LOCUS       V021480                 9633 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021480
VERSION     V021480
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..4284
                     /label="IFIT3(NM_001549)"
                     /note="IFIT3(NM_001549)"
                     /gene="IFIT3"
     misc_feature    4304..4876
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES"
     CDS             4878..5588
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     misc_feature    5602..6190
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6397..7030
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7158..7174)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7182..7198
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7206..7236)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7251..7272
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7560..8148)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8319..9179)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9180..9284)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9332..9466
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgagt
     2821 gaggtcacca agaattccct ggagaaaatc cttccacagc tgaaatgcca tttcacctgg
     2881 aacttattca aggaagacag tgtctcaagg gatctagaag atagagtgtg taaccagatt
     2941 gaatttttaa acactgagtt caaagctaca atgtacaact tgttggccta cataaaacac
     3001 ctagatggta acaacgaggc agccctggaa tgcttacggc aagctgaaga gttaatccag
     3061 caagaacatg ctgaccaagc agaaatcaga agtctagtca cttggggaaa ctacgcctgg
     3121 gtctactatc acttgggcag actctcagat gctcagattt atgtagataa ggtgaaacaa
     3181 acctgcaaga aattttcaaa tccatacagt attgagtatt ctgaacttga ctgtgaggaa
     3241 gggtggacac aactgaagtg tggaagaaat gaaagggcga aggtgtgttt tgagaaggct
     3301 ctggaagaaa agcccaacaa cccagaattc tcctctggac tggcaattgc gatgtaccat
     3361 ctggataatc acccagagaa acagttctct actgatgttt tgaagcaggc cattgagctg
     3421 agtcctgata accaatacgt caaggttctc ttgggcctga aactgcagaa gatgaataaa
     3481 gaagctgaag gagagcagtt tgttgaagaa gccttggaaa agtctccttg ccaaacagat
     3541 gtcctccgca gtgcagccaa attttacaga agaaaaggtg acctagacaa agctattgaa
     3601 ctgtttcaac gggtgttgga atccacacca aacaatggct acctctatca ccagattggg
     3661 tgctgctaca aggcaaaagt aagacaaatg cagaatacag gagaatctga agctagtgga
     3721 aataaagaga tgattgaagc actaaagcaa tatgctatgg actattcgaa taaagctctt
     3781 gagaagggac tgaatcctct gaatgcatac tccgatctcg ctgagttcct ggagacggaa
     3841 tgttatcaga caccattcaa taaggaagtc cctgatgctg aaaagcaaca atcccatcag
     3901 cgctactgca accttcagaa atataatggg aagtctgaag acactgctgt gcaacatggt
     3961 ttagagggtt tgtccataag caaaaaatca actgacaagg aagagatcaa agaccaacca
     4021 cagaatgtat ctgaaaatct gcttccacaa aatgcaccaa attattggta tcttcaagga
     4081 ttaattcata agcagaatgg agatctgctg caagcagcca aatgttatga gaaggaactg
     4141 ggccgcctgc taagggatgc cccttcaggc ataggcagta ttttcctgtc agcatctgag
     4201 cttgaggatg gtagtgagga aatgggccag ggcgcagtca gctccagtcc cagagagctc
     4261 ctctctaact cagagcaact gaactgagcg gccgcggatc ccgcccctct ccctcccccc
     4321 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     4381 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     4441 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     4501 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     4561 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     4621 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     4681 ttgtggaaag agtcaaatgg ctcacctcaa gcgtattcaa caaggggctg aaggatgccc
     4741 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     4801 tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     4861 aaaaacacga tgataatatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     4921 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     4981 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     5041 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     5101 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     5161 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     5221 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     5281 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     5341 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     5401 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     5461 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     5521 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     5581 acaagtgaac gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
     5641 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5701 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5761 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5821 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5881 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5941 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     6001 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6061 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6121 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6181 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa
     6241 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     6301 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     6361 tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac
     6421 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     6481 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     6541 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     6601 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     6661 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     6721 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     6781 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     6841 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6901 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6961 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7021 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7081 tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac ctctagctag
     7141 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7201 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7261 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7321 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7381 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7441 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7501 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7561 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7621 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7681 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7741 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7801 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7861 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7921 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7981 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8041 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8101 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8161 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8221 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8281 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8341 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8401 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8461 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8521 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8581 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8641 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8701 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8761 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8821 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8881 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     8941 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9001 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9061 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9121 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9181 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9241 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9301 gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta
     9361 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9421 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     9481 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
     9541 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
     9601 tatttgttaa atatgtacta caaacttagt agt
//
