LOCUS       V016721                10447 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V016721
VERSION     V016721
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2283..3460
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3488..4204
                     /label="Cycle 3 GFP"
                     /note="Cycle 3 GFP (Crameri et al., 1996)"
     protein_bind    4226..4250
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4259..4285
                     /label="NES"
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     misc_feature    5023..5596
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5630..6337
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     LTR             7165..7798
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7975..8005)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8020..8041)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8329..8914)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9088..9945)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9946..10050)
                     /label="AmpR promoter"
     polyA_signal    10098..10232
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgcgccc gacgataagc tttgcaaaga tggataaagt
     2221 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
     2281 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2341 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2401 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2461 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2521 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2581 ttacttccac tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     2641 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     2701 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     2761 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     2821 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
     2881 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     2941 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     3001 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     3061 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     3121 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     3181 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     3241 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     3301 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     3361 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     3421 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaatttcga cccggatccg
     3481 cggccgcatg gccagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
     3541 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     3601 tacatacgga aagcttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     3661 gccaacactt gtcactactt tctcttatgg tgttcaatgc ttttcccgtt atccggatca
     3721 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac
     3781 tatatctttc aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga
     3841 tacccttgtt aatcgtatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     3901 cggacacaaa ctcgagtaca actataactc acacaatgta tacatcacgg cagacaaaca
     3961 aaagaatgga atcaaagcta acttcaaaat tcgccacaac attgaagatg gatccgttca
     4021 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     4081 caaccattac ctgtcgacac aatctgccct ttcgaaagat cccaacgaaa agcgtgacca
     4141 catggtcctt cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta
     4201 caaaagcggt tccggaccgg atcaaacaag tttgtacaaa aaagcaggct tactacagct
     4261 accgccgctg gaaagactga ctctgggagg cgggatggtg agcaagggcg aggagctgtt
     4321 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     4381 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     4441 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     4501 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     4561 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     4621 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     4681 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     4741 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     4801 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     4861 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     4921 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     4981 gatcactctc ggcatggacg agctgtacaa gtaggaattc cgcccctctc cctccccccc
     5041 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     5101 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     5161 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     5221 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     5281 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     5341 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     5401 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     5461 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     5521 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     5581 aaaaacacga tgataagctt gccgtacgat gccaaaaaag aaaagaaaag ttgtgagcaa
     5641 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     5701 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     5761 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     5821 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     5881 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     5941 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     6001 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     6061 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     6121 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     6181 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     6241 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     6301 ccgccactcc accggcggca tggacgagct gtacaagtcc ggactcagat ctcgagctca
     6361 catcacaagt ttgtacaaaa aagcaggctt aatgagcgag ctgattaagg agaacatgca
     6421 catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg
     6481 cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc
     6541 tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca gaaccttcat
     6601 caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccctgagg gcttcacatg
     6661 ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct
     6721 ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc catccaacgg
     6781 ccctgtgatg cagaagaaaa cactcggctg ggaggccaac accgagatgc tgtaccccgc
     6841 tgacggcggc ctggaaggca gaagcgacat ggccctgaag ctcgtgggcg ggggccacct
     6901 gatctgcaac ttcaagacca catacagatc caagaaaccc gctaagaacc tcaagatgcc
     6961 cggcgtctac tatgtggacc acagactgga aagaatcaag gaggccgaca aagagaccta
     7021 cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca aactggggca
     7081 caaacttaat tagggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7141 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata
     7201 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     7261 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     7321 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     7381 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     7441 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     7501 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     7561 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     7621 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7681 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7741 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7801 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     7861 agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga gcttggcgta
     7921 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7981 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8041 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8101 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8161 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8221 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8281 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8341 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8401 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8461 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8521 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8581 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8641 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8701 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8761 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8821 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa
     8881 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8941 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9001 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9061 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9121 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9181 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9241 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9301 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9361 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9421 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9481 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9541 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9601 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9661 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9721 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9781 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9841 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9901 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9961 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10021 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10081 cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    10141 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    10201 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    10261 atcccaaact tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta
    10321 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
    10381 tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt actacaaact
    10441 tagtagt
//
