LOCUS       V013955                 9129 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013955
VERSION     V013955
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2846..2887
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             2894..3850
                     /label="TurboID"
                     /note="mutant of the E. coli biotin ligase BioID engineered
                     for improved catalytic efficiency (Branon et al., 2018)"
     CDS             3857..3883
                     /label="NES"
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     promoter        3917..4416
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4437..5033
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    5050..5638
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5845..6478
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6607..6623)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6631..6647)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6655..6685)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6700..6721)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7009..7597)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7771..8628)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8629..8733)
                     /label="AmpR promoter"
     polyA_signal    8781..8915
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgggcaa gcccatcccc aaccccctgc tgggcctgga
     2881 cagcaccgct agcaaagaca atactgtgcc tctgaagctg atcgctctcc tggctaatgg
     2941 cgagttccat agtggcgaac agctgggaga aaccctgggc atgtccaggg ccgctatcaa
     3001 caagcacatt cagactctgc gcgactgggg cgtggacgtg ttcaccgtgc ccggaaaggg
     3061 ctactctctg cccgagccta tcccgctgct gaacgctaaa cagattctgg gacagctgga
     3121 cggcgggagc gtggcagtcc tgcctgtggt cgactccacc aatcagtacc tgctggatcg
     3181 aatcggcgag ctgaagagtg gggatgcttg cattgcagaa tatcagcagg cagggagagg
     3241 aagcagaggg aggaaatggt tctctccttt tggagctaac ctgtacctga gtatgttttg
     3301 gcgcctgaag cggggaccag cagcaatcgg cctgggcccg gtcatcggaa ttgtcatggc
     3361 agaagcgctg cgaaagctgg gagcagacaa ggtgcgagtc aaatggccca atgacctgta
     3421 tctgcaggat agaaagctgg caggcatcct ggtggagctg gccggaataa caggcgatgc
     3481 tgcacagatc gtcattggcg ccgggattaa cgtggctatg aggcgcgtgg aggaaagcgt
     3541 ggtcaatcag ggctggatca cactgcagga agcagggatt aacctggaca ggaatactct
     3601 ggccgctacg ctgatccgag agctgcgggc agccctggaa ctgttcgagc aggaaggcct
     3661 ggctccatat ctgccacggt gggagaagct ggataacttc atcaatagac ccgtgaagct
     3721 gatcattggg gacaaagaga ttttcgggat tagccggggg attgataaac agggagccct
     3781 gctgctggaa caggacggag ttatcaaacc ctggatgggc ggagaaatca gtctgcggtc
     3841 tgccgaaaag ctgcagctgc ctcccctgga gcgcctgacc ctggactagg atcccgcgac
     3901 tctagataat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     3961 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     4021 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     4081 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     4141 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     4201 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     4261 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     4321 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     4381 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga
     4441 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     4501 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     4561 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     4621 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     4681 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     4741 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     4801 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     4861 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     4921 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     4981 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaccgc
     5041 gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5101 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5161 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5221 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5281 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5341 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5401 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     5461 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5521 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5581 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     5641 ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     5701 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     5761 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5821 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata
     5881 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     5941 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     6001 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     6061 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     6121 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     6181 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     6241 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     6301 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6361 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6421 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     6481 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     6541 agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga gcttggcgta
     6601 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6661 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6721 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6781 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6841 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6901 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6961 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7021 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7081 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7141 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7201 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7261 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7321 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7381 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7441 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7501 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7561 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7621 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7681 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7741 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7801 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7861 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7921 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7981 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8041 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8101 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8161 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8221 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8281 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8341 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8401 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8461 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8521 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8581 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8641 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8701 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8761 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
     8821 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8881 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
     8941 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
     9001 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
     9061 tatgtactac aaacttagta gtttttaaag aaattgtatt tgttaaatat gtactacaaa
     9121 cttagtagt
//
