LOCUS       V013897                 9553 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013897
VERSION     V013897
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2338..3519
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3577..3600
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    3610..4196
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4198..4890
                     /label="TurboRFP"
                     /note="red fluorescent protein from Entacmaea quadricolor"
     CDS             4909..5505
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    5522..6110
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6317..6950
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7078..7094)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7102..7118)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7126..7156)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7171..7192)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7480..8068)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8242..9099)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9100..9204)
                     /label="AmpR promoter"
     polyA_signal    9252..9386
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     3541 ctcgagacta gttctagagc ggccgctagc ggatccgatt acaaggatga cgacgataag
     3601 tagatcccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     3661 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     3721 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     3781 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     3841 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     3901 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     3961 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     4021 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     4081 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     4141 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccatg
     4201 agcgagctga tcaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac
     4261 caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg
     4321 aagatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc
     4381 ttcatgtacg gcagcaaagc cttcatcaac cacacccagg gcatccccga cttctttaag
     4441 cagtccttcc ctgagggctt cacatgggag agaatcacca catacgaaga cgggggcgtg
     4501 ctgaccgcta cccaggacac cagcttccag aacggctgca tcatctacaa cgtcaagatc
     4561 aacggggtga acttcccatc caacggccct gtgatgcaga agaaaacacg cggctgggag
     4621 gccaacaccg agatgctgta ccccgctgac ggcggcctga gaggccacag ccagatggcc
     4681 ctgaagctcg tgggcggggg ctacctgcac tgctccttca agaccacata cagatccaag
     4741 aaacccgcta agaacctcaa gatgcccggc ttccacttcg tggaccacag actggaaaga
     4801 atcaaggagg ccgacaaaga gacctacgtc gagcagcacg agatggctgt ggccaagtac
     4861 tgcgacctcc ctagcaaact ggggcacaga acaaggagac gaccttccat gaccgagtac
     4921 aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc
     4981 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag
     5041 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     5101 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     5161 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     5221 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     5281 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     5341 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     5401 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     5461 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctagac gcgtctggaa
     5521 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5581 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     5641 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     5701 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     5761 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     5821 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     5881 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     5941 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6001 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6061 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa
     6121 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     6181 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     6241 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6301 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     6361 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     6421 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     6481 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     6541 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     6601 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     6661 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     6721 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     6781 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6841 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6901 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     6961 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7021 gccttgacat tgctagcgtt taccgtcgac ctctagctag agcttggcgt aatcatggtc
     7081 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7141 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7201 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7261 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7321 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7381 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7441 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7501 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7561 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7621 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7681 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7741 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7801 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7861 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7921 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7981 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8041 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8101 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8161 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8221 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8281 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8341 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8401 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8461 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8521 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8581 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8641 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8701 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8761 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8821 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8881 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8941 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9001 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9061 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9121 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9181 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     9241 atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9301 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9361 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     9421 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     9481 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     9541 caaacttagt agt
//
