8872 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V020907 VERSION V020907 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2821..3393 /codon_start=1 /product="human Rho family small GTPase Cdc42" /transl_table=1 /translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVM IGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK GLKNVFDEAILAALEPPEPKKSRRCVLL" /label="Cdc42" CDS 3409..3435 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 3446..4032 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES2" CDS 4033..4827 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" misc_feature 4841..5429 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5636..6269 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6397..6413) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6421..6437 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6445..6475) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6490..6511 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6799..7387) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7558..8418) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8419..8523) /gene="bla" /label="AmpR promoter" polyA_signal 8571..8705 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgaggccacc 2821 atgcagacaa ttaagtgtgt tgttgtgggc gatggtgctg ttggtaaaac atgtctcctg 2881 atatcctaca caacaaacaa atttccatcg gaatatgtac cgactgtttt tgacaactat 2941 gcagtcacag ttatgattgg tggagaacca tatactcttg gactttttga tactgcaggg 3001 caagaggatt atgacagatt acgaccgctg agttatccac aaacagatgt atttctagtc 3061 tgtttttcag tggtctctcc atcttcattt gaaaacgtga aagaaaagtg ggtgcctgag 3121 ataactcacc actgtccaaa gactcctttc ttgcttgttg ggactcaaat tgatctcaga 3181 gatgacccct ctactattga gaaacttgcc aagaacaaac agaagcctat cactccagag 3241 actgctgaaa agctggcccg tgacctgaag gctgtcaagt atgtggagtg ttctgcactt 3301 acacagaaag gcctaaagaa tgtatttgac gaagcaatat tggctgccct ggagcctcca 3361 gaaccgaaga agagccgcag gtgtgtgctg ctaggcggtg gaggaagtta tccttatgac 3421 gtgcctgact atgcctgagg atccgcccct ctccctcccc cccccctaac gttactggcc 3481 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc 3541 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta 3601 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag 3661 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga 3721 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg 3781 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat 3841 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta 3901 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa 3961 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag 4021 cttgccacaa ccatggctga acaagatgga ttgcacgcag gttctccggc cgcttgggtg 4081 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg 4141 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc 4201 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct 4261 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa 4321 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg 4381 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa 4441 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat 4501 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg 4561 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc 4621 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac 4681 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg 4741 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 4801 tatcgccttc ttgacgagtt cttctgaacg cgtctggaac aatcaacctc tggattacaa 4861 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 4921 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 4981 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 5041 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 5101 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 5161 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 5221 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat 5281 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 5341 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 5401 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa 5461 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta 5521 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca 5581 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa 5641 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa 5701 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc 5761 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa 5821 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga 5881 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg 5941 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg 6001 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc 6061 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg 6121 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 6181 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 6241 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 6301 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt 6361 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 6421 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 6481 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 6541 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 6601 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 6661 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 6721 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 6781 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 6841 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 6901 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 6961 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 7021 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 7081 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 7141 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 7201 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 7261 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 7321 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 7381 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 7441 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 7501 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 7561 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 7621 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 7681 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 7741 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 7801 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 7861 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 7921 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 7981 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 8041 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 8101 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 8161 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 8221 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 8281 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 8341 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 8401 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 8461 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 8521 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta 8581 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 8641 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 8701 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta 8761 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat 8821 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt //