LOCUS       V013809                10174 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013809
VERSION     V013809
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2338..3519
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3548..4804
                     /gene="N"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Severe acute respiratory syndrome
                     coronavirus 2. Accession#: P0DTC9"
     CDS             4829..4852
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
     CDS             4889..4912
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
     misc_feature    4924..5510
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5530..6126
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6143..6731
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6938..7571
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7747..7777)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7792..7813)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8101..8689)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8863..9720)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9721..9825)
                     /label="AmpR promoter"
     polyA_signal    9873..10004
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtacgaatt
     3541 cgccaccatg agcgataacg gcccccaaaa ccaacggaat gcccctcgga taacatttgg
     3601 tggccctagt gattccacag gaagtaacca aaacggagaa cggtctggag cccgctctaa
     3661 acagaggcga ccacagggat tgccaaataa cacagcgtca tggtttacag ccctgactca
     3721 acatggaaag gaggacttga aatttccacg aggacagggt gtaccaataa acacaaacag
     3781 tagtccagac gatcagatcg ggtattatag aagggccacg agacgcatca gaggtggaga
     3841 tggtaaaatg aaagatctca gtccgcgctg gtacttttat tatttgggaa ccggcccaga
     3901 agcgggcctc ccatatggag ccaataagga cgggattata tgggtggcta cagagggagc
     3961 cctcaatacg ccaaaagatc acatcggaac aagaaaccct gctaataatg ccgcaatcgt
     4021 gctgcagttg cctcagggga cgacgcttcc taaaggcttt tacgcagaag gatcacgcgg
     4081 cggcagccaa gcatcctcaa ggtctagttc cagaagtcga aacagctcca gaaactccac
     4141 accagggtcc agtaggggca caagtccggc gcggatggcg ggcaacggcg gagacgccgc
     4201 actcgctctg ttgctcctgg accggctcaa ccaacttgaa tccaagatga gtggtaaggg
     4261 acagcagcaa caaggtcaaa ccgtaaccaa gaaaagcgct gcagaagctt ctaaaaaacc
     4321 tcgacaaaaa cggaccgcta cgaaggcata taacgttact caagccttcg ggagacgagg
     4381 gccggagcaa acccagggga attttggaga tcaggagctc atccggcaag ggacagatta
     4441 caaacattgg cctcaaatcg ctcagttcgc gccgtccgca agtgcttttt tcggaatgtc
     4501 tcgcatcggt atggaagtaa caccatccgg gacatggctc acttataccg gcgccatcaa
     4561 gctggacgac aaagatccaa atttcaaaga tcaggtgatt ctcttgaata aacacataga
     4621 cgcgtataag acgtttccac ccaccgaacc caagaaggac aaaaagaaaa aagctgatga
     4681 aacacaagcg ttgccccagc gacagaagaa gcagcaaacc gtcaccctcc tgccagccgc
     4741 tgatctcgat gacttctcca agcaacttca acagtccatg agctcagccg attctactca
     4801 ggcgggccgc ctcgaaggcg gcgggggatg gagccatcca caattcgaga aaggcggtgg
     4861 ttcaggagga ggtagcgggg gtggatcatg gtcacatccg cagtttgaaa agtaaggatc
     4921 ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     4981 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     5041 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     5101 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     5161 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     5221 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     5281 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     5341 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     5401 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     5461 cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc cacaaggaga
     5521 cgaccttcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     5581 cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     5641 gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     5701 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     5761 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     5821 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     5881 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     5941 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     6001 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     6061 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     6121 ggtgcctaga cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac
     6181 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6241 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6301 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6361 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6421 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6481 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     6541 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6601 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6661 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     6721 cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca
     6781 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     6841 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     6901 gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa
     6961 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
     7021 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
     7081 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
     7141 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
     7201 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
     7261 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
     7321 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
     7381 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7441 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7501 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7561 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7621 atgaatatca gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga cctctagcta
     7681 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7741 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7801 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7861 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7921 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7981 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8101 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8341 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8401 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8461 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8521 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8581 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8701 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8761 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9841 agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt
     9901 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9961 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
    10021 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
    10081 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
    10141 gtatttgtta aatatgtact acaaacttag tagt
//
