LOCUS       V014033                11080 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014033
VERSION     V014033
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11080)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2826..4448
                     /gene="WLS"
                     /label="Protein wntless homolog"
                     /note="Protein wntless homolog from Homo sapiens.
                     Accession#: Q5T9L3"
     CDS             4449..4475
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             4479..4505
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             4509..4535
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    4544..5130
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5132..5848
                     /label="EGFP"
                     /note="enhanced GFP"
     promoter        5868..6367
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             6388..6984
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    7001..7589
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7796..8429
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8606..8636)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8651..8672)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8960..9548)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9722..10579)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10580..10684)
                     /label="AmpR promoter"
     polyA_signal    10732..10866
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatggc tggggcaatt atagaaaaca tgagcaccaa gaagctgtgc attgttggtg
     2881 ggattctgct cgtgttccaa atcatcgcct ttctggtggg aggcttgatt gctccagggc
     2941 ccacaacggc agtgtcctac atgtcggtga aatgtgtgga tgcccgtaag aaccatcaca
     3001 agacaaaatg gttcgtgcct tggggaccca atcattgtga caagatccga gacattgaag
     3061 aggcaattcc aagggaaatt gaagccaatg acatcgtgtt ttctgttcac attcccctcc
     3121 cccacatgga gatgagtcct tggttccaat tcatgctgtt tatcctgcag ctggacattg
     3181 ccttcaagct aaacaaccaa atcagagaaa atgcagaagt ctccatggac gtttccctgg
     3241 cttaccgtga tgacgcgttt gctgagtgga ctgaaatggc ccatgaaaga gtaccacgga
     3301 aactcaaatg caccttcaca tctcccaaga ctccagagca tgagggccgt tactatgaat
     3361 gtgatgtcct tcctttcatg gaaattgggt ctgtggccca taagttttac cttttaaaca
     3421 tccggctgcc tgtgaatgag aagaagaaaa tcaatgtggg aattggggag ataaaggata
     3481 tccggttggt ggggatccac caaaatggag gcttcaccaa ggtgtggttt gccatgaaga
     3541 ccttccttac gcccagcatc ttcatcatta tggtgtggta ttggaggagg atcaccatga
     3601 tgtcccgacc cccagtgctt ctggaaaaag tcatctttgc ccttgggatt tccatgacct
     3661 ttatcaatat cccagtggaa tggttttcca tcgggtttga ctggacctgg atgctgctgt
     3721 ttggtgacat ccgacagggc atcttctatg cgatgcttct gtccttctgg atcatcttct
     3781 gtggcgagca catgatggat cagcacgagc ggaaccacat cgcagggtat tggaagcaag
     3841 tcggacccat tgccgttggc tccttctgcc tcttcatatt tgacatgtgt gagagagggg
     3901 tacaactcac gaatcccttc tacagtatct ggactacaga cattggaaca gagctggcca
     3961 tggccttcat catcgtggct ggaatctgcc tctgcctcta cttcctgttt ctatgcttca
     4021 tggtatttca ggtgtttcgg aacatcagtg ggaagcagtc cagcctgcca gctatgagca
     4081 aagtccggcg gctacactat gaggggctaa tttttaggtt caagttcctc atgcttatca
     4141 ccttggcctg cgctgccatg actgtcatct tcttcatcgt tagtcaggta acggaaggcc
     4201 attggaaatg gggcggcgtc acagtccaag tgaacagtgc ctttttcaca ggcatctatg
     4261 ggatgtggaa tctgtatgtc tttgctctga tgttcttgta tgcaccatcc cataaaaact
     4321 atggagaaga ccagtccaat ggcgatctgg gtgtccatag tggggaagaa ctccagctca
     4381 ccaccactat cacccatgtg gacggaccca ctgagatcta caagttgacc cgcaaggagg
     4441 cccaggagta cccatacgac gtacctgatt atgcaactta tccttatgat gtacctgatt
     4501 atgctagcta tccttatgat gtcccagact acgcatagga tcccccctct ccctcccccc
     4561 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     4621 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     4681 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     4741 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     4801 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     4861 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     4921 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     4981 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     5041 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     5101 gaaaaacacg atgataagct tgccacaacc catggtgagc aagggcgagg agctgttcac
     5161 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     5221 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     5281 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     5341 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     5401 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     5461 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     5521 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     5581 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     5641 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     5701 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     5761 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     5821 cactctcggc atggacgagc tgtacaagta gtctagataa ttctaccggg taggggaggc
     5881 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     5941 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     6001 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     6061 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     6121 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     6181 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     6241 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     6301 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     6361 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     6421 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6481 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6541 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6601 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6661 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6721 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6781 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6841 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     6901 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     6961 tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa
     7021 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7081 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7141 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7201 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7261 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7321 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7381 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     7441 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7501 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7561 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa
     7621 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     7681 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     7741 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa
     7801 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     7861 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
     7921 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
     7981 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     8041 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
     8101 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
     8161 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
     8221 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     8281 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8341 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8401 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     8461 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt
     8521 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     8581 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8641 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8701 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8761 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8821 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8881 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8941 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     9001 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9061 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     9121 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     9181 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9241 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9301 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9361 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     9421 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9481 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9541 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9601 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9661 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9721 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9781 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9841 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9901 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9961 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    10021 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    10081 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    10141 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    10201 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    10261 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10321 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10381 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10441 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10501 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10561 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10621 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10681 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt
    10741 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    10801 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    10861 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
    10921 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
    10981 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa
    11041 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
